##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 6h_88.34100000004dc1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11148 Sequences flagged as poor quality 0 Sequence length 51 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.26049515608181 38.0 38.0 38.0 32.0 38.0 2 36.283458916397564 38.0 38.0 38.0 32.0 38.0 3 36.276193039110154 38.0 38.0 38.0 32.0 38.0 4 36.438284894151415 38.0 38.0 38.0 32.0 38.0 5 36.41585934696806 38.0 38.0 38.0 32.0 38.0 6 38.38015787585217 40.0 38.0 40.0 38.0 40.0 7 38.16630785791173 40.0 38.0 40.0 38.0 40.0 8 38.163616792249734 40.0 38.0 40.0 38.0 40.0 9 38.319339791890926 40.0 38.0 40.0 38.0 40.0 10 38.227664155005385 40.0 38.0 40.0 38.0 40.0 11 38.22443487621098 40.0 38.0 40.0 38.0 40.0 12 38.03292070326516 40.0 38.0 40.0 32.0 40.0 13 38.1732149264442 40.0 38.0 40.0 38.0 40.0 14 38.00825260136347 40.0 38.0 40.0 32.0 40.0 15 38.11544671689989 40.0 38.0 40.0 38.0 40.0 16 38.03256189451022 40.0 38.0 40.0 32.0 40.0 17 37.95057409400789 40.0 38.0 40.0 32.0 40.0 18 37.941693577323285 40.0 38.0 40.0 32.0 40.0 19 37.963760315751706 40.0 38.0 40.0 32.0 40.0 20 38.001883745963404 40.0 38.0 40.0 32.0 40.0 21 38.07929673484033 40.0 38.0 40.0 38.0 40.0 22 38.19025834230355 40.0 38.0 40.0 38.0 40.0 23 38.164244707570866 40.0 38.0 40.0 38.0 40.0 24 37.98941514172946 40.0 38.0 40.0 32.0 40.0 25 37.93963042698242 40.0 38.0 40.0 32.0 40.0 26 37.84499461786868 40.0 38.0 40.0 32.0 40.0 27 37.956315034086835 40.0 38.0 40.0 32.0 40.0 28 37.888769285970575 40.0 38.0 40.0 32.0 40.0 29 37.95873699318263 40.0 38.0 40.0 32.0 40.0 30 37.86526731252243 40.0 38.0 40.0 32.0 40.0 31 37.898995335486184 40.0 38.0 40.0 32.0 40.0 32 37.860602798708285 40.0 38.0 40.0 32.0 40.0 33 37.82606745604593 40.0 38.0 40.0 32.0 40.0 34 37.80525654825978 40.0 38.0 40.0 32.0 40.0 35 37.885270900609974 40.0 38.0 40.0 32.0 40.0 36 37.96456763545031 40.0 38.0 40.0 32.0 40.0 37 37.805166846071046 40.0 38.0 40.0 32.0 40.0 38 37.7883925367779 40.0 38.0 40.0 32.0 40.0 39 37.73277717976319 40.0 38.0 40.0 32.0 40.0 40 37.6189451022605 40.0 38.0 40.0 32.0 40.0 41 37.75008970218873 40.0 38.0 40.0 32.0 40.0 42 37.751973448152135 40.0 38.0 40.0 32.0 40.0 43 37.881951919626836 40.0 38.0 40.0 32.0 40.0 44 37.833602439899536 40.0 38.0 40.0 32.0 40.0 45 37.87360961607463 40.0 38.0 40.0 32.0 40.0 46 37.646035163257984 40.0 38.0 40.0 32.0 40.0 47 37.64397201291712 40.0 38.0 40.0 32.0 40.0 48 37.69375672766415 40.0 38.0 40.0 32.0 40.0 49 37.59194474345174 40.0 38.0 40.0 32.0 40.0 50 37.530050233225694 40.0 38.0 40.0 32.0 40.0 51 36.89011481880158 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -2.0719580751409765 2201 2 -1.7791223636661542 2201 3 -3.0363752776901407 2201 4 -0.8546464019692692 2201 5 0.5420464908150677 2201 6 -1.0877541728520868 2201 7 -0.9768660653965142 2201 8 -1.1658236196130645 2201 9 -2.797673197051246 2201 10 -2.2197977275714393 2201 11 -0.2165505189768453 2201 12 -1.020322294504851 2201 13 0.27289713667723703 2201 14 -1.7240056562874457 2201 15 -0.15365282440328798 2201 16 0.18586499395065914 2201 17 -1.0205960527653772 2201 18 -0.47427480056456517 2201 19 -1.3879142692195785 2201 20 -2.6095255888722164 2201 21 -0.029982753633738923 2201 22 0.28217688501805327 2201 23 -0.3986227407060028 2201 24 -2.426124591915297 2201 25 -0.39475109098317773 2201 26 -0.9509960554574661 2201 27 0.44603811385734105 2201 28 0.19619994733177748 2201 29 -0.3824811166835076 2201 30 0.5982332468072826 2201 31 0.38517254127267364 2201 32 -0.4491091167632959 2201 33 0.40391980376497827 2201 34 1.2301268720679488 2201 35 -1.2664331104701745 2201 36 0.6926310422468589 2201 37 1.0870984931489645 2201 38 1.3035379478869302 2201 39 -0.7010565098061861 2201 40 -2.2297861409631565 2201 41 -1.3818561399266898 2201 42 -0.1420978088979652 2201 43 0.42967210377867104 2201 44 0.2161494810756608 2201 45 -1.5335721697246356 2201 46 -1.7397909547384671 2201 47 0.004013682532608698 2201 48 1.1838219411731998 2201 49 0.025339701691194705 2201 50 1.0064583713863442 2201 51 0.18759066597137064 1101 1 1.0351847820018776 1101 2 0.21194906490527643 1101 3 1.374339008024144 1101 4 1.2703535980307308 1101 5 0.631332205100783 1101 6 0.5283172557193438 1101 7 1.3088482203177705 1101 8 -0.13903790532734916 1101 9 1.2737553743773233 1101 10 -0.7644405847142934 1101 11 1.1584494810231547 1101 12 1.0511062769237185 1101 13 1.1121828509629523 1101 14 0.7134943437125543 1101 15 0.23920431845385792 1101 16 -0.5016350060493409 1101 17 1.068689661520338 1101 18 1.3292966280068654 1101 19 -0.0932714120767244 1101 20 0.8726172682706377 1101 21 0.7021601035091152 1101 22 -0.20889454355337733 1101 23 1.137091545008282 1101 24 0.9578039795132725 1101 25 1.4623917661596764 1101 26 -0.31706748402889673 1101 27 0.7853238281430563 1101 28 -0.2948714812396531 1101 29 1.6889474547450618 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1213 1 0.2851847820018776 1213 2 -0.06483664938043887 1213 3 -1.4292324205472866 1213 4 -2.5332178305407 1213 5 -0.6008106520420711 1213 6 -0.33775417285208675 1213 7 -0.7982946368250836 1213 8 -0.5586807624702033 1213 9 -0.26195891133696136 1213 10 -0.25551201328572404 1213 11 -0.4665505189768453 1213 12 -0.9488937230762815 1213 13 -1.5485314347513324 1213 14 -0.7597199420017304 1213 15 -0.3322242529747115 1213 16 -1.4569921489064868 1213 17 -0.9491674813368078 1213 18 -0.6528462291359887 1213 19 -1.3521999835052938 1213 20 -1.180954160300793 1213 21 -1.029982753633739 1213 22 -0.32496597212480793 1213 23 -1.0771941692774334 1213 24 -2.176124591915297 1213 25 -2.751893948126039 1213 26 -2.3081389126003273 1213 27 -3.4825333147140896 1213 28 -0.8038000526682225 1213 29 -1.346766830969223 1213 30 -0.4374810389070021 1213 31 -1.543398887298757 1213 32 -0.3776805453347194 1213 33 -0.9175087676635982 1213 34 -1.3413016993606206 1213 35 -1.0878616818987439 1213 36 -0.2716546720388564 1213 37 -0.7700443639938896 1213 38 -0.8036049092559239 1213 39 -0.37962793837760955 1213 40 -1.9083575695345871 1213 41 -1.3104275684981204 1213 42 -0.677812094612257 1213 43 0.0011006752072404424 1213 44 -1.5338505189243392 1213 45 0.5378564017039409 1213 46 -0.20407666902418242 1213 47 -0.8888434603245372 1213 48 -2.2804637731125084 1213 49 0.23962541597691 1213 50 0.149315514243483 1213 51 -3.098123619742914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 6.0 23 6.0 24 18.0 25 24.0 26 38.0 27 65.0 28 94.0 29 124.0 30 172.0 31 208.0 32 245.0 33 269.0 34 378.0 35 489.0 36 610.0 37 876.0 38 1713.0 39 5812.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.04808900053831 15.162390095101383 7.132603624618697 43.65691727974161 2 17.37531395766057 16.263006817366342 46.19662719770362 20.165052027269464 3 18.586293505561535 16.846071044133478 42.357373519913885 22.210261930391102 4 14.648367420165052 35.2081090778615 24.41693577323287 25.72658772874058 5 15.222461428058844 22.990670972371728 46.85145317545748 14.935414424111947 6 25.21528525296017 47.30893433799785 13.850017940437748 13.625762468604233 7 22.255113024757804 21.13383566559024 17.294581987800502 39.31646932185146 8 18.057050592034447 47.70362396842483 16.05669178327951 18.182633656261213 9 19.393613204162182 11.939361320416218 18.254395407247937 50.412630068173655 10 15.383925367778975 41.64872622891998 21.268388948690347 21.69895945461069 11 26.560818083961244 17.473986365267315 39.5496950125583 