FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_26.34100000009a5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_26.34100000009a5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212724
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC23981.1272823000695738TruSeq Adapter, Index 12 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17630.8287734341212087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.5081702111656419No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10370.48748613226528276No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8160.3835956450612061No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7680.36103119535172334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.3102611835053873No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5490.25808089355220853No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5280.2482089468043098No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3980.18709689550779415No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3730.1753445779507719No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3620.17017355822568211No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3590.16876328011883943No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT3330.1565408698595363No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.14384836689795227No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2610.12269419529531223No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.11376243395197533No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2220.10436057990635753No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA3550.036.75979638
TCGTATG3550.036.75979640
TATCTCG800.036.56173736
CCCACGA3600.036.24924519
GTATGCC3600.036.24924542
ATGCCGT3600.036.24924544
TGCCGTC3600.036.24924545
CTCGTAT3650.035.75267839
CACGAGA3650.035.75267821
TATGCCG3650.035.75267843
TGCAATC2850.035.52557433
ATCTCGT3700.035.26953537
ACGAGAC3700.035.26953522
CCACGAG3700.035.26953520
GAGACTA3200.034.45240424
CGTATGC3800.034.3413941
CTACTTG2950.034.32131628
GCCCACG3900.034.0377518
CGAGACT3450.033.91233423
AGCCCAC3850.033.89539317