FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_2.3410000000980c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_2.3410000000980c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323667
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.25798119672379327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.16436646306234495No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5200.16065894885793114No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5160.1594231107897932No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC5030.15540663706834495TruSeq Adapter, Index 12 (95% over 21bp)
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.1520080823809656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.13779594459737943No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4120.12729132101820698No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG3250.10041184303620695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG659.094947E-1238.07509243
GTCTAAA250.002083113936.0762981
CGTATGC702.0008883E-1135.35544241
CCTTATA401.5576305E-533.748382
ATGCCGT754.7293724E-1132.99841344
GCTGTAC300.005143068329.9985563
CTCGTAT852.0190782E-1029.11624739
ATATAAC406.158229E-428.1236483
CGAGACT803.3778633E-928.12364823
TCGCGCG654.857229E-727.6909759
GTATGCC903.929017E-1027.49867842
TGCAATC903.929017E-1027.49867833
GCCCACG957.366907E-1026.0513818
TCGTATG957.366907E-1026.0513840
TACTTGC901.1848897E-824.99879829
ACGAGAC1001.3369572E-924.7488122
GACTACT1001.3369572E-924.7488126
TGCCTAC651.494639E-524.2821241
CGCGCGC751.6920785E-623.99884810
CCCACGA1052.3519533E-923.57029519
Produced by FastQC (version 0.11.2)