##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 6h_95.34100000004e4a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176171 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.29498612143883 38.0 38.0 38.0 32.0 38.0 2 36.250080887319704 38.0 38.0 38.0 32.0 38.0 3 36.203081097342924 38.0 38.0 38.0 32.0 38.0 4 36.301201673374166 38.0 38.0 38.0 32.0 38.0 5 36.30637278553224 38.0 38.0 38.0 32.0 38.0 6 38.22045058494304 40.0 38.0 40.0 38.0 40.0 7 38.121785083810614 40.0 38.0 40.0 38.0 40.0 8 38.05766556357176 40.0 38.0 40.0 32.0 40.0 9 38.08203960924329 40.0 38.0 40.0 32.0 40.0 10 37.99738322425371 40.0 38.0 40.0 32.0 40.0 11 37.989436399861496 40.0 38.0 40.0 32.0 40.0 12 37.96071998228994 40.0 38.0 40.0 32.0 40.0 13 37.981183055099876 40.0 38.0 40.0 32.0 40.0 14 37.96625437784879 40.0 38.0 40.0 32.0 40.0 15 37.981285228556345 40.0 38.0 40.0 32.0 40.0 16 37.96031128846405 40.0 38.0 40.0 32.0 40.0 17 37.97272536342531 40.0 38.0 40.0 32.0 40.0 18 37.979570985008884 40.0 38.0 40.0 32.0 40.0 19 37.9842312298846 40.0 38.0 40.0 32.0 40.0 20 37.97924743573006 40.0 38.0 40.0 32.0 40.0 21 38.005693332046704 40.0 38.0 40.0 32.0 40.0 22 37.99456777789761 40.0 38.0 40.0 32.0 40.0 23 37.97423526005983 40.0 38.0 40.0 32.0 40.0 24 37.987847034983055 40.0 38.0 40.0 32.0 40.0 25 37.99996594218118 40.0 38.0 40.0 32.0 40.0 26 37.927820129306184 40.0 38.0 40.0 32.0 40.0 27 37.91191512791549 40.0 38.0 40.0 32.0 40.0 28 37.87459911109093 40.0 38.0 40.0 32.0 40.0 29 37.80771523122421 40.0 38.0 40.0 32.0 40.0 30 37.75836545174859 40.0 38.0 40.0 32.0 40.0 31 37.69270765335952 40.0 38.0 40.0 32.0 40.0 32 37.61749096048725 40.0 38.0 40.0 32.0 40.0 33 37.50727985877358 40.0 38.0 40.0 32.0 40.0 34 37.408075108843114 40.0 38.0 40.0 32.0 40.0 35 37.33595767748381 40.0 38.0 40.0 32.0 40.0 36 37.186262211147124 40.0 38.0 40.0 32.0 40.0 37 37.13195134272951 40.0 38.0 40.0 32.0 40.0 38 37.036158050984554 40.0 38.0 40.0 32.0 40.0 39 36.91100691941352 40.0 38.0 40.0 32.0 40.0 40 36.79261626487901 40.0 38.0 40.0 32.0 40.0 41 36.721673828269125 40.0 38.0 40.0 32.0 40.0 42 36.54771216602052 40.0 38.0 40.0 27.0 40.0 43 36.38714657917591 40.0 38.0 40.0 27.0 40.0 44 36.19805189276328 40.0 38.0 40.0 27.0 40.0 45 35.985377843118336 40.0 38.0 40.0 27.0 40.0 46 35.6914418377599 40.0 38.0 40.0 27.0 40.0 47 35.41782699763298 38.0 38.0 40.0 13.0 40.0 48 35.16729768236543 38.0 38.0 40.0 13.0 40.0 49 34.93183895192739 38.0 38.0 40.0 13.0 40.0 50 34.75077623445402 38.0 38.0 40.0 13.0 40.0 51 34.148713465893934 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.2763390994251296 2201 2 -0.0882553670457824 2201 3 -0.3219860602352185 2201 4 0.04553258177457309 2201 5 0.05397984253340127 2201 6 0.06353804269470231 2201 7 -0.06480188510968787 2201 8 0.16948643844706623 2201 9 0.24076042942623133 2201 10 0.2590682733095875 2201 11 -0.017185787453946944 2201 12 -0.32795273724460117 2201 13 0.25234139034135694 2201 14 0.026243856586674497 2201 15 0.13960042946131068 2201 16 0.3817166484371626 2201 17 0.03495417682908197 2201 18 0.17429923118206148 2201 19 -0.09107335667093253 2201 20 0.13416988207392677 2201 21 -0.10389139757718624 2201 22 -0.08827799693128213 2201 23 0.11000651399316297 2201 24 -0.21093054270620115 2201 25 0.24488204563296279 2201 26 0.42179418666128043 2201 27 -0.20063981439592027 2201 28 -0.13620751531451702 2201 29 0.11938871438892562 2201 30 0.0816490946350612 2201 31 