16.41550053821313 12 17.66236096160746 18.595263724434876 21.63616792249731 42.10620739146035 13 22.14747039827772 15.473627556512378 42.28561176892716 20.09329027628274 14 16.926803013993542 15.733763903839254 19.00789379260854 48.33153928955867 15 19.87800502332257 19.429494079655544 17.707212055974168 42.98528884104772 16 41.442411194833156 19.241119483315394 17.160028704700395 22.15644061715106 17 19.034804449228563 42.50089702188733 19.53713670613563 18.927161822748477 18 41.94474345174022 19.573017581628992 19.89594546106925 18.586293505561535 19 19.599928238249014 19.653749551489057 18.67599569429494 42.07032651596699 20 18.640114818801578 18.747757445281664 20.11123071402942 42.50089702188733 21 18.51453175457481 18.918191603875133 18.32615715823466 44.241119483315394 22 18.433799784714747 41.27197703623968 18.397918909221385 21.896304269824185 23 19.052744886975244 18.69393613204162 17.859705776820956 44.39361320416218 24 41.406530319339794 20.012558306422676 17.671331180480802 20.909580193756728 25 21.1248654467169 40.42877646214568 18.182633656261213 20.26372443487621 26 42.357373519913885 18.50556153570147 19.384642985288842 19.752421959095802 27 19.51022604951561 40.35701471115895 18.882310728381775 21.250448510943666 28 19.492285611768928 17.680301399354146 18.066020810907787 44.76139217796914 29 40.76067456045928 18.756727664155004 17.42016505202727 23.06243272335845 30 19.483315392895587 18.344097595981342 39.3702906350915 22.802296376031574 31 21.99497667743093 17.81485468245425 18.02116971654108 42.16899892357374 32 19.680660208109078 19.50125583064227 40.44671689989236 20.371367061356295 33 20.855758880516685 18.729817007534987 39.504843918191604 20.909580193756728 34 19.034804449228563 40.46465733763904 18.066020810907787 22.434517402224614 35 40.832436311446 18.68496591316828 18.49659131682813 21.98600645855759 36 19.72551130247578 19.214208826695373 39.7918909221385 21.268388948690347 37 20.057409400789382 39.477933261571586 18.057050592034447 22.407606745604593 38 19.590958019375673 17.590599210620738 41.13742375313958 21.681019016864013 39 18.83745963401507 17.599569429494082 19.93182633656261 43.63114459992824 40 19.483315392895587 17.016505202726947 42.13311804808038 21.367061356297093 41 19.061715105848585 18.415859346968066 19.74345174022246 42.77897380696089 42 41.18227484750628 17.743092931467526 19.967707212055974 21.10692500897022 43 19.19626838894869 17.294581987800502 41.07463222102619 22.434517402224614 44 20.147111589522783 39.047362755651235 19.797273053462504 21.008252601363473 45 17.985288841047723 16.101542877646214 43.95407247936849 21.95909580193757 46 39.89056332974524 17.75206315034087 19.851094366702547 22.506279153211338 47 17.28561176892716 16.71151776103337 21.824542518837458 44.17832795120201 48 17.859705776820956 16.989594546106925 20.999282382490133 44.15141729458199 49 41.02978112665949 16.73842841765339 19.7075708647291 22.52421959095802 50 18.84642985288841 16.092572658772873 42.491926803013996 22.56907068532472 51 18.406889128094726 17.868675995694293 20.218873340509507 43.50556153570147 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 1.0 16 2.0 17 1.5 18 1.0 19 1.5 20 2.0 21 2.0 22 2.0 23 2.5 24 3.0 25 2.0 26 4.5 27 8.0 28 7.5 29 7.0 30 12.0 31 17.0 32 24.0 33 31.0 34 70.0 35 109.0 36 175.5 37 242.0 38 359.0 39 476.0 40 626.0 41 776.0 42 850.5 43 925.0 44 881.0 45 837.0 46 727.0 47 617.0 48 661.0 49 705.0 50 1866.5 51 3028.0 52 1669.0 53 310.0 54 260.0 55 210.0 56 205.0 57 200.0 58 219.0 59 238.0 60 247.5 61 257.0 62 263.5 63 270.0 64 299.5 65 329.0 66 321.0 67 313.0 68 306.0 69 299.0 70 281.5 71 264.0 72 232.0 73 200.0 74 191.5 75 150.0 76 117.0 77 93.0 78 69.0 79 56.0 80 43.0 81 36.5 82 30.0 83 25.0 84 20.0 85 12.0 86 4.0 87 2.