FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_18.341000000099c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_18.341000000099c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809274
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC28830.35624522720364177RNA PCR Primer, Index 46 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3500.037.2863345
CGTATGC3600.036.8733341
GCCCACG3700.036.48483318
GCGCCTC250.002107209335.9983711
AGCCCAC3800.035.52470417
CGAGACC3800.035.52470423
TATGCCG3700.035.2708543
ATGCCGT3800.034.93478844
GTATGCC3950.034.17566742
CCACGAG4000.033.74847420
CCCACGA4100.032.9253419
CCGAGCC4050.032.77629514
GAGCCCA4200.032.141416
CGAGCCC4200.032.141415
ACGAGAC4350.031.03307522
TCGTATG4400.030.16908840
CATTCCG4450.029.8301129
TTCCGAA4400.029.65774731
TCCGAAT4450.029.32451632
CTCGTAT4500.028.99868239
Produced by FastQC (version 0.11.2)