0.1395887419769437 2201 32 0.5077574985555486 2201 33 0.09740468815718373 2201 34 0.26572515709545996 2201 35 0.007483045536702093 2201 36 0.22682736340709653 2201 37 0.16650176132130667 2201 38 -0.023787978308924096 2201 39 0.599953461104775 2201 40 0.14052476755437482 2201 41 0.17217348957839107 2201 42 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48 -0.19233969050817024 2202 49 -0.19493166132674844 2202 50 -0.5871495845556822 2202 51 0.16784083412438378 1102 1 0.23508782586774402 1102 2 0.07120945594890316 1102 3 0.4160268710751964 1102 4 0.49476758593564085 1102 5 0.4353894834014653 1102 6 0.5173502186117318 1102 7 0.49956659322077 1102 8 0.27495343799895267 1102 9 0.05309638743148071 1102 10 0.30471195809288787 1102 11 0.36736065643569304 1102 12 -0.2544619235963026 1102 13 0.2173564341925811 1102 14 0.22587896317396172 1102 15 0.5083668336607872 1102 16 0.4240314810343051 1102 17 0.39523840959266465 1102 18 0.28194278793258576 1102 19 0.25896825398140066 1102 20 0.21643094090498494 1102 21 0.53633650076479 1102 22 0.33947950899023027 1102 23 0.29488584310141164 1102 24 0.5001955055621536 1102 25 0.7719929860337018 1102 26 0.433637218477422 1102 27 0.6437363459011181 1102 28 0.5006275144531003 1102 29 -0.01789443009606373 1102 30 0.470804718474767 1102 31 0.2532818474119338 1102 32 0.03121950482915281 1102 33 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3647.0 31 4583.0 32 5443.0 33 6479.0 34 7645.0 35 9169.0 36 11767.0 37 16010.0 38 25503.0 39 77114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.57421582210916 15.696050151043679 12.563313421309651 24.16642060553751 2 15.776597073215035 22.500368970175856 41.363827299251845 20.359206657357266 3 20.279160588292058 29.214796987018293 29.453201718784587 21.05284070590506 4 13.691810797463827 17.4762020990969 34.1554512377179 34.67653586572138 5 16.095157545793576 34.7894942981535 31.1441724233841 17.97117573266883 6 30.10086790674969 36.6025055202048 15.230088947670161 18.066537625375346 7 28.015961764422066 32.39239148327477 20.980751656061443 18.61089509624172 8 27.1906272882597 32.06657168319417 19.574163738640298 21.16863728990583 9 29.51393816235362 16.35002355665802 20.48180461029341 33.65423367069495 10 19.18931038593185 28.487662555131095 27.315505957280145 25.007521101656916 11 30.811280269745584 25.23415415006301 25.426586287932974 18.527979292258436 12 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20.278025327664597 25 19.662146437268333 27.71625295877301 32.18180063688121 20.43979996707744 26 20.104898081977172 26.65989294492283 32.673368488570766 20.561840484529238 27 19.218827162245773 29.1722247134886 32.23231973480311 19.37662838946251 28 18.35035278223998 28.241878629286322 30.495938605105266 22.91182998336843 29 20.026565098682532 28.61594700603391 31.085706501069982 20.271781394213576 30 21.285605621970188 29.121396864321152 30.101722239251615 19.491275274457045 31 21.895771721793032 27.93365536893132 30.972180438324127 19.198392470951518 32 20.532891338529044 27.63224367234108 30.853545702754708 20.981319286375168 33 18.998018970205084 28.367892558934216 30.836516793342835 21.797571677517865 34 21.323600365553922 27.73214660755743 32.218696607273614 18.72555641961503 35 19.36073474067809 29.14781660999824 29.813646968002676 21.67780168132099 36 19.531591465110605 30.128681792122425 30.28364486777052 20.056081874996455 37 18.623382963143765 31.77367444130986 