##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_1_1.341000000097d4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 67180 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.94511759452218 38.0 38.0 38.0 32.0 38.0 2 36.92749330157785 38.0 38.0 38.0 32.0 38.0 3 36.93733253944627 38.0 38.0 38.0 32.0 38.0 4 36.99124739505805 38.0 38.0 38.0 32.0 38.0 5 36.99435844001191 38.0 38.0 38.0 32.0 38.0 6 38.962935397439715 40.0 38.0 40.0 38.0 40.0 7 38.91714796070259 40.0 38.0 40.0 38.0 40.0 8 38.85486752009527 40.0 38.0 40.0 38.0 40.0 9 38.855924382256624 40.0 38.0 40.0 38.0 40.0 10 38.81623995236678 40.0 38.0 40.0 38.0 40.0 11 38.8055075915451 40.0 38.0 40.0 38.0 40.0 12 38.820169693361116 40.0 38.0 40.0 38.0 40.0 13 38.787570705567134 40.0 38.0 40.0 38.0 40.0 14 38.76747543911878 40.0 38.0 40.0 38.0 40.0 15 38.83326883000893 40.0 38.0 40.0 38.0 40.0 16 38.85244120273891 40.0 38.0 40.0 38.0 40.0 17 38.84678475736826 40.0 38.0 40.0 38.0 40.0 18 38.862831199761835 40.0 38.0 40.0 38.0 40.0 19 38.87631735635606 40.0 38.0 40.0 38.0 40.0 20 38.846650788925274 40.0 38.0 40.0 38.0 40.0 21 38.89127716582316 40.0 38.0 40.0 38.0 40.0 22 38.85433164632331 40.0 38.0 40.0 38.0 40.0 23 38.87773146769872 40.0 38.0 40.0 38.0 40.0 24 38.871792200059545 40.0 38.0 40.0 38.0 40.0 25 38.85230723429592 40.0 38.0 40.0 38.0 40.0 26 38.82366775826139 40.0 38.0 40.0 38.0 40.0 27 38.78682643643942 40.0 38.0 40.0 38.0 40.0 28 38.744462637689786 40.0 38.0 40.0 38.0 40.0 29 38.69184281036023 40.0 38.0 40.0 38.0 40.0 30 38.64261685025305 40.0 38.0 40.0 38.0 40.0 31 38.57674903245013 40.0 38.0 40.0 38.0 40.0 32 38.47914557904138 40.0 38.0 40.0 38.0 40.0 33 38.393286692467996 40.0 38.0 40.0 38.0 40.0 34 38.299434355462935 40.0 38.0 40.0 38.0 40.0 35 38.2089312295326 40.0 38.0 40.0 38.0 40.0 36 38.03789818398333 40.0 38.0 40.0 38.0 40.0 37 37.968234593629056 40.0 38.0 40.0 38.0 40.0 38 37.84940458469783 40.0 38.0 40.0 32.0 40.0 39 37.71638880619232 40.0 38.0 40.0 32.0 40.0 40 37.58862756772849 40.0 38.0 40.0 32.0 40.0 41 37.45123548675201 40.0 38.0 40.0 32.0 40.0 42 37.290532896695446 40.0 38.0 40.0 32.0 40.0 43 37.10846978267342 40.0 38.0 40.0 32.0 40.0 44 36.85007442691277 40.0 38.0 40.0 27.0 40.0 45 36.594656147662995 40.0 38.0 40.0 27.0 40.0 46 36.366686513843405 40.0 38.0 40.0 27.0 40.0 47 36.08943137838642 40.0 38.0 40.0 13.0 40.0 48 35.80851443882108 40.0 38.0 40.0 13.0 40.0 49 35.51044953855314 40.0 38.0 40.0 13.0 40.0 50 35.31120869306341 40.0 38.0 40.0 13.0 40.0 51 34.779205120571596 40.0 38.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.32528320550687795 2201 2 0.03952294732469852 2201 3 -0.02652876205790733 2201 4 0.3237295161780267 2201 5 0.23659778640449503 2201 6 0.2723320891033012 2201 7 0.3562608324964103 2201 8 0.3380407192745878 2201 9 -0.004353211558317582 2201 10 0.1804590886521069 2201 11 -0.16749601467086705 2201 12 -0.1585441222937618 2201 13 0.0177605251659827 2201 14 0.41673463625973284 2201 15 0.4249399681364707 2201 16 0.11441880572750307 2201 17 0.1860036346129803 2201 18 0.49571856538925374 2201 19 -0.0692981005168889 2201 20 0.10686149106035003 2201 21 0.12164021964191107 2201 22 0.3491924289007784 2201 23 0.3525509165609151 