##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_1_3.34100000009826.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 133037 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.935829881912554 38.0 38.0 38.0 32.0 38.0 2 36.94399302449695 38.0 38.0 38.0 32.0 38.0 3 36.92162330780159 38.0 38.0 38.0 32.0 38.0 4 36.966054556251265 38.0 38.0 38.0 32.0 38.0 5 36.9548321143742 38.0 38.0 38.0 32.0 38.0 6 38.910506099806824 40.0 38.0 40.0 38.0 40.0 7 38.84947044807084 40.0 38.0 40.0 38.0 40.0 8 38.78230116433774 40.0 38.0 40.0 38.0 40.0 9 38.79868758315356 40.0 38.0 40.0 38.0 40.0 10 38.759660846230744 40.0 38.0 40.0 38.0 40.0 11 38.75620316152649 40.0 38.0 40.0 38.0 40.0 12 38.76190834128851 40.0 38.0 40.0 38.0 40.0 13 38.75266279305757 40.0 38.0 40.0 38.0 40.0 14 38.72210738366019 40.0 38.0 40.0 38.0 40.0 15 38.78204559633786 40.0 38.0 40.0 38.0 40.0 16 38.78264693280816 40.0 38.0 40.0 38.0 40.0 17 38.771251606695884 40.0 38.0 40.0 38.0 40.0 18 38.7900508880988 40.0 38.0 40.0 38.0 40.0 19 38.80951915632493 40.0 38.0 40.0 38.0 40.0 20 38.76819982410908 40.0 38.0 40.0 38.0 40.0 21 38.789908070687105 40.0 38.0 40.0 38.0 40.0 22 38.790599607627954 40.0 38.0 40.0 38.0 40.0 23 38.78393980621932 40.0 38.0 40.0 38.0 40.0 24 38.77375466975353 40.0 38.0 40.0 38.0 40.0 25 38.76762103775641 40.0 38.0 40.0 38.0 40.0 26 38.72019062366109 40.0 38.0 40.0 38.0 40.0 27 38.66730308109774 40.0 38.0 40.0 38.0 40.0 28 38.632245164878945 40.0 38.0 40.0 38.0 40.0 29 38.551681111269794 40.0 38.0 40.0 38.0 40.0 30 38.47259784871878 40.0 38.0 40.0 38.0 40.0 31 38.35699842900847 40.0 38.0 40.0 38.0 40.0 32 38.22668129918745 40.0 38.0 40.0 38.0 40.0 33 38.04899388891812 40.0 38.0 40.0 38.0 40.0 34 37.89752474875411 40.0 38.0 40.0 38.0 40.0 35 37.71785292813278 40.0 38.0 40.0 32.0 40.0 36 37.504664115997805 40.0 38.0 40.0 32.0 40.0 37 37.39705495463668 40.0 38.0 40.0 32.0 40.0 38 37.255109480821126 40.0 38.0 40.0 32.0 40.0 39 37.08946383336966 40.0 38.0 40.0 32.0 40.0 40 36.91993204897886 40.0 38.0 40.0 27.0 40.0 41 36.73012770883288 40.0 38.0 40.0 27.0 40.0 42 36.51360899599359 40.0 38.0 40.0 27.0 40.0 43 36.246172117531216 40.0 38.0 40.0 27.0 40.0 44 35.96491201695769 40.0 38.0 40.0 13.0 40.0 45 35.633756022760586 40.0 38.0 40.0 13.0 40.0 46 35.29690988221322 40.0 38.0 40.0 13.0 40.0 47 34.946886956260286 40.0 38.0 40.0 13.0 40.0 48 34.55858144726655 40.0 38.0 40.0 13.0 40.0 49 34.210339980606896 40.0 32.0 40.0 13.0 40.0 50 33.93858851297008 40.0 32.0 40.0 13.0 40.0 51 33.34193495042732 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.31127834895251993 2201 2 -0.1403715726110093 2201 3 -0.14695563692595215 2201 4 -0.39434161020560055 2201 5 0.035623422114483105 2201 6 0.12153510971926806 2201 7 0.36897192546970814 2201 8 0.26837071691975467 2201 9 0.34978244126162394 2201 10 0.2214788883910117 2201 11 -0.0025329137781469058 2201 12 0.06949869630310701 2201 13 -0.11756952749068716 2201 14 0.0927236583289428 2201 15 0.0950113493940492 2201 16 -0.03945751321196411 2201 17 0.10402387705567406 2201 18 0.06336722315333532 2201 19 0.4721750805761218 2201 20 0.23193910894901393 2201 21 0.41791348153559227 2201 22 0.35504952753726826 2201 23 0.22271747825183041 2201 24 -0.18251155486102988 2201 25 -0.1389664213335351 2201 26 -0.23875164939386195 2201 27 -0.2900664719034367 2201 28 -0.09244415541159867 2201 29 -0.020362167806297293 2201 30 -0.10827099163773113 2201 31 0.10266007186916681 2201 32 0.06461783255326026 2201 33 0.28549306385350093 2201 34 0.21935895592299204 2201 35 -0.013878473270509062 2201 36 0.3533941819257933 2201 37 -0.07476988474887492 2201 38 0.1057469213108817 2201 39 0.12657931857135196 2201 40 0.32827345514437667 2201 41 -0.48337085912138633 2201 42 0.25884858411853884 2201 43 -0.2035518471334754 2201 44 -1.0648008096327501 2201 45 -0.8691583838222812 2201 46 -0.2909692720083612 2201 47 -0.4837290611506404 2201 48 -1.0288166991986785 2201 49 -0.8159465416394696 2201 50 -0.44131728818953064 2201 51 -0.5195073137481003 1101 1 -0.23957947392936774 1101 2 -0.06605997960581789 1101 3 0.1544558866600454 1101 4 -0.013329412261732898 1101 5 -0.06559637227248061 1101 6 -0.3280368052587477 1101 7 -0.2048934012963457 1101 8 0.25848674614192646 1101 9 0.07762614890765462 1101 10 0.2934434515459472 1101 11 0.06622514380672584 1101 12 -0.006366564349434611 1101 13 0.07019286052954499 1101 14 -0.10931924934352821 1101 15 0.061524853079873765 1101 16 7.064483099696872E-4 1101 17 0.14217137631190013 1101 18 -0.07428111905022661 1101 19 0.3987890768076525 1101 20 0.19118012577490617 1101 21 0.0017950061132907535 1101 22 -0.05677156928597782 1101 23 0.0965067744734398 1101 24 0.2793244175035241 1101 25 0.056332907614269345 1101 26 0.401888329449811 1101 27 -0.20739151900931319 1101 28 -0.360137785374242 1101 29 0.14869009492881702 1101 30 0.03519534005378944 1101 31 -0.1456292408808295 1101 32 0.26004938853488113 1101 33 0.3589782378973325 1101 34 0.19826874402917127 1101 35 0.38481909044639906 1101 36 -0.37478009330477846 1101 37 0.06281234393777879 1101 38 0.5154193288345965 1101 39 0.4367178263892697 1101 40 -0.015549889538441164 1101 41 0.3890631100035975 1101 42 0.069962266310867 1101 43 0.6028677753583409 1101 44 0.3923080462728379 1101 45 0.8351158550458564 1101 46 1.077382187447526 1101 47 0.8009887994159541 1101 48 0.007149053999562227 1101 49 0.3449407017574444 1101 50 -0.11121249826523183 1101 51 0.08324961990698654 2202 1 0.3453677638111685 2202 2 0.06758371995966428 2202 3 0.15386619081895248 2202 4 0.05911006632299376 2202 5 -0.19843311963436605 2202 6 0.22975338716831573 2202 7 -0.45321742364271245 2202 8 -0.07521902666999125 2202 9 -0.4162596363189124 2202 10 -0.5514665422467289 2202 11 -0.3328747941200234 2202 12 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-1.0787146579244151 1213 38 -0.7170556813325746 1213 39 -0.8652364106449113 1213 40 -1.0640954491608667 1213 41 -1.12274150910212 1213 42 -0.7521015621101199 1213 43 -0.7858819838402695 1213 44 -0.9522840896889733 1213 45 -0.5672472092320646 1213 46 -0.7691256573265477 1213 47 -0.7194632650545998 1213 48 -1.1022710962605018 1213 49 -0.21307637908760313 1213 50 -0.9669243071652502 1213 51 -1.1922271668393023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 6.0 16 16.0 17 12.0 18 8.0 19 19.0 20 13.0 21 29.0 22 47.0 23 111.0 24 194.0 25 315.0 26 513.0 27 810.0 28 1247.0 29 1606.0 30 2342.0 31 3156.0 32 3558.0 33 4273.0 34 5212.0 35 5981.0 36 7329.0 37 10082.0 38 15618.0 39 70539.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.14588979223126 20.54351099066546 13.80006022282445 17.510538994278832 2 14.392988416756241 24.863008035358586 43.05493960326827 17.689063944616912 3 20.22219382577779 33.70490916060945 28.14254681028586 17.930350203326896 4 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18.054375850327354 27.41718469298015 30.7711388561077 23.7573006005848 18 21.562422483970625 25.394439140990855 33.8740350428828 19.169103332155714 19 22.64106977758067 25.00357043529244 34.837676736546975 17.51768305057991 20 21.21515067236934 24.790095988334073 34.70387937190406 19.29087396739253 21 20.713034719664456 26.2731420582244 35.218022054015044 17.79580116809609 22 18.847388320542404 27.255575516585612 35.80357344197479 18.093462720897193 23 18.710584273547962 28.489066951299264 35.70435292437442 17.095995850778355 24 18.418184414862033 29.18661725685336 35.55702548914964 16.838172839134977 25 17.143351097814893 29.81351052714658 36.33124619466765 16.711892180370874 26 16.715650533310285 