FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_21.341000000099fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_21.341000000099fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845608
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC26880.31787778734354455No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA10780.12748223763256733No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT9470.11199042582378596No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA9380.11092610287509105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3250.036.6932745
ATGCCGT3450.034.56612844
CCCACGA3500.034.0703119
GCCCACG3450.033.9119318
ACGACGC3500.033.42747527
CGACGCT3600.033.12391328
CGAGACG3550.032.95666523
GACGCTA3550.032.95666529
CGCTATA3500.032.78463731
TATGCCG3600.032.50085443
GTATGCC3600.032.49893642
CGTATGC3700.031.62058341
CTCGTAT3950.030.18888339
TATCTCG3950.030.18888336
ACGCTAT4050.029.44347830
TCGTATG4050.028.88794140
AGACGAC4250.027.52850725
CCACGAG4250.027.52850720
CACGAGA4400.027.10138121
TCTCGTA4400.027.10138138
Produced by FastQC (version 0.11.2)