FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_90.34100000004df8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_90.34100000004df8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186570
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.5386718121884547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.27013989387361315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.24012435010987834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGA401.5536592E-533.7497982
TATGCCG352.8114853E-432.14266643
TGCCGTC352.8114853E-432.14266645
CTCTCAC300.005129758730.0078661
TACTGTG300.005136490329.999827
GGTATCA4150.029.8270951
CGTATGC406.146856E-428.12483241
TCGTATG406.146856E-428.12483240
ATGCCGT406.146856E-428.12483244
ACGAGAC406.146856E-428.12483222
GTATCAA11050.028.1069131
CACGAGA450.001222748224.99985121
AATATTA450.001222748224.9998515
GTATGCC450.001222748224.99985142
TATCAAC14400.021.718622
CTCGTAT550.003924514620.45442439
CCCACGA550.003924514620.45442419
TCTCGTA550.003924514620.45442438
AACGCAG15400.020.3083216
GTAAGTA1008.972256E-720.249882
Produced by FastQC (version 0.11.2)