##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_1_12.3410000000994f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24866 Sequences flagged as poor quality 0 Sequence length 51 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.86885707391619 38.0 38.0 38.0 32.0 38.0 2 36.87649802943778 38.0 38.0 38.0 32.0 38.0 3 36.909876940400544 38.0 38.0 38.0 32.0 38.0 4 37.006756213303305 38.0 38.0 38.0 32.0 38.0 5 37.044076248692996 38.0 38.0 38.0 32.0 38.0 6 38.98487895117832 40.0 40.0 40.0 38.0 40.0 7 38.9104801737312 40.0 38.0 40.0 38.0 40.0 8 38.88466178717928 40.0 38.0 40.0 38.0 40.0 9 38.935815973618595 40.0 38.0 40.0 38.0 40.0 10 38.90291964932036 40.0 38.0 40.0 38.0 40.0 11 38.84030402959865 40.0 38.0 40.0 38.0 40.0 12 38.88353575162873 40.0 38.0 40.0 38.0 40.0 13 38.871752593903324 40.0 38.0 40.0 38.0 40.0 14 38.81247486527789 40.0 38.0 40.0 38.0 40.0 15 38.86378991393871 40.0 38.0 40.0 38.0 40.0 16 38.863025818386554 40.0 38.0 40.0 38.0 40.0 17 38.87316013834151 40.0 38.0 40.0 38.0 40.0 18 38.874487251668945 40.0 38.0 40.0 38.0 40.0 19 38.88912571382611 40.0 38.0 40.0 38.0 40.0 20 38.847422182900345 40.0 38.0 40.0 38.0 40.0 21 38.85651089841551 40.0 38.0 40.0 38.0 40.0 22 38.89037239604279 40.0 38.0 40.0 38.0 40.0 23 38.85876296951661 40.0 38.0 40.0 38.0 40.0 24 38.84155071181533 40.0 38.0 40.0 38.0 40.0 25 38.82622858521676 40.0 38.0 40.0 38.0 40.0 26 38.84122898737232 40.0 38.0 40.0 38.0 40.0 27 38.80334593420735 40.0 38.0 40.0 38.0 40.0 28 38.77925681653664 40.0 38.0 40.0 38.0 40.0 29 38.77036917879836 40.0 38.0 40.0 38.0 40.0 30 38.771334352127404 40.0 38.0 40.0 38.0 40.0 31 38.79763532534384 40.0 38.0 40.0 38.0 40.0 32 38.79313118314164 40.0 38.0 40.0 38.0 40.0 33 38.76421619882571 40.0 38.0 40.0 38.0 40.0 34 38.76522158771013 40.0 38.0 40.0 38.0 40.0 35 38.76132067883857 40.0 38.0 40.0 38.0 40.0 36 38.71370546127242 40.0 38.0 40.0 38.0 40.0 37 38.723236547896725 40.0 38.0 40.0 38.0 40.0 38 38.69219013914582 40.0 38.0 40.0 38.0 40.0 39 38.68125150808333 40.0 38.0 40.0 38.0 40.0 40 38.72609185232848 40.0 38.0 40.0 38.0 40.0 41 38.717566154588596 40.0 38.0 40.0 38.0 40.0 42 38.7076731279659 40.0 38.0 40.0 38.0 40.0 43 38.697940963564704 40.0 38.0 40.0 38.0 40.0 44 38.698343119118476 40.0 38.0 40.0 38.0 40.0 45 38.65748411485563 40.0 38.0 40.0 38.0 40.0 46 38.721426847904766 40.0 38.0 40.0 38.0 40.0 47 38.68249819030001 40.0 38.0 40.0 38.0 40.0 48 38.67980374808976 40.0 38.0 40.0 38.0 40.0 49 38.66178717928094 40.0 38.0 40.0 38.0 40.0 50 38.6911847502614 40.0 38.0 40.0 38.0 40.0 51 38.25645459663798 40.0 38.0 40.0 38.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.8633468462478291 2201 2 0.2451654347873813 2201 3 0.14460345218839876 2201 4 -0.6096579698425515 2201 5 -0.008054689501435064 2201 6 0.29928322476375513 2201 7 0.05895704626767895 2201 8 -0.508113315803719 2201 9 0.4544140662476295 2201 10 0.3393067523401072 2201 11 -0.9202527061324091 2201 12 0.4346816500743884 2201 13 0.20535188639188107 2201 14 -0.04005256250367495 2201 15 0.21451061259480753 2201 16 0.7209575551249188 2201 17 -0.4446505410237407 2201 18 0.09172511244533865 2201 19 0.6177281503828311 2201 20 -0.49720327498008743 2201 21 -0.5159985181790958 2201 22 -0.023842875744989556 2201 23 0.4666327154579477 2201 24 0.29948500041101767 2201 25 -0.23901904717127564 2201 26 0.2891554338665685 2201 27 0.3816457666607391 2201 28 0.4644653471405178 2201 29 0.47010328849489014 2201 30 -0.4492295812633458 2201 31 0.7314712477548753 