FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_30.34100000009a9e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_30.34100000009a9e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC271.7142857142857144No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC221.3968253968253967No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC161.0158730158730158No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC130.8253968253968255No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC90.5714285714285714No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT70.4444444444444444No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC40.25396825396825395No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT40.25396825396825395No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT30.19047619047619047No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT30.19047619047619047No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC30.19047619047619047No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT30.19047619047619047No Hit
CTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGAGAGGTGTAG20.12698412698412698No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGAC20.12698412698412698No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC20.12698412698412698RNA PCR Primer, Index 46 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG20.12698412698412698No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)