FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_33.34100000009ac4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_33.34100000009ac4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451433
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC61450.42337469245910764No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT18340.1263578821757532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6850.040.73011445
CGACGCT8500.032.2931528
CCGTCGG352.8212345E-432.141913
GCCCACG8800.031.95927218
ACGACGC8650.031.73315427
ATGCCGT8850.031.52556844
CGAGACG9050.031.07642223
CGCTATA9000.030.74908431
TATGCCG9100.030.65948143
CGTATGC9250.030.404541
CCCACGA9300.030.24103419
CGGCCGT300.00514845129.99910410
GCCAGGC300.00514845129.99910427
AGCCCAC9400.029.91932117
CGAGCCC9450.029.76101515
GACGCTA9350.029.59804529
GTATGCC9650.028.91105542
CCGAGCC9750.028.84529314
ACGCTAT9800.028.69812630
ACGAGAC10050.028.4319922
Produced by FastQC (version 0.11.2)