30.616276231615874 18.9866663639305 38 19.62752098813085 28.61708226666137 31.669230463583673 20.086166281624106 39 18.356596715691005 29.65016943764865 30.662821917341677 21.330411929318675 40 19.64057648534662 28.43487293595427 32.051245664723474 19.873304913975627 41 18.90946864126332 28.682359752740233 29.754045785061106 22.65412582093534 42 20.976210613551608 28.368460189247948 30.216664490750468 20.438664706449984 43 18.40143951047562 28.528531937719602 31.169148157188186 21.900880394616596 44 18.22093307070971 30.34892235384938 29.979395019611626 21.45074955582928 45 17.768531710667474 28.32588791571825 32.34016949441168 21.56541087920259 46 20.10773623354582 27.938764041754887 28.07499531704991 23.878504407649388 47 17.098160310153204 27.886542052891794 29.420846790902022 25.594450846052986 48 18.00069250898275 27.59023902912511 28.329861327914358 26.079207133977782 49 20.070272632839682 27.422788086574972 29.082539123919375 23.424400156665968 50 18.611000737923597 27.508656411420784 30.319577680649374 23.560765170006242 51 17.84195857386941 27.326601161384804 28.532261635134443 26.29917862961134 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 32.0 1 42.5 2 53.0 3 102.5 4 152.0 5 201.0 6 250.0 7 263.5 8 277.0 9 389.0 10 501.0 11 768.5 12 1036.0 13 1924.5 14 2813.0 15 3702.0 16 4591.0 17 4757.0 18 4923.0 19 4567.0 20 4211.0 21 3979.5 22 3748.0 23 3960.5 24 4173.0 25 4175.0 26 3318.5 27 2460.0 28 2119.5 29 1779.0 30 2207.5 31 2636.0 32 4455.0 33 6274.0 34 7545.0 35 8816.0 36 9556.5 37 10297.0 38 8948.5 39 7600.0 40 6762.5 41 5925.0 42 5974.0 43 6023.0 44 6338.0 45 6653.0 46 7176.0 47 7699.0 48 8239.5 49 8780.0 50 9250.0 51 9720.0 52 10127.5 53 10535.0 54 11318.5 55 12102.0 56 10060.5 57 8019.0 58 7590.5 59 7162.0 60 6435.5 61 5709.0 62 5231.0 63 4753.0 64 4231.0 65 3709.0 66 3146.5 67 2584.0 68 2204.5 69 1825.0 70 1579.5 71 1334.0 72 1135.0 73 936.0 74 845.0 75 642.5 76 531.0 77 425.0 78 319.0 79 249.5 80 180.0 81 126.5 82 73.0 83 53.0 84 33.0 85 21.0 86 9.0 87 7.0 88 5.0 89 2.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03576070976494428 2 0.002838151568646372 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.002838151568646372 12 0.0 13 0.0 14 0.0 15 0.0 16 5.676303137292744E-4 17 5.676303137292744E-4 18 0.0022705212549170976 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.002838151568646372 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 5.676303137292744E-4 51 0.0011352606274585488 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 176171.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.80061604683783 #Duplication Level Percentage of deduplicated Percentage of total 1 84.86541380236119 54.99331095462819 2 8.706598392218401 11.283858789763203 3 2.8189279218166106 5.48004797776046 4 1.1392608040334824 2.952992077575418 5 0.7523005753970599 2.4374770369060026 6 0.44261963718831426 1.720921509853837 7 0.23747035024006877 1.077175749189036 8 0.20078962996329547 1.0409033373950523 9 0.10396572346665622 0.6063338635555058 >10 0.6480874383534871 7.353881896439741 >50 0.05009368771814373 2.180293504798777 >100 0.02652194296020176 3.3307080893595775 >500 0.006183406664665846 3.0057366207687104 >1k 0.0017666876184759348 2.5363585920064824 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC 3011 1.7091348746388453 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1420 0.8060350454955697 