2201 24 0.04440190469387062 2201 25 -0.38895195214170997 2201 26 -0.5285747467705875 2201 27 -0.5567090958591194 2201 28 -0.3791945501612659 2201 29 0.08302755268875472 2201 30 0.10376340216498647 2201 31 -0.2505136112645303 2201 32 -0.38690348181911816 2201 33 -0.6341678144712617 2201 34 -0.1325297504104057 2201 35 -0.12272278375680656 2201 36 -0.07778435675270856 2201 37 0.5373964596601937 2201 38 -0.0889251760479084 2201 39 -0.15445372136184687 2201 40 0.17962477022800982 2201 41 0.30547418277389937 2201 42 0.02106376631257234 2201 43 0.1869754662186196 2201 44 0.3285057582305271 2201 45 1.074695832678053 2201 46 -0.29137558600240965 2201 47 -0.039325830135901185 2201 48 -0.2697398683313281 2201 49 0.6841631656871243 2201 50 0.70753791192665 2201 51 0.43297299880115503 1101 1 -0.35711379823843714 1101 2 0.3418949822810049 1101 3 -0.13988706418025743 1101 4 -0.4028622441216001 1101 5 0.3855366128714124 1101 6 0.5330062464066714 1101 7 0.5954618312479667 1101 8 -0.0306234505131755 1101 9 0.35357437895354593 1101 10 0.6636051560678382 1101 11 0.70453894163375 1101 12 0.7927667391294619 1101 13 0.5207567798476305 1101 14 0.4525648484944398 1101 15 0.28161911919764293 1101 16 -0.20043762373566665 1101 17 0.45616593174157316 1101 18 0.28623042556403533 1101 19 0.35567068849684347 1101 20 0.567535648363723 1101 21 0.27944296620870546 1101 22 0.29538468857618483 1101 23 0.187132689344935 1101 24 0.29034447647914163 1101 25 -0.035269055762178425 1101 26 0.38528293113203205 1101 27 0.41207991787371867 1101 28 0.3553872226227526 1101 29 0.0911424091182198 1101 30 0.3703052248865859 1101 31 0.2699607957267318 1101 32 0.7602875294168356 1101 33 0.6106511618083843 1101 34 0.2634752433473935 1101 35 0.6878889515740312 1101 36 0.8887574659688866 1101 37 0.768482601982285 1101 38 0.6060810661493434 1101 39 0.7408022087255475 1101 40 0.897477454372833 1101 41 0.9790072664193445 1101 42 0.9149464130042091 1101 43 0.654016664720487 1101 44 0.5352473312642374 1101 45 0.7590903395444712 1101 46 0.2521949508265493 1101 47 0.716729101199924 1101 48 0.9742052003328467 1101 49 0.5080083591952373 1101 50 0.3501096971950659 1101 51 0.8303512759547118 2202 1 0.44498017520384536 2202 2 0.38977547257722023 2202 3 0.3237237631946144 2202 4 0.25175981920832413 2202 5 0.10604223084894215 2202 6 0.09505936183057173 2202 7 0.24919012542569874 2202 8 0.47869728493115105 2202 9 -0.29172694893205176 2202 10 0.47591363410665366 2202 11 0.09639287421801868 2202 12 0.061152847403207033 2202 13 0.39882113122659035 2202 14 0.364209383734476 2202 15 -0.04677720358070303 2202 16 -0.045177153868451114 2202 17 0.4256500992594425 2202 18 -0.0643824447117538 2202 19 -0.3119748681936585 2202 20 0.06140694560580329 2202 21 0.15674122974292004 2202 22 -0.38464595493760356 2202 23 -0.1184086793986836 2202 24 0.07596756125953164 2202 25 0.042361179171422236 2202 26 -0.2482717164675563 2202 27 0.13015959100956564 2202 28 0.48166403569732097 2202 29 0.20171442137562678 2202 30 0.25654117994276504 2202 31 0.3249914392405202 2202 32 -0.20281257272821307 2202 33 0.5314887511853001 2202 34 -0.047428740309399586 2202 35 0.46010549907147436 2202 36 0.024993421025065743 2202 37 0.7320934293571568 