FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_24.34100000009a24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_24.34100000009a24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308724
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC8860.2869877301408378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.18592658814993326No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3640.11790466565605524No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3460.11207421515657999No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG3450.11175030123994248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.11110247340666744No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC3280.10624376465710472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1450.037.23963539
TATGCCG1450.037.23963543
ATGCCGT1450.037.23963544
GCCCACG1500.035.99831418
GTATGCC1500.035.99831442
CGTATGC1550.034.8370841
CCCACGA1550.034.8370819
TCGTATG1650.034.08931440
GCGCCAT754.7293724E-1132.99845521
CTGGATA1650.032.7257430
GGATAAT1700.031.76322232
CCGAGCC1750.030.85569814
TTAGGGG300.005142672529.9985963
GTCGCGC751.6934791E-929.9985968
TCTCGTA1800.029.99859638
AGACCCT1800.029.99859625
CCTGGAT1800.029.99859629
AGCCCAC1850.029.18782417
GAGACCC1850.029.18782424
GGTCGCG852.0190782E-1029.1162877