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 911 0.517111215807369 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 905 0.5137054339249933 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 903 0.5125701732975347 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 786 0.44615742659120966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 624 0.3542013157670672 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 570 0.3235492788256864 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 552 0.31333193317855945 No Hit GTACTAGTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACCTGT 398 0.22591686486425122 No Hit TTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCT 379 0.215131888903395 RNA PCR Primer, Index 36 (95% over 21bp) GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCTGT 367 0.20832032513864368 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 329 0.18675037321693128 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 319 0.18107407007963852 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 270 0.15326018470690408 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 260 0.14758388156961136 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 224 0.12714919027535745 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 222 0.1260139296478989 No Hit GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACCTGTCTCTTA 214 0.12147288713806473 No Hit GTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACCTGTCTCTTA 211 0.1197699961968769 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 198 0.11239080211839633 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 188 0.10671449898110358 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 187 0.10614686866737431 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 5.676303137292744E-4 9 0.0 0.0 0.0011352606274585488 10 0.0 0.0 0.0017028909411878233 11 0.0 0.0 0.0017028909411878233 12 0.0 0.0 0.0017028909411878233 13 0.0 0.0 0.0017028909411878233 14 0.0 0.0 0.0022705212549170976 15 0.0 0.0 0.0022705212549170976 16 0.0 0.0 0.0022705212549170976 17 0.0 0.0 0.002838151568646372 18 0.0 0.0 0.002838151568646372 19 0.0 0.0 0.002838151568646372 20 0.0 0.0 0.0034057818823756466 21 0.0 0.0 0.005676303137292744 22 0.0 0.0 0.008514454705939116 23 0.0 0.0 0.013623127529502586 24 0.0 0.0 0.0221375822354417 25 0.0 0.0 0.028949146000192993 26 0.0 0.0 0.03803123101986138 27 0.0 0.0 0.0488162069807176 28 0.0 0.0 0.08514454705939116 29 0.0 0.0 0.14928677251079916 30 0.0 0.0 0.23216079831527323 31 0.0 0.0 0.4308314081205193 32 0.0 0.0 0.6709390308280023 33 0.0 0.0 0.9627010120848494 34 0.0 0.0 1.383882704871971 35 0.0 0.0 1.7199198505997013 36 0.0 0.0 2.1218021127200277 37 0.0 0.0 2.7461954578222296 38 0.0 0.0 3.3569656753949286 39 0.0 0.0 4.274256262381436 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGAGAC 460 0.0 37.173676 22 TATGCCG 455 0.0 36.593178 43 TCTCGTA 455 0.0 36.593178 38 ATGCCGT 455 0.0 36.593178 44 TGCCGTC 465 0.0 36.290092 45 CTCGTAT 460 0.0 36.195423 39 CGAGACC 455 0.0 36.098675 23 CCCACGA 475 0.0 35.99977 19 AATCTCG 450 0.0 35.99977 36 CGTATGC 465 0.0 35.806225 41 CACGAGA 480 0.0 35.624775 21 CCACGAG 480 0.0 35.624775 20 GCAATCT 450 0.0 35.499775 34 GTATGCC 470 0.0 35.42531 42 TCGTATG 470 0.0 35.42531 40 GAGACCA 475 0.0 35.05241 24 GCAGCAA 460 0.0 34.72804 31 CAATCTC 460 0.0 34.72804 35 GACCAGC 470 0.0 34.46787 26 AGCCCAC 490 0.0 34.43856 17 >>END_MODULE