2202 38 0.2373374502147172 2202 39 0.6564048644967428 2202 40 0.5180086086118507 2202 41 0.37239337469308964 2202 42 0.9314173016661087 2202 43 0.38167243591558275 2202 44 0.41714212186689537 2202 45 0.447675630657848 2202 46 0.13766481803799024 2202 47 0.10360346279338728 2202 48 0.43960356601211004 2202 49 0.23694094346490147 2202 50 0.10955811394685355 2202 51 0.39585178667994114 1102 1 -0.3997167944931235 1102 2 0.013828502880251392 1102 3 0.20819346016431695 1102 4 0.177896182844691 1102 5 -0.018957769151057846 1102 6 -1.031834577563366 1102 7 -0.41179472305914544 1102 8 -0.09043150294763791 1102 9 -0.3078254337805362 1102 10 -0.007040911347893086 1102 11 -0.8202737924486456 1102 12 -0.0856274556270904 1102 13 0.04276052516598128 1102 14 -0.07423758596249286 1102 15 -0.9028378096413121 1102 16 -0.20710897205027123 1102 17 -0.03552414316479968 1102 18 0.10891300983369945 1102 19 0.05639634392755255 1102 20 -0.17438850893964997 1102 21 -0.7040542248025332 1102 22 0.05474798445633411 1102 23 0.16852313878313652 1102 24 0.09301301580498489 1102 25 -0.15839639658614857 1102 26 0.5130919198960768 1102 27 0.45023534858532344 1102 28 -0.2465556612723816 1102 29 0.2774719971331976 1102 30 -0.28512548672389926 1102 31 -0.5310691668200889 1102 32 -0.09107014848578388 1102 33 0.2644432966398469 1102 34 0.40427580514514716 1102 35 0.20158277179874773 1102 36 0.23332675435840144 1102 37 0.5644797929935237 1102 38 0.2117692683965373 1102 39 0.292768500860376 1102 40 -0.0946807853275402 1102 41 -0.1702202616705435 1102 42 -0.4824084559096491 1102 43 0.6411421328852853 1102 44 0.7826724248971928 1102 45 0.8302513882336058 1102 46 0.48709663621981036 1102 47 0.9426186143085431 1102 48 0.2795656872242276 1102 49 0.018190943464901466 1102 50 -0.49176764362890424 1102 51 0.2225563321344879 2203 1 -0.1636056833820092 2203 2 -0.07360836580661356 2203 3 0.03205709652795008 2203 4 -0.16111896867045772 2203 5 -0.24117999137327928 2203 6 -0.2205972038259958 2203 7 0.1178769941125708 2203 8 0.45218213341600233 2203 9 0.1703942631891593 2203 10 -0.03166212346910413 2203 11 -0.07153641871127547 2203 12 -0.2595542233038586 2203 13 -0.22365361624815705 2203 14 0.3288558483809467 2203 15 -0.0013226581261562842 2203 16 0.035630926939624885 2203 17 0.3435793921887367 2203 18 0.13006199973268906 2203 19 0.07817664695785709 2203 20 -0.3577849735861136 2203 21 0.07719577519746679 2203 22 -0.07403989433154123 2203 23 0.12527818928818846 2203 24 0.28682614711811283 2203 25 0.26458340139364367 2203 26 0.44112222292638137 2203 27 -0.12236566151568695 2203 28 -0.31252788349460303 2203 29 0.1789871486483534 2203 30 -0.0831052847036986 2203 31 0.3060520453011222 2203 32 0.3615813666657317 2203 33 -0.1907334710369213 2203 34 -0.1547519726326314 2203 35 -0.13585409688812433 2203 36 0.22322574425739106 2203 37 -0.625229802966075 2203 38 -0.3687231558458848 2203 39 -0.02415069105881429 2203 40 -0.3860317954285506 2203 41 0.21961559691531818 2203 42 -0.24156249631369064 2203 43 -0.40999423075108155 2203 44 -1.3038174740927033 2203 45 -0.8667183087360897 2203 46 -0.9014765961034215 2203 47 -0.7120531028631731 2203 48 -1.1121641107555646 2203 49 -1.7996752181512576 2203 50 -1.6126641082753679 2203 51 -0.7114714456432907 1103 1 0.21861653884020882 1103 2 0.0484118362135888 1103 3 0.3623601268309784 1103 4 0.5103961828446941 1103 5 0.32104223084894556 1103 6 0.012332089103296084 1103 7 0.19737194360752142 1103 8 0.18915183038569694 1103 9 0.350091232886129 1103 10 -0.0795409113478911 1103 11 -0.04860712578198445 1103 12 0.4147892110395759 1103 13 0.5510938584993141 1103 14 0.07784574737084426 1103 15 0.3704955236920213 1103 16 0.2666410279497313 1103 17 -0.048440809831461706 1103 18 0.5101630098336969 1103 19 0.28847967726088797 1103 20 0.46686149106034946 1103 21 0.09719577519746991 1103 22 0.07808131778966754 1103 23 0.3047731387831334 1103 24 0.38551301580498176 1103 25 0.30327027008051743 1103 26 0.6980919198960791 1103 27 0.04106868191865942 1103 28 -0.06697232793904817 1103 29 0.280805330466535 1103 30 0.6915411799427673 1103 31 0.3172641665132474 1103 32 0.5330965181808835 1103 33 0.7869432966398477 1103 34 0.6530258051451483 1103 35 -0.0371672282012554 1103 36 -0.16000657897493653 1103 37 0.6707297929935265 1103 38 0.6155192683965396 1103 39 1.0277685008603754 1103 40 0.8507358813391264 1103 41 0.8910297383294576 1103 42 0.7955082107570135 1103 43 0.2503087995519522 1103 44 0.01850575823053191 1103 45 0.14358472156693836 1103 46 0.8608466362198115 1103 47 0.6917852809752105 1103 48 0.5391490205575664 1103 49 0.07194094346490232 1103 50 0.10864902303776347 1103 51 0.846306332134489 2204 1 0.29162624757807976 2204 2 -0.03663670747573633 2204 3 0.37303973848146654 2204 4 0.2913670566310955 2204 5 0.09754708521787592 2204 6 0.28592432211300434 2204 7 0.4265952445783938 2204 8 0.07148192747307291 2204 9 0.217761135798753 2204 10 0.3260901566132688 2204 11 0.318480252858798 2204 12 0.5728474634667577 2204 13 0.4938123051012582 2204 14 0.36376807746793105 2204 15 0.15136931009978838 2204 16 0.06867986290118466 2204 17 0.3163164717219331 2204 18 0.30521155352302287 2204 19 0.5162466675521529 2204 20 0.4952110056234602 2204 21 0.18399189170231978 2204 22 0.4099259779838462 2204 23 0.3854527504336218 2204 24 0.14580427794090411 2204 25 0.5022023089154644 2204 26 0.23517929853685615 2204 27 -0.09931966759590694 2204 28 0.4080762157502775 2204 29 0.2540092139616803 2204 30 0.5757159372243166 2204 31 0.5923127102025703 2204 32 0.7558149647828216 2204 33 1.1691763063485823 2204 34 0.23467629058203698 2204 35 0.257104616458939 2204 36 0.1912555569473966 2204 37 0.42704047260517086 2204 38 0.4147425693674123 2204 39 0.48815685037494205 2204 40 0.649959182309999 2204 41 0.9435540101741182 2204 42 0.1257023855142947 2204 43 0.4552602558626262 2204 44 -0.2074165718665597 2204 45 -0.19563857940393348 2204 46 0.36968158767611925 2204 47 0.5261542130140455 2204 48 0.6793431953148428 2204 49 1.1386399725911147 2204 50 0.27593057643581886 2204 51 0.2993160408723554 2205 1 -0.26213817814092266 2205 2 -0.31498439020150926 2205 3 0.01519031551022465 2205 4 0.3771886356748766 2205 5 0.131985627075359 2205 6 0.11950190042405495 2205 7 -0.0552695658264426 2205 8 -0.3697160941426034 2205 9 0.12122330835782691 2205 10 0.44461003204833105 2205 11 0.11761928931235843 2205 12 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-0.17093362407053547 1213 49 -0.1256623623202202 1213 50 -0.423499737292822 1213 51 0.1489509602336625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 4.0 16 5.0 17 7.0 18 1.0 19 4.0 20 0.0 21 9.0 22 19.0 23 37.0 24 64.0 25 131.0 26 196.0 27 329.0 28 425.0 29 668.0 30 964.0 31 1157.0 32 1440.0 33 1748.0 34 2293.0 35 2542.0 36 3280.0 37 4574.0 38 7693.0 39 39590.