2201 32 0.2709497407032373 2201 33 0.4846355801290869 2201 34 0.32779412118829754 2201 35 0.014775742519198332 2201 36 -0.5946845100782738 2201 37 -0.15911321074167262 2201 38 -0.6312915301171387 2201 39 -1.5280851391989572 2201 40 0.21903960064982897 2201 41 -0.9275335985396609 2201 42 0.019722332852225577 2201 43 -0.25665073669428295 2201 44 0.3088173906022291 2201 45 -0.20902402086397842 2201 46 -0.3452615831516255 2201 47 -0.2997191920587028 2201 48 0.030405977020983244 2201 49 -0.4262928935305865 2201 50 0.00342208608545036 2201 51 0.03256763296432297 1101 1 -0.5263590361051129 1101 2 0.03560661125796827 1101 3 0.5196034521883988 1101 4 -0.13906973454843552 1101 5 0.19231295755739097 1101 6 0.5198714600578711 1101 7 0.533221752150034 1101 8 -0.085319198156661 1101 9 0.05367877212998451 1101 10 0.35768910528128117 1101 11 0.9106296468087649 1101 12 0.24350517948615646 1101 13 0.43329306286246805 1101 14 -0.17240550368014596 1101 15 0.3027459067124525 1101 16 0.6327222610072738 1101 17 0.8586582825056723 1101 18 0.5384162889159256 1101 19 0.5294928562651862 1101 20 -0.22330621615655843 1101 21 0.6145161877032592 1101 22 0.6066718301373655 1101 23 -0.11424963748322625 1101 24 0.20022029452866263 1101 25 0.5789956587110794 1101 26 0.6347436691606845 1101 27 0.9349545901901521 1101 28 0.03983299419934383 1101 29 -0.43063200562275483 1101 30 -0.7047442871457008 1101 31 0.3748536006960492 1101 32 0.37756738776206333 1101 33 0.3522826389526159 1101 34 0.3792647094235946 1101 35 0.6103639778133143 1101 36 0.45494784286290013 1101 37 0.6625779657289144 1101 38 0.12973788164756428 1101 39 0.28441486080104283 1101 40 0.568304306532184 1101 41 0.2618046367544551 1101 42 -0.02807178479483241 1101 43 0.24886396918807208 1101 44 0.3088173906022291 1101 45 0.30016715560660856 1101 46 0.16209135802484553 1101 47 0.29770727852952916 1101 48 0.7234206829033383 1101 49 0.3825306358811815 1101 50 0.7001132625560373 1101 51 0.688817632964323 2202 1 -0.5949864870855066 2202 2 -0.12289012076817585 2202 3 -0.2859521033671584 2202 4 0.3903420301574485 2202 5 -0.0740269117236565 2202 6 0.3270610025415337 2202 7 -0.1840985092878782 2202 8 -0.4039466491370547 2202 9 0.058580732914293776 2202 10 -0.9593043587710071 2202 11 -0.15636381724352333 2202 12 -0.7597627943700545 2202 13 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0.5459293286916349 2202 50 0.8471720860854504 2202 51 -0.8667379225912342 1102 1 -0.4473674394664542 1102 2 -0.13162027949833544 1102 3 0.5338891664741112 1102 4 -0.1096579698425515 1102 5 -0.8160904037871504 1102 6 -1.779288203807674 1102 7 -1.2499715251608947 1102 8 -0.020613315803721832 1102 9 0.056199780533340515 1102 10 -0.676764676231322 1102 11 -0.3952527061324105 1102 12 0.013253078645817595 1102 13 -0.3196481136081175 1102 14 0.267090294639182 1102 15 -0.3854893874051939 1102 16 0.8781004122677771 1102 17 0.011599458976256471 1102 18 -1.0145248875546642 1102 19 -1.1679861353314536 1102 20 -0.240953274980086 1102 21 -0.14010566103623745 1102 22 0.023478552826439625 1102 23 0.32020414402937547 1102 24 -0.45944357101755173 1102 25 0.3386595242572952 1102 26 0.2784411481522824 1102 27 0.43075290951788503 1102 28 -0.26857036714519467 1102 29 -0.4709681400765362 1102 30 -0.13583672412048742 1102 31 -0.28817160938798025 1102 32 0.026306883560380356 1102 33 -0.3367929912994825 1102 34 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1213 32 -0.3295937375576301 1213 33 -0.874060072044827 1213 