FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_93.34100000004e20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_93.34100000004e20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574536
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGC207.03054E-444.99973729
GTATCAA4800.030.0076621
TATGCCG755.6292265E-826.99984643
GGCTAGC450.001223984225.0063841
CGCCTAA500.002263317822.4998739
GGTTACG500.002263317822.499879
AACGCAG6500.021.8075686
TCAACGC6450.021.6277834
GCCCACG955.522106E-721.31566618
CCACGAG955.522106E-721.31566620
CAACGCA6700.020.8207765
TCAATAC654.234843E-420.769113
ATCAACG6800.020.5145873
CTCGTAT1009.0392496E-720.24988439
CCCACGA1009.0392496E-720.24988419
CCAATCG1452.1827873E-1020.17229715
CTATATG908.22129E-620.0051081
CCTACAC807.554934E-519.6873843
TACGGGC1603.8198777E-1119.68738415
ACGGGCT1051.4430789E-619.28560316