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.811427634423026 16.500454660644277 12.366769524320617 21.321348180612077 2 16.911283119976183 21.530217326585294 39.130693658827035 22.42780589461149 3 21.84727597499256 27.843108067877342 27.479904733551653 22.829711223578446 4 13.04852634712712 15.879726108961002 36.34117296814528 34.730574575766596 5 16.576362012503722 35.8841917237273 30.09377791009229 17.445668353676687 6 30.296219112831203 36.27270020839536 14.767788032152426 18.66329264662102 7 28.889550461446863 31.944030961595715 20.26347127121167 18.90294730574576 8 28.983328371539148 30.15927359333135 18.16612086930634 22.691277165823163 9 28.86722238761536 14.543018755582018 20.83209288478714 35.75766597201548 10 21.309913664781185 24.94343554629354 26.848764513247993 26.897886275677287 11 33.14081571896398 24.016076213158676 22.843108067877342 20.0 12 27.030366180410837 24.782673414706757 28.434057755284307 19.752902649598095 13 30.681750520988388 22.213456385829115 26.11193807680857 20.99285501637392 14 23.506996129800537 20.092289371836856 30.45400416790712 25.946710330455492 15 23.983328371539148 26.1938076808574 27.222387615361715 22.60047633224174 16 21.300982435248585 28.529324203632033 27.17326585293242 22.996427508186958 17 21.31289074129205 25.547782077999404 27.162846085144388 25.976481095564157 18 23.94164930038702 23.591842810360227 30.09675498660316 22.369752902649598 19 26.332241738612684 23.328371539148556 30.570110151830903 19.76927657040786 20 24.093480202441203 22.506698422149448 30.464423935695145 22.9353974397142 21 24.62488835963084 23.426615064007144 31.15510568621613 20.793390890145876 22 22.10330455492706 24.431378386424534 31.09556415599881 22.369752902649598 23 21.836856207204526 26.122357844596607 32.067579636796665 19.9732063114022 24 21.976778803215243 25.86484072640667 31.854718666269722 20.303661804108366 25 20.497171777314676 27.100327478416197 32.45906519797559 19.94343554629354 26 20.10270913962489 27.907115212860973 31.752009526644837 20.238166120869305 27 20.80976481095564 27.718070854420958 31.254837749330157 20.21732658529324 28 20.08335814230426 28.03512950282822 30.71896397737422 21.1625483774933 29 20.153319440309616 28.782375707055674 29.970229234891338 21.09407561774338 30 20.005954153021733 28.426615064007144 30.619231914260197 20.948198868710925 31 20.333432569217027 27.549866031557013 30.483774933015777 21.632926466210183 32 21.016671628460852 27.658529324203634 30.08931229532599 21.23548675200953 33 20.741292051205715 28.408752604941945 30.308127418874665 20.54182792497767 34 20.500148853825543 28.402798451920212 29.545995832092885 21.551056862161357 35 20.669842214944925 29.202143495087824 29.73652872878833 20.391485561178925 36 20.556713307532004 29.554927061625484 29.72759749925573 20.160762131586782 37 20.547782077999404 