FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_15.3410000000999f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_15.3410000000999f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446694
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC22700.5081778577728825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13300.2977429739374158No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9540.21356902040322906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8850.198122204462115No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA8520.19073459683810393No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7920.17730258297626564No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7780.17416844640850335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.15558749389962703No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5040.11282891643944176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3850.036.23268543
GTATGCC3900.035.76816642
TCGTATG3900.035.76816640
ATGCCGT3950.035.31540344
CGTATGC4050.034.44341741
CTCGTAT4050.034.44341739
TCTCGTA4050.034.44341738
TATCTCG4000.034.31147836
CGAGACT3900.034.0374523
ATCTCGT4100.034.02337637
AGACTAG3800.033.74899325
GCCCACG4200.033.21329518
CCTATCT4000.033.1865134
GACTAGA3950.033.03698726
GAGACTA3900.032.88363624
CCCACGA4250.032.82254819
TAGATCC4000.032.62402729
ACGAGAC4500.031.99904622
TGCCGTC4350.031.55078145
TCCTATC4100.031.27955433