FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_1_1.341000000097d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_1_1.341000000097d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67180
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.949687406966359No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4360.6490026793688598No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4000.5954153021732659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.5641559988091694No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3490.5194998511461745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.4912176242929443No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2810.4182792497767192No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.397439714200655No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2060.30663888061923195No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.2098838940160762No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.1979755879726109No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1330.1979755879726109No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC1220.1816016671628461RNA PCR Primer, Index 27 (95% over 21bp)
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1210.18011312890741293No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1080.1607621315867818No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.14141113426615065No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA940.1399225960107175No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA750.11164036915748735No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT680.10122060136945518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTG300.00511321730.00000210
CGGGCAG1201.6370905E-1124.3750024
ACGGGCA1252.7284841E-1123.43
GGGCAGT1603.6379788E-1119.6875025
TACGGGC1851.4551915E-1118.2432422
AGTGGTA1904.2018655E-1016.5789499
GGCAGTG1956.0754246E-1016.1538476
CAGTGGT1956.0754246E-1016.1538478
GCAGTGG2051.2332748E-915.3658537
GGGGTGG3700.014.5945945
AACGCAG9850.014.3908636
TACGGGA1301.8839765E-413.8461532
GTACGGG9950.013.567841
ACGGGGT4200.013.3928583
ACGGGTG1352.594214E-413.3333343
CGCAGAG10500.013.2857158
CGGGTGG1200.00162831513.1250014
ACGCAGA10750.012.9767457
CGGGGTG4200.012.8571424
CAGAGTA10950.012.73972610
Produced by FastQC (version 0.11.2)