30.907191232514265 35.66752106556822 16.70963716860723 27 16.150394251223343 30.827514150198816 36.082443230078844 16.93964836849899 28 15.92263806309523 31.118410667708986 34.98274916000812 17.97620210918767 29 16.09477062772011 31.32512007937641 34.66930252486151 17.910806768041972 30 16.620188368649323 31.010170103054037 34.00858407811361 18.361057450183033 31 17.11328427429963 30.658388267925464 33.683862384148775 18.544465073626135 32 17.018573780226554 30.53135593857348 34.0048257251742 18.445244556025767 33 16.926869968505002 30.75610544435007 35.059419559972035 17.257605027172893 34 16.462337545194195 31.341656832309813 34.15215316039898 18.04385246209701 35 16.78555589798327 31.83174605560859 33.57637349008171 17.806324556326437 36 16.702120462728416 32.34288205536806 33.91763193697994 17.037365544923595 37 16.393935521696974 32.49396784353225 33.78157956057338 17.330517074197406 38 16.03764366304111 31.745303938002213 34.33405744266633 17.882994956290354 39 15.914369686628532 31.744552267414328 33.88080007817374 18.460277967783398 40 15.697136886730759 31.685170290971687 34.11682464276855 18.500868179529004 41 16.051173733622978 31.708472079196014 33.55683005479679 18.683524132384225 42 15.888061216052677 31.52882280869232 33.15769297263167 19.42542300262333 43 15.391958628050842 31.211617820606296 32.89235325510948 20.50407029623338 44 14.822943993024499 30.74858873847125 32.74577749047258 21.682689778031676 45 14.740260228357524 30.250231138705775 32.812676172794035 22.19683246014267 46 15.420522110390342 29.52186233904854 32.60671843171449 22.450897118846637 47 14.897359381224772 29.318911280320513 32.12715259664605 23.656576741808667 48 14.492960604944487 28.98141118636169 32.05123386727001 24.474394341423814 49 15.00165373744663 28.875759215827774 31.57961994106681 24.54296710565879 50 14.458383757901938 28.5446905748025 31.551372926328764 25.445552740966797 51 14.514007381405172 28.207190480843675 30.715515232604464 26.56328690514669 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 95.0 1 56.5 2 18.0 3 36.0 4 54.0 5 74.0 6 94.0 7 111.5 8 129.0 9 193.5 10 258.0 11 533.5 12 809.0 13 1858.5 14 2908.0 15 3697.5 16 4487.0 17 4599.5 18 4712.0 19 4435.5 20 4159.0 21 4147.0 22 4135.0 23 4491.0 24 4847.0 25 4645.0 26 3657.5 27 2872.0 28 3033.5 29 3195.0 30 4669.0 31 6143.0 32 8371.0 33 10599.0 34 10618.0 35 10637.0 36 9432.5 37 8228.0 38 6806.5 39 5385.0 40 4547.0 41 3709.0 42 3590.5 43 3472.0 44 3502.5 45 3533.0 46 3729.0 47 3925.0 48 4440.0 49 4955.0 50 5057.0 51 5159.0 52 5037.0 53 4915.0 54 4944.0 55 4973.0 56 4767.5 57 4562.0 58 4233.0 59 3904.0 60 3574.0 61 3244.0 62 2848.5 63 2453.0 64 2132.5 65 1812.0 66 1560.0 67 1308.0 68 1101.5 69 895.0 70 791.5 71 688.0 72 580.5 73 473.0 74 413.5 75 294.5 76 235.0 77 186.0 78 137.0 79 104.5 80 72.0 81 53.5 82 35.0 83 24.0 84 13.0 85 7.0 86 1.0 87 0.5 88 0.0 89 1.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14807910581266867 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.003758352939407834 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 133037.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.9371857411912 #Duplication Level Percentage of deduplicated Percentage of total 1 87.24633593941594 59.27270529954466 2 7.539473324387797 10.244211992593799 3 2.281806319720217 4.650584993047689 4 1.031921374740143 2.804233364241058 5 0.5855997213252665 1.9891998518832217 6 0.3254438881241967 1.32658451254974 7 0.1567008978047594 0.7452072599981351 8 0.15426075767897945 0.838403339761097 9 0.11203912642473156 0.6850460647978011 >10 0.47210771500994847 5.7583294269285945 >50 0.05413244169002836 2.40758471404167 >100 0.03125065277700318 4.252030200793823 >500 0.006695880675723504 3.0645048780249273 >1k 0.0022319602252411684 