FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_92.34100000004e13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_92.34100000004e13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291246
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC501.0768417E-940.49860845
TATGCCG451.9203071E-839.99862743
GCCCACG504.8590664E-835.99876418
CCCACGA504.8590664E-835.99876419
CCGAGCC504.8590664E-835.99876414
CGAGCCC504.8590664E-835.99876415
ATGCCGT651.3964382E-831.15277944
GTACTAC300.00513325630.0092751
TGCGGAT300.00514188629.998978
AAGCACG300.00514188629.9989718
CTCGTAT654.8538277E-727.69135939
CACGAGA654.8538277E-727.69135921
TCGTATG654.8538277E-727.69135940
CGAGACC507.142039E-526.99907323
TCCGAGC709.2703704E-725.71340413
CCACGAG709.2703704E-725.71340420
CTCCGAG751.6909034E-623.99917612
AGCCCAC751.6909034E-623.99917617
ACGAGAC751.6909034E-623.99917622
CATACGC1052.3501343E-923.5706238
Produced by FastQC (version 0.11.2)