FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_14.34100000009982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_14.34100000009982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371499
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC7820.21049854777536414TruSeq Adapter, Index 12 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.13432068457788582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCCA352.8170735E-432.142189
TATGCCG1600.026.7181943
GCCCACG1650.025.90854818
CGTATGC1650.025.90854841
GTCTATA450.001218425125.0230541
TGCCTAC450.001218425125.0230541
GTATGCC1800.024.99947542
TCGCGCG651.5203934E-524.230269
CGCGCGC751.6928025E-623.99949610
TCGTATG1800.023.74950240
GCGCGCT1052.3537723E-923.57093611
CTCGTAT1900.022.49952939
GCCTACA500.00226202222.4995292
ATGCCGT1950.021.92261944
CCCACGA2000.021.37455219
CGAGACT1900.021.31534423
ACTGCGC654.231393E-420.7687958
TACTTGC1850.020.67524129
GTATACA550.0039106620.4734081
GGGCGCC550.003932076520.45411721
Produced by FastQC (version 0.11.2)