34 -0.9711189222899606 1213 35 -0.46891990965471564 1213 36 -0.5770214666000157 1213 37 -0.059928428132977274 1213 38 -0.8024871822910526 1213 39 -0.7155851391989572 1213 40 -1.060851703697999 1213 41 -0.5783488159309655 1213 42 -0.9232124497564698 1213 43 0.1197079589578891 1213 44 -1.1477043485282081 1213 45 -0.748426194777025 1213 46 -0.5463485396733674 1213 47 -0.36086049640653073 1213 48 -0.3839961968920633 1213 49 -0.9589015891827586 1213 50 -1.6963061747841124 1213 51 -0.5503128018182863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 2.0 22 3.0 23 9.0 24 17.0 25 30.0 26 47.0 27 76.0 28 110.0 29 127.0 30 176.0 31 208.0 32 301.0 33 343.0 34 439.0 35 559.0 36 830.0 37 1350.0 38 2854.0 39 17383.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.83217149534593 15.912479348833461 9.880323971471169 29.37502518434944 2 18.977720582321243 19.874527467224322 33.86954073835759 27.278211212096835 3 19.942893911364916 21.15740368374487 28.943135204697178 29.956567200193035 4 15.117027266146547 14.39716882490147 35.29317139869701 35.19263251025497 5 15.96557548459744 34.25561006997507 33.913777849272094 15.865036596155393 6 34.163114292608384 35.586744952947804 15.016488377704496 15.233652376739323 7 30.35067964288587 30.809136974181612 19.387919247164803 19.452264135767717 8 27.33853454516207 33.07729429743424 19.15466902597925 20.429502131424435 9 26.731279658972092 13.645137939354942 18.58762969516609 41.035952706506876 10 16.987050591168664 24.768760556583285 30.563822086383013 27.68036676586504 11 38.14847583045122 20.76329124105204 21.752593903321802 19.335639025174938 12 23.05155634199308 22.745918121129254 29.10801898174214 25.094506555135528 13 30.294377865358317 18.635888361618274 25.64948121933564 25.420252553687767 14 22.951017453551035 17.93613769806161 24.081074559639667 35.03177028874769 15 26.791602992037323 25.090484999597845 21.47912812675943 26.638783881605406 16 25.46448966460227 26.200434327998067 22.520711011019063 25.8143649963806 17 24.849191667336925 25.34384299847181 24.34649722512668 25.46046810906459 18 26.156197217083566 25.227217887879032 24.53148877986005 24.08509611517735 19 26.381404327193756 25.02614011099493 23.53414300651492 25.058312555296386 20 25.573071664119684 24.169548781468674 24.330411002975953 25.926968551435696 21 26.69106410359527 22.890694120485804 24.563661224161507 25.854580551757422 22 25.33177833185876 23.900104560443978 23.96042789350921 26.807689214188045 23 26.42564143810826 24.14541944824258 23.337086785168502 26.09185232848066 24 25.846537440682056 24.61594144615137 23.71511300571061 25.822408107455963 25 25.520791442129813 24.097160781790397 24.487251668945547 25.894796107134237 26 25.351886109547173 24.410842113729593 23.952384782433846 26.28488699428939 27 26.26880077213866 24.06900989302662 24.173570337006353 25.48861899782836 28 25.279498109868896 24.06498833748894 24.563661224161507 26.09185232848066 29 25.44840344245154 24.648113890452827 23.542186117590283 26.361296549505347 30 25.046247888683343 24.603876779538325 24.499316335558593 25.850558996219736 31 25.396123220461675 23.33306522963082 24.829083889648516 26.44172766025899 32 24.91353655593984 24.16552722593099 23.972492560122255 26.948443658006916 33 24.845170111799245 24.398777447116547 25.203088554652943 25.552963886431275 34 25.66958899702405 23.550229228665646 24.720501890131104 26.0596798841792 35 25.295584332019626 24.398777447116547 23.916190782594708 26.38944743826912 36 