FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_31.34100000009aab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_31.34100000009aab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496014
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC85600.5721871586763225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9400.041.64927345
CGAGCCC11550.034.28484715
GCCCACG11500.034.23826618
ATGCCGT11550.034.0911944
TATGCCG11650.033.7985643
CCGAGCC11650.033.79743214
CCCACGA11800.033.367819
GTATGCC12050.032.8622442
TAACCTA11950.032.572431
CGAGACG11950.032.572423
AGCCCAC12200.032.458217
CCACGAG12150.032.4065920
CGTATGC12200.032.27377741
TCCGAGC12700.031.18031513
GAGCCCA12900.030.696916
ACGAGAC13150.030.11330822
CCTCGCG453.4921435E-529.99924338
CTCGCGG453.4921435E-529.99924339
CTCGTAT13200.029.82879339
TATCTCG13200.029.82879336
Produced by FastQC (version 0.11.2)