1.9613741017937867 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1522 1.1440426347557446 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1065 0.8005291760938686 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 828 0.6223832467659374 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 816 0.6133631997113584 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 720 0.5412028232747281 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 583 0.4382239527349534 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 582 0.4374722821470719 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 513 0.38560701158324373 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 450 0.3382517645467051 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 420 0.31570164691025804 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 336 0.25256131752820643 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 335 0.25180964694032487 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 331 0.2488029645887986 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 319 0.2397829175342198 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 260 0.19543435284920738 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 237 0.17814592932793133 No Hit GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 227 0.17062922344911566 No Hit GTATAGATTCTATCCTCGGAAGGAAACAAGAATAGAAGAAGAACCTCTAAT 222 0.16687087050970784 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 217 0.1631125175703 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 196 0.14732743522478708 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 185 0.13905905875808985 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 159 0.11951562347316913 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 159 0.11951562347316913 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 154 0.1157572705337613 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 146 0.10974390583070874 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 7.516705878815667E-4 10 0.0 0.0 0.0015033411757631335 11 0.0 0.0 0.0015033411757631335 12 0.0 0.0 0.0022550117636447003 13 0.0 0.0 0.003006682351526267 14 0.0 0.0 0.003006682351526267 15 0.0 0.0 0.003006682351526267 16 0.0 0.0 0.003758352939407834 17 0.0 0.0 0.003758352939407834 18 0.0 0.0 0.003758352939407834 19 0.0 0.0 0.003758352939407834 20 0.0 0.0 0.008268376466697234 21 0.0 0.0 0.016536752933394468 22 0.0 0.0 0.03758352939407834 23 0.0 0.0 0.09245548230943272 24 0.0 0.0 0.1540924705157212 25 0.0 0.0 0.19167599990979953 26 0.0 0.0 0.2675947292858378 27 0.0 0.0 0.34050677631034976 28 0.0 0.0 0.5231627291655705 29 0.0 0.0 0.8433743996031179 30 0.0 0.0 1.2613032464652691 31 0.0 0.0 1.774694257988379 32 0.0 0.0 2.3241654577298045 33 0.0 0.0 2.908213504513782 34 0.0 0.0 3.4937648924735223 35 0.0 0.0 4.160496703924472 36 0.0 0.0 4.912167291806039 37 0.0 0.0 5.894600750167247 38 0.0 0.0 7.180709126032608 39 0.0 0.0 8.760720701759661 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATACT 20 7.012804E-4 44.99737 4 GACTAGA 45 1.9073923E-8 39.99766 26 AGACTAG 50 4.8261427E-8 35.9979 25 TCCTATC 45 8.627194E-7 34.997955 33 CTAGATC 55 1.115568E-7 32.725357 28 TATCTCG 55 1.115568E-7 32.725357 36 CTAATAC 55 1.115568E-7 32.725357 3 ATCCTAT 50 1.9651525E-6 31.49816 32 CGTATGC 50 1.9651525E-6 31.49816 41 CTCGTAT 50 1.9651525E-6 31.49816 39 CTATCTC 50 1.9651525E-6 31.49816 35 ATGCCGT 50 1.9651525E-6 31.49816 44 TGCCGTC 50 1.9651525E-6 31.49816 45 GCCCACG 60 2.3930806E-7 29.998245 18 TCCGAGG 30 0.005132566 29.998245 33 TCCTGCG 30 0.005132566 29.998245 2 ACTAGAT 60 2.3930806E-7 29.998245 27 TAGATCC 60 2.3930806E-7 29.998245 29 CGAGACT 60 2.3930806E-7 29.998245 23 TCTCGTA 55 4.1305357E-6 28.634687 38 >>END_MODULE