FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_16.341000000099ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_16.341000000099ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686263
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC234033.4102086226417567No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT85451.245149454363706No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA37580.5476034698067651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA27350.39853525543414114No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC23440.3415600141636661No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC23340.34010284686774606No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC20560.29959359604116786No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC19650.2863333736482952No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA19190.27963040408706286No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC19010.2770075029544067No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG18160.26462158093908605No Hit
AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA17430.2539842596788695No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC16620.24218120458191686No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA15840.23081529967374026No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA15580.22702666470434804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG15200.22148942897985174No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGA12760.18593454695940187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11940.17398577513285723No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG11710.17063429035224106No Hit
AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG11580.16873997286754494No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC10190.14848534745425587No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG10190.14848534745425587No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG9950.14498814594404769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCG9880.14396812883690363No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC9720.14163666116343152No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAACTGTCTC9640.14047092732669544No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA9390.13682800908689524No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAACTGTCT9200.1340593912246471No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG8820.1285221555001508No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8670.12633640455627068No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTAT8640.1258992543674947No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG8350.12167346920932645No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAACTGTC8320.12123631902055043No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAACTGTCT8270.1205077353725904No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA8100.11803055096952625No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA8080.11773911751034224No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC7950.11584480002564614No Hit
GTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAG7890.11497049964809411No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAACTGTCTCTTA7840.11424191600013404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGAG7560.11016184757155784No Hit
GTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGCC7560.11016184757155784No Hit
GTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCC7460.10870468027563776No Hit
AAAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG7440.10841324681645376No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA7340.10695607952053367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.10622749587257364No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAACTGTCTCT7040.10258457763277344No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTAT6920.10083597687766935No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGGC207.032586E-444.99832521
TGGGCGC207.032586E-444.99832512
TGCCGTC24250.043.3282845
ACCTCGA301.1396922E-437.49860824
TACTCGA301.1396922E-437.4986082
GGGCGCT250.002106903635.99865713
TTGGGCG250.002106903635.99865711
ATGCCGT33400.031.45840644
CGTTAAG300.00510472830.049241
TGACGAG300.005146766529.99888613
ATCACGT602.4173642E-729.99888612
GCACACG300.005146766529.99888616
TACGACA300.005146766529.99888637
CTTACGG300.005146766529.99888633
GGGTGGA453.4897028E-529.99888222
TCTCGTA35200.029.65798438
ATCTCGT35150.029.57215737
TATGCCG35550.029.55586243
GTATGCC35550.029.55586242
CTTGCAG34950.029.54825629
Produced by FastQC (version 0.11.2)