FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_10.3410000000990b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_10.3410000000990b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365858
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG4530.12381853068676918No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC3850.105232084579263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG301.1387473E-437.49806218
CTATTAG401.5379193E-533.8129541
ATAGGGC352.8174982E-432.1411973
ATCGCCA554.1564126E-628.63488637
CGCGCGA507.147805E-526.99860610
GTCGCGC1058.367351E-1125.712968
CGCGCGC1058.367351E-1125.7129610
AGTAGGA801.0502117E-725.31119541
TAGGGTG450.001225325324.9987095
TCGCGCG1101.5097612E-1024.5441889
GGGGCAG651.520673E-524.22951918
CGGGGCA1400.024.10589817
GTGTATA802.9179482E-622.541971
TATGCCG500.002260564622.50191343
TCGCCAG702.6975682E-522.49883838
CCCACGA500.00226236122.49883819
GTATGCC500.00226236122.49883842
GCCTCGA1003.6292477E-822.49883832
CGTCGGG1003.6292477E-822.49883814
GCGACCT602.4639122E-422.49883713
Produced by FastQC (version 0.11.2)