FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_13.34100000009968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_13.34100000009968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736977
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1400.033.7489318
CGTATGC1450.032.58517541
CGAGACG1650.028.63545623
TCGTATG1750.028.28481940
ATGCCGT1700.027.79323644
TATGCCG1850.026.7559143
CTCGTAT1850.025.53973439
CCCACGA1950.025.38381219
CCTAGCG450.001226363224.99920837
CCGCGAA450.001226363224.9992089
TATCTCG1950.024.23000336
GTATGCC1950.024.23000342
CGTCCCG751.696737E-623.99923925
CGCGCGC851.8923129E-723.82277310
CGGCAGG952.1134838E-823.68346235
GTATCAA9400.023.4901751
ACGAATT1850.023.10737827
ACGTTAG602.443178E-422.531391
GGTATCA3200.022.531391
TCGGCAG1003.641071E-822.49928934
Produced by FastQC (version 0.11.2)