FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_29.34100000009a81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_29.34100000009a81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469800
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC36100.245611647843244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4750.036.94745345
GCCCACG5300.033.5366718
ATGCCGT5250.033.42864644
GACTGCG5250.032.14183826
TATGCCG5750.030.91311343
GCGACCT5400.030.83235530
TGCGACC5550.030.8098329
CTGCGAC5500.030.68084328
CGACCTA5550.029.99904631
CCCACGA5950.029.87300119
CGTATGC6300.028.2133941
GTATGCC6300.028.2133942
ACTGCGA6150.027.80399527
CCGAGCC6400.027.77255614
CGAGACT6450.027.55726423
CCACGAG6650.027.40514620
GACCTAT6050.027.147932
AGCCCAC6650.027.0668117
CGAGCCC6700.026.52900915
ACCTATC6450.026.1619633
Produced by FastQC (version 0.11.2)