FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_27.34100000009a67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_27.34100000009a67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346540
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC52750.3917447680722444No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA15130.11236205385655088No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT13570.10077680573915368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5850.038.46128545
GGTAGCG301.14031915E-437.4983619
GCCCACG6550.034.34964418
TATGCCG6600.034.09068343
CCCACGA6650.033.83310719
ATGCCGT6700.033.5818744
CGTATGC7000.032.14145341
CCACGAG7250.031.65378820
AGCCCAC7150.031.46715517
CGAGACT7250.031.03312523
GTATGCC7450.030.20002242
ACGAGAC7500.029.9986922
CCTATCT7500.029.39871634
CCGAGCC7800.029.1333414
CGAGCCC7900.028.76456615
AGACTAG7900.027.62537625
CACGAGA8250.027.54424921
GACTAGA8050.027.39010426
TCGTATG8250.027.27153440
GAGCCCA8250.027.27153416
Produced by FastQC (version 0.11.2)