FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_25.34100000009a31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_25.34100000009a31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215667
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22011.0205548368549664No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA19900.9227188211455623No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13370.6199372180259382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.6069542396379604No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11570.536475214103224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.5063361571311327No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA9950.4613594105727811No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6600.30602734771661866No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC6510.301854247520483RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6030.27959771314109255No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5450.2527044007659957No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4500.20865500980678545No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4150.19242628682181326No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3930.1822253752312593No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.17109710804156408No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3460.16043251865143948No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.13817598427204902No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2670.12380197248535937No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2650.12287461688621812No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.11545577209308795No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA2440.11313738309523479No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT2280.10571853830210463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT2260.10479118270296336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC653.6379788E-1034.61413641
CTCGTAT901.2732926E-1129.99891939
TATCTCG751.6880222E-929.99891936
TTGCCCA300.005139005429.99891923
ATCTCGT803.3633114E-928.12398537
TGAGTAA406.151079E-428.12398534
CTATCTC654.84406E-727.69130935
TCGTATG856.4210326E-926.46963340
AGGAGCG450.001223582724.9990985
GACGAAT651.516729E-524.22989726
CGAGACG851.879489E-723.8226723
TCTCGTA952.0960215E-823.68335538
CGAATTG702.6906126E-522.4991928
TGCTATC702.6906126E-522.4991933
GAATTGC702.6906126E-522.4991929
ACGAATT702.6906126E-522.4991927
GAGTAAC500.002259167622.49918735
TATGCCG1003.6110578E-822.49918743
GTATGCC1003.6110578E-822.49918742
ATGCCGT1003.6110578E-822.49918744
Produced by FastQC (version 0.11.2)