30.242631735635605 29.164930038701996 20.044656147662995 38 19.7082465019351 30.136945519499854 29.735040190532896 20.419767788032154 39 19.86305448050015 30.169693361119382 29.36588270318547 20.601369455194998 40 20.008931229532596 30.311104495385532 29.25870794879428 20.421256326287583 41 20.058052991961894 29.947901161059836 28.853825543316464 21.140220303661806 42 20.669842214944925 29.781184876451324 28.030663888061923 21.518309020541828 43 19.82435248585889 29.557904138136347 28.198868710925872 22.418874665078892 44 19.57725513545698 29.64870497171777 28.559094968740695 22.21494492408455 45 20.209883894016077 29.61298005358738 27.408454897290863 22.768681155105686 46 20.654956832390592 28.807680857398033 27.76719261685025 22.77016969336112 47 20.053587377195594 28.67817802917535 27.32807383149747 23.940160762131587 48 19.654659124739506 28.34176838344745 27.024412027389104 24.979160464423934 49 20.058650153324002 27.909434636339274 27.339307511387656 24.69260769894906 50 19.81542125632629 27.81333730276868 27.448645430187558 24.922596010717477 51 19.691872581125335 27.38612682345936 27.09288478713903 25.82911580827627 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 4.5 2 4.0 3 9.0 4 14.0 5 16.5 6 19.0 7 26.5 8 34.0 9 50.0 10 66.0 11 173.5 12 281.0 13 603.0 14 925.0 15 1291.0 16 1657.0 17 1712.0 18 1767.0 19 1653.5 20 1540.0 21 1502.5 22 1465.0 23 1653.5 24 1842.0 25 1913.0 26 1684.0 27 1384.0 28 1268.5 29 1153.0 30 1219.0 31 1285.0 32 1589.0 33 1893.0 34 1963.5 35 2034.0 36 1922.5 37 1811.0 38 1802.0 39 1793.0 40 1888.5 41 1984.0 42 2188.5 43 2393.0 44 2643.0 45 2893.0 46 3095.0 47 3297.0 48 3553.5 49 3810.0 50 4046.0 51 4282.0 52 4436.5 53 4591.0 54 4456.0 55 4321.0 56 3990.0 57 3659.0 58 3404.0 59 3149.0 60 2827.5 61 2506.0 62 2213.5 63 1921.0 64 1704.0 65 1487.0 66 1382.0 67 1277.0 68 1045.0 69 813.0 70 730.0 71 647.0 72 583.0 73 519.0 74 410.5 75 227.5 76 153.0 77 135.0 78 117.0 79 88.0 80 59.0 81 43.5 82 28.0 83 20.0 84 12.0 85 7.5 86 3.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14438821077701697 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.002977076510866329 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 67180.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.96508352531119 #Duplication Level Percentage of deduplicated Percentage of total 1 88.37255509961548 65.36483419288224 2 7.3554967185211915 10.880998583111444 3 1.9798873087860498 4.393275904951913 4 0.8038417461650145 2.378248875849091 5 0.39762909907478394 1.4705334762580313 6 0.2642210787395039 1.1725880494869136 7 0.222347017592383 1.1512141009477113 8 0.1164986164602397 0.6893463917651858 9 0.09064646288179252 0.6034205878493224 >10 0.33598081166756205 4.327296676679804 >50 0.03044975131233412 1.496952769716179 >100 0.028416536589132745 5.113455757678507 >500 0.002029752594552608 0.9578346328236885 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 638 0.949687406966359 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 436 0.6490026793688598 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 400 0.5954153021732659 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 379 0.5641559988091694 