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01794043774668102 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 11148.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.10118406889129 #Duplication Level Percentage of deduplicated Percentage of total 1 88.18919675342849 56.53031933979189 2 7.780576546319619 9.974883387154646 3 2.225020990764064 4.278794402583423 4 0.6717044500419815 1.7222820236813776 5 0.517772180240694 1.6594904915679942 6 0.2518891687657431 0.9687836383207751 7 0.08396305625524769 0.3767491926803014 8 0.013993842709207949 0.07176175098672408 9 0.055975370836831795 0.32292787944025836 >10 0.18191995521970333 2.1528525296017222 >50 0.013993842709207949 0.8073196986006458 >100 0.0 0.0 >500 0.0 0.0 >1k 0.013993842709207949 21.13383566559024 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 2356 21.13383566559024 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG 90 0.8073196986006458 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 36 0.32292787944025836 No Hit CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTA 32 0.2870470039468963 No Hit GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTA 29 0.2601363473268748 No Hit GTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGC 21 0.1883745963401507 No Hit TTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTCT 21 0.1883745963401507 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT 17 0.15249372084678867 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 16 0.14352350197344815 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 13 0.11661284535342661 RNA PCR Primer, Index 27 (95% over 21bp) ACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGT 12 0.1076426264800861 No Hit CTTCTGGTTGTACAACGAGTACTAGTTCTGGTTGTACTACTGTAGTGACTT 12 0.1076426264800861 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.08073196986006459 2 0.0 0.0 0.8162899174739864 3 0.0 0.0 1.282741298887693 4 0.0 0.0 1.9375672766415502 5 0.0 0.0 2.1438823107283818 6 0.0 0.0 2.2425547183351275 7 0.0 0.0 2.4398995335486187 8 0.0 0.0 2.6462145676354503 9 0.0 0.0 3.3548618586293504 10 0.0 0.0 4.296734840330104 11 0.0 0.0 4.718335127377108 12 0.0 0.0 4.942590599210621 13 0.0 0.0 5.193756727664155 14 0.0 0.0 5.44492285611769 15 0.0 0.0 5.731969860064585 16 0.0 0.0 5.947255113024758 17 0.0 0.0 6.108719052744887 18 0.0 0.0 6.207391460351633 19 0.0 0.0 6.485468245425189 20 0.0 0.0 6.763545030498745 21 0.0 0.0 7.014711158952278 22 0.0 0.0 7.247936849659132 23 0.0 0.0 7.445281664872623 24 0.0 0.0 7.687477574452816 25 0.0 0.0 7.956584140653032 26 0.0 0.0 8.10010764262648 27 0.0 0.0 8.279512020093291 28 0.0 0.0 8.521707929673484 29 0.0 0.0 8.79978471474704 30 0.0 0.0 9.006099748833872 31 0.0 0.0 9.32902762827413 32 0.0 0.0 9.66092572658773 33 0.0 0.0 10.064585575888051 34 0.0 0.0 10.504126300681737 35 0.0 0.0 10.934696806602082 36 0.0 0.0 11.535701471115896 37 0.0 0.0 12.199497667743094 38 0.0 0.0 12.961966271977037 39 0.0 0.0 13.769285970577682 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 280 0.0 41.785717 45 ATGCCGT 280 0.0 41.785717 44 TATGCCG 295 0.0 39.66102 43 GTATGCC 300 0.0 39.0 42 CTCGTAT 305 0.0 38.360657 39 TCTCGTA 310 0.0 37.74194 38 CGTATGC 310 0.0 37.74194 41 TCGTATG 310 0.0 37.74194 40 ATCTCGT 330 0.0 35.454544 37 GTATCTC 320 0.0 35.15625 35 CTTAGTA 320 0.0 35.15625 31 AGTATCT 320 0.0 35.15625 34 TAGTATC 320 0.0 35.15625 33 TTAGTAT 320 0.0 35.15625 32 TATCTCG 335 0.0 34.25373 36 GTCTTAG 340 0.0 33.750004 29 TCTTAGT 340 0.0 33.750004 30 CGTCTTA 340 0.0 33.088238 28 AGACGTC 335 0.0 32.91045 25 GACGTCT 335 0.0 32.91045 26 >>END_MODULE