FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_32.34100000009ab7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_32.34100000009ab7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398997
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC46190.33016511114748637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5900.035.46477545
GCCCACG6300.033.92730718
TATGCCG6350.033.30584343
ATGCCGT6500.032.1911144
AGCCCAC6850.031.20321517
GGCGAAT6350.031.17993732
CCGAGCC6800.031.1017814
CGAGCCC6950.030.75424815
CAAGGCG6450.030.6965329
TCGCCCC300.00514857629.99887825
CCCACGA7200.029.68639219
GAGCCCA7300.029.2797316
GTATGCC7250.029.17132642
CGTATGC7200.029.06141541
CCACGAG7400.028.88405620
AGGCGAA6900.028.36850731
GCGCGCT406.1681645E-428.12395111
GCGAATC7050.028.08405933
TCCGAGC7700.027.46650713
CACGAGA8250.026.45355821
Produced by FastQC (version 0.11.2)