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 349 0.5194998511461745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 330 0.4912176242929443 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 281 0.4182792497767192 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 267 0.397439714200655 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 206 0.30663888061923195 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 141 0.2098838940160762 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.1979755879726109 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 133 0.1979755879726109 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 122 0.1816016671628461 RNA PCR Primer, Index 27 (95% over 21bp) ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 121 0.18011312890741293 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 108 0.1607621315867818 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 95 0.14141113426615065 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 94 0.1399225960107175 No Hit GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 75 0.11164036915748735 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 68 0.10122060136945518 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0014885382554331646 0.0 0.0 15 0.0014885382554331646 0.0 0.0 16 0.0014885382554331646 0.0 0.0014885382554331646 17 0.0014885382554331646 0.0 0.002977076510866329 18 0.0014885382554331646 0.0 0.004465614766299494 19 0.0014885382554331646 0.0 0.005954153021732658 20 0.0014885382554331646 0.0 0.011908306043465317 21 0.0014885382554331646 0.0 0.020839535576064303 22 0.0014885382554331646 0.0 0.03423637987496279 23 0.0014885382554331646 0.0 0.06549568323905924 24 0.0014885382554331646 0.0 0.10568621613575468 25 0.0014885382554331646 0.0 0.12652575171181898 26 0.0014885382554331646 0.0 0.1518309020541828 27 0.0014885382554331646 0.0 0.16969336111938077 28 0.0014885382554331646 0.0 0.2188151235486752 29 0.0014885382554331646 0.0 0.32003572491813037 30 0.0014885382554331646 0.0 0.5135456981244418 31 0.0014885382554331646 0.0 0.6996129800535874 32 0.0014885382554331646 0.0 0.8827031854718667 33 0.0014885382554331646 0.0 1.0657933908901458 34 0.0014885382554331646 0.0 1.2265555224769276 35 0.0014885382554331646 0.0 1.3813635010419767 36 0.0014885382554331646 0.0 1.554033938672224 37 0.0014885382554331646 0.0 1.7862459065197975 38 0.0014885382554331646 0.0 2.122655552247693 39 0.0014885382554331646 0.0 2.524560881214647 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGCTG 30 0.005113217 30.000002 10 CGGGCAG 120 1.6370905E-11 24.375002 4 ACGGGCA 125 2.7284841E-11 23.4 3 GGGCAGT 160 3.6379788E-11 19.687502 5 TACGGGC 185 1.4551915E-11 18.243242 2 AGTGGTA 190 4.2018655E-10 16.578949 9 GGCAGTG 195 6.0754246E-10 16.153847 6 CAGTGGT 195 6.0754246E-10 16.153847 8 GCAGTGG 205 1.2332748E-9 15.365853 7 GGGGTGG 370 0.0 14.594594 5 AACGCAG 985 0.0 14.390863 6 TACGGGA 130 1.8839765E-4 13.846153 2 GTACGGG 995 0.0 13.56784 1 ACGGGGT 420 0.0 13.392858 3 ACGGGTG 135 2.594214E-4 13.333334 3 CGCAGAG 1050 0.0 13.285715 8 CGGGTGG 120 0.001628315 13.125001 4 ACGCAGA 1075 0.0 12.976745 7 CGGGGTG 420 0.0 12.857142 4 CAGAGTA 1095 0.0 12.739726 10 >>END_MODULE