FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_23.34100000009a17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_23.34100000009a17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593170
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC27420.4622620833825042No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG10170.17145169175784347No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC8740.14734393175649477No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA8090.136385859028609No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT7060.11902152839826693No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC6630.11177234182443482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4300.032.44289443
ATGCCGT4500.032.0010244
GTATGCC4450.031.3466742
CGTATGC4500.030.99837541
TCGTATG4500.030.99837540
GCCCACG4550.030.65773618
CTCGTAT4600.030.32449739
CCCACGA4600.030.32449719
TATCTCG4600.030.32449736
CGAGACA4600.029.83539223
CACGAGA4800.029.52970121
ACGAGAC5100.027.7926622
TACATCG654.8689253E-727.6908555
ATAATAT5100.026.91035332
TCTCGTA5200.026.82551638
TGCCGTC5400.026.66751745
TAATATC5500.026.18044533
CATAATA5350.026.07339931
ATCTCGT5450.025.59498837
CTCCGAG5650.025.0871812
Produced by FastQC (version 0.11.2)