25.77414944100378 23.95640633797153 24.354540336202042 25.91490388482265 37 27.089198101825783 23.79554411646425 23.007319231078583 26.10793855063138 38 25.19906699911526 23.8960830049063 24.607898335076005 26.296951660902433 39 26.272822327676344 24.113247003941122 24.35051878066436 25.263411887718167 40 25.074398777447115 23.916190782594708 24.808976111960106 26.200434327998067 41 25.420252553687767 23.835759671841068 25.705782996863185 25.03820477760798 42 25.484597442290678 23.256655674414866 24.93766588916593 26.321080994128526 43 26.039572106490787 23.4939274511381 24.74865277889488 25.717847663476235 44 24.953752111316657 23.385345451620687 25.235260998954395 26.42564143810826 45 24.909515000402155 23.952384782433846 25.36395077616022 25.77414944100378 46 25.235260998954395 23.41349634038446 25.573071664119684 25.778170996541462 47 24.724523445668783 24.008686559961394 25.26743344325585 25.999356551113973 48 24.3867127805035 23.562293895278692 26.204455883535754 25.846537440682056 49 24.435954152423083 23.185200080434345 26.587572893625577 25.79127287351699 50 24.02171727327569 24.02976070782224 25.819424894429922 26.12909712447215 51 24.398777447116547 24.093139226252713 25.28754122094426 26.22054210568648 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 2.0 15 4.0 16 6.0 17 4.0 18 2.0 19 2.5 20 3.0 21 3.0 22 3.0 23 2.5 24 2.0 25 1.5 26 4.0 27 7.0 28 20.5 29 34.0 30 47.0 31 60.0 32 96.0 33 132.0 34 189.0 35 246.0 36 374.0 37 502.0 38 655.5 39 809.0 40 1008.0 41 1207.0 42 1440.5 43 1674.0 44 1867.5 45 2061.0 46 2171.5 47 2282.0 48 2366.5 49 2451.0 50 2501.0 51 2551.0 52 2447.0 53 2343.0 54 2060.5 55 1778.0 56 1598.0 57 1418.0 58 1287.5 59 1157.0 60 1019.5 61 882.0 62 756.0 63 630.0 64 597.0 65 564.0 66 600.5 67 637.0 68 536.5 69 436.0 70 392.5 71 349.0 72 346.0 73 343.0 74 253.0 75 107.5 76 52.0 77 51.0 78 50.0 79 35.0 80 20.0 81 13.0 82 6.0 83 4.5 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19705622134641682 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.004021555537681976 50 0.004021555537681976 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 24866.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.7225483519327 #Duplication Level Percentage of deduplicated Percentage of total 1 94.00383562497139 81.52252180253714 2 3.494601203095142 6.061214436122813 3 0.8999544759137449 2.3413903665590396 4 0.5524201086491787 1.9162911833163327 5 0.24624957873943054 1.0677695499436661 6 0.1452856641818752 0.7559725822113196 7 0.15100924259718596 0.9167134439906237 8 0.10049118727880293 0.6971881478183289 9 0.05204174397070706 0.406187339203669 >10 0.3494773689956972 4.061582445438574 >50 0.004633801606838888 0.2531687028584798 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG 63 0.2533579988739645 No Hit CTTGTGGAGACGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTAACGG 25 0.10053888844204939 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.004021555537681976 26 0.0 0.0 0.004021555537681976 27 0.0 0.0 0.008043111075363952 28 0.0 0.0 0.016086222150727903 29 0.0 0.0 0.09651733290436741 30 0.0 0.0 0.17292688812032495 31 0.0 0.0 0.2975951097884662 32 0.0 0.0 0.3900908871551516 33 0.0 0.0 0.4705219979087911 34 0.0 0.0 0.5831255529638865 35 0.0 0.0 0.7158368857073916 36 0.0 0.0 0.9008284404407625 37 0.0 0.0 1.2426606611437303 38 0.0 0.0 1.6930748813641117 39 0.0 0.0 2.115338212820719 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGCAC 30 0.0050542676 29.997316 34 >>END_MODULE