FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_9.34100000004833.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_9.34100000004833.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244078
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAA300.00513952529.99986513
GCACATA300.00513952529.99986512
ACGAAAG406.1522867E-428.1248723
TATGTAA608.157336E-626.2498822
ACCCGAC450.001223819824.99988611
ACCTTAC450.001223819824.99988628
CACCCGA450.001223819824.99988610
CCCGACT450.001223819824.99988612
TACTTAC500.002259600222.49989931
GCACCCG500.002259600222.4998999
CCGACTG500.002259600222.49989913
ATAGTCC602.459868E-422.4998996
TAGTCCA500.002259600222.4998997
TACTGTG955.49524E-721.3156937
TCTCCGA2700.020.83323945
CGAAAGG550.003927920.4544534
CTGCGTC550.003927920.45445311
CGCTCTC550.003927920.45445310
TGTCCCC550.003927920.45445310
CGAGCCC807.5321426E-519.6874144
Produced by FastQC (version 0.11.2)