FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_17.341000000099b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_17.341000000099b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886392
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC17980.20284479101796948No Hit
GACTAGGATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGA17060.19246563597144378No Hit
GTTGTAAGATGGGATAACGCAGTATCATCTGTGTTATCTCTGTCCTGTGTT13100.14779014251031147No Hit
AGGATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGATACT10490.11834493091092879No Hit
GGATAGATAGGAGAGTTGTAACACAGGACAGAGATAACACAGATGATACTG9760.11010929701531602No Hit
ATATTTCATTGACTAGGATAGATAGGAGAGTTGTAACACAGGACAGAGATA9250.10435563497865503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2350.036.38170243
GCCCACG2400.035.6237518
ATGCCGT2400.035.6237544
TGCGACC2350.035.4242929
CGAGCCC2450.034.89673615
CCCACGA2500.034.19880319
GACTGCG2550.033.52823326
TGCCGTC2550.033.52823345
AGCCCAC2650.033.11204517
CGACCTA2550.032.64591231
TCCGAGC2650.032.2630213
CCGAGCC2650.032.2630214
GAGCCCA2650.032.2630216
ACGAGAC2650.032.2630222
GTACGGT352.8204403E-432.1417273
GCGACCT2600.032.01810530
CTGCGAC2600.032.01810528
CGAGACT2750.031.90797423
GTATGCC2700.031.66555442
GACCTAT2900.029.48172432
Produced by FastQC (version 0.11.2)