FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_22.34100000009a0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_22.34100000009a0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065666
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTATAAGAGTGCTAATGTAAGTGACAGATATCTTTTTCACGCCATCATA36830.34560547113260626No Hit
AATTATGATGGCGTGAAAAAGATATCTGTCACTTACATTAGCACTCTTATA31860.29896796932622416No Hit
ATCCAAAGTTGCATATTCAATCTCTGACTTAACAGAATCAATCTCTGCTTG27760.26049437628675404No Hit
ACCTTAGGTGAAAGGTTAAAGAATCTGAATTGAACCTCAAGATGTGCAAAA27230.25552096060116397No Hit
TCATCATTCATACCATCAAATCCTAAAGCACACAACAACTGAAAAAACAAA23250.21817342394333683No Hit
CATTCATACCATCAAATCCTAAAGCACACAACAACTGAAAAAACAAACACC20430.19171109897472566No Hit
GTGATAGACTATCCAAATGAGTATTTGATATCAGTGGAGGGAACCTACAAC19430.1823272957943671No Hit
CTTCTCTACTCTCCAATGACTGTAAAACTTGCATTTTCTCTGAGTCACAAG19170.17988750696747385No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC18880.17716620404516986No Hit
GATCAAAACAAACCAACTTTCCTTCTCTAATCAAAACAAGAAAGACACAAA15900.14920247056770133No Hit
GTAGATATGAGAGCCTTAAAACAAGATGAAGTTCGTGACTATATTTCCATC15130.14197694211882522No Hit
ATCCATAGCTACAAAGTACCATCAGATGATTGTTTTGCACATCTTGAGGTT14880.13963099132373558No Hit
ATCATGATCAATGTGGTTCCAGTGACAAAAGAAGACGACAGAATCCATAGC13800.12949648388894833No Hit
ATCCTAAAGCACACAACAACTGAAAAAACAAACACCTTTCTATCTTCTTTG13350.12527377245778695No Hit
ATAGAAAGGTGTTTGTTTTTTCAGTTGTTGTGTGCTTTAGGATTTGATGGT13080.12274014559909015No Hit
CTGTAAAACTTGCATTTTCTCTGAGTCACAAGCAGAGATTGATTCTGTTAA12540.11767289188169651No Hit
GTTTTATAGCCTTTATAGTCTCGAATTACGAAGGAATTGGAGAGAAGTAAA11990.11251180013249931No Hit
AGGTTAAAGAATCTGAATTGAACCTCAAGATGTGCAAAACAATCATCTGAT11570.1085706027967487No Hit
CTCCAATGACTGTAAAACTTGCATTTTCTCTGAGTCACAAGCAGAGATTGA11470.10763222247871283No Hit
TCATTCATACCATCAAATCCTAAAGCACACAACAACTGAAAAAACAAACAC11340.10641232806526622No Hit
ATCTCTGACTTAACAGAATCAATCTCTGCTTGTGACTCAGAGAAAATGCAA10840.10172042647508694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2700.037.4999643
GCCCACG2700.037.498218
CCACGAG2750.036.81641420
TAGGTAA3000.036.7482385
CCCACGA2800.036.1589819
CCGAGCC2900.034.9121214
GTCTTAG3250.034.68371
TTAGGTA3250.033.921454
CACGAGA3000.033.74838321
GACTTGC2900.033.3604726
CGAGCCC3150.032.85556815
CGTATGC3100.032.65972541
AGCCCAC3200.032.34219717
ATCTCGT3100.031.93395237
TCTCGTA3100.031.93395238
TCCGAGC3250.031.84462713
TGCTGGA3100.031.20818130
TTGCTGG3250.030.4600829
GAGCCCA3350.030.22243116
AGACGTG300.00514829929.9985645
Produced by FastQC (version 0.11.2)