FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_19.341000000099d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_19.341000000099d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895455
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC32810.366405905377713No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA10420.11636542316475981No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT9040.10095426347499316No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCGT301.1400037E-437.49841724
TGCCGTC4200.036.96272745
GCCCACG4700.033.50922818
ATGCCGT4650.032.90183644
TATGCCG4750.032.68283543
CCCACGA4950.031.8168419
CGAGACG5050.031.18680423
GTATGCC5000.031.04869342
CGTATGC5250.029.5701841
CCGAGCC5400.029.16543814
CCACGAG5600.028.92735120
AGCCCAC5500.028.63515517
TCGCGCG554.1649546E-628.6351559
TATCTCG5550.028.37718236
CGAGCCC5550.028.37718215
ACCGAGC406.166594E-428.1238148
ACCTATC5600.028.12381433
TCGTATG5650.027.47671740
CCTATCT5650.027.47671734
TAACCTA5800.027.15402631
Produced by FastQC (version 0.11.2)