FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_28.34100000009a74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_28.34100000009a74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515579
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC457403.0179885047232773No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT102150.6739998376857953No Hit
GTTCCAAAGTGTTCACTTCTTTTTCGCCAGGTTTCATTCGATTTATTGTGG31120.20533406704632357No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG25930.1710897287439322No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG24070.15881719131764163No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG21970.14496110067505555No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGG20240.13354632124092508No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA18740.12364911363907787No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA18550.12239546734284389No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA17710.11685303108580945No Hit
CTCTAAGCCTAAGTATTCCTCAATGACCGATAGCGTACAAGTACCTGTCTC17430.11500555233346463No Hit
GTACTTGTACGCTATCGGTCATTGAGGAATACTTAGGCTTAGAGCTGTCTC16390.10814348839618391No Hit
GTACACGAAAGTGACGAAGTTGCTTTGACTACAAAACCTGTCTCTTATACA15270.10075357338680466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC48450.043.748545
ATGCCGT58850.036.09371644
CTTGCAG62300.034.34436829
CCACGAG62850.034.29440720
CCTTGCA62650.034.22432728
TATGCCG62250.034.15847843
CGTATGC62450.034.08174541
GTATGCC62450.034.08174542
ACCTTGC63050.034.04288527
CCCACGA63350.033.9882219
GCCCACG63450.033.93465418
TCGTATG62950.033.8467840
GACCTTG63500.033.8370726
ACGAGAC64050.033.6518922
CTCGTAT63600.033.39473339
CACGAGA64600.033.36538321
TGCAGAA63950.033.3175131
AGACCTT64500.033.3124625
TTGCAGA64500.033.17293530
GAGACCT65350.032.913624
Produced by FastQC (version 0.11.2)