FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_20.341000000099ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_20.341000000099ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688789
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC15910.23098510574355863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT2050.035.1205932
GCCCACG2200.033.74869518
ATGCCGT2350.032.55193344
CCCACGA2300.032.28135719
TATGCCG2250.031.9987643
TGCCGTC2350.031.59452245
CAAGGCG2300.031.30313529
GCGAATC2350.029.67970333
CGTATGC2600.028.55658741
CGGTCTA406.16501E-428.1239110
AGGCGAA2650.027.16875831
CGAGACC2650.027.16875823
AAGGCGA2650.027.16875830
CCGAGCC2750.026.99895514
GTATGCC2700.026.66563642
ACGAGAC2850.026.05162222
CTCGTAT2650.025.4707139
CTCCGAG3050.025.08099712
CGAGCCC3050.025.08099715
CACGAGA3100.024.67646421
Produced by FastQC (version 0.11.2)