FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_95.34100000004e4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_95.34100000004e4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176171
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC30111.7091348746388453No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.8060350454955697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.517111215807369No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9050.5137054339249933No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9030.5125701732975347No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7860.44615742659120966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.3542013157670672No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5700.3235492788256864No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5520.31333193317855945No Hit
GTACTAGTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACCTGT3980.22591686486425122No Hit
TTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCT3790.215131888903395RNA PCR Primer, Index 36 (95% over 21bp)
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCTGT3670.20832032513864368No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3290.18675037321693128No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3190.18107407007963852No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2700.15326018470690408No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.14758388156961136No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2240.12714919027535745No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2220.1260139296478989No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACCTGTCTCTTA2140.12147288713806473No Hit
GTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACCTGTCTCTTA2110.1197699961968769No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.11239080211839633No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1880.10671449898110358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1870.10614686866737431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC4600.037.17367622
TATGCCG4550.036.59317843
TCTCGTA4550.036.59317838
ATGCCGT4550.036.59317844
TGCCGTC4650.036.29009245
CTCGTAT4600.036.19542339
CGAGACC4550.036.09867523
CCCACGA4750.035.9997719
AATCTCG4500.035.9997736
CGTATGC4650.035.80622541
CACGAGA4800.035.62477521
CCACGAG4800.035.62477520
GCAATCT4500.035.49977534
GTATGCC4700.035.4253142
TCGTATG4700.035.4253140
GAGACCA4750.035.0524124
GCAGCAA4600.034.7280431
CAATCTC4600.034.7280435
GACCAGC4700.034.4678726
AGCCCAC4900.034.4385617
Produced by FastQC (version 0.11.2)