FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_11.34100000009925.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_11.34100000009925.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461427
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAC300.005144890429.999063
TATATCG507.150795E-526.9991575
TTATACT500.002262982822.4992964
TATATAC602.464927E-422.4992943
ACGGGAC754.5960765E-520.99934216
GTATCAA6550.020.9831031
CTGGACT654.2338282E-420.7685814
GTATATA550.003901739620.482761
TCAATAC1109.954965E-820.4539053
GTGTTAG706.910383E-419.3123171
AACGCAG6800.018.8597036
CCTATAC600.00650590418.7494132
TCGCGCG600.00650590418.7494139
CATGCAG750.001109087517.9994355
TAGACCG750.001109087517.9994355
CAGTGTA750.001109087517.9994359
ATACCCG750.001109087517.9994355
ACGCAGA7250.017.68917
TATCAAC7450.017.5162322
CGCAGAG7250.017.3787658
Produced by FastQC (version 0.11.2)