FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_34.34100000009ad1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_34.34100000009ad1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051311
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC37990.3613583421080917No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA13610.1294574107947125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4850.042.2150745
TATGCCG5650.035.83949343
ATGCCGT5750.035.216244
CGAGACT5850.034.2296123
ACGAGAC6150.034.0232422
CCACGAG6150.034.0232420
GTATGCC6200.033.3859742
TCTCGTA6200.033.3859738
CACGAGA6300.033.21316521
CGTATGC6250.033.11887741
TCGTATG6450.032.09193840
CCCACGA6550.031.94548419
ATCTCGT6500.031.84507437
CTCGTAT6500.031.49893239
GACTTGC6350.030.47140926
CGAGACG453.491369E-529.99898723
CGCGCTT300.005147912529.99898512
GCCCACG7100.029.47083518
CTTGCTG6650.029.0967628
AATCTCG6750.028.9990236
Produced by FastQC (version 0.11.2)