FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_88.34100000004dc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_88.34100000004dc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11148
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC235621.13383566559024No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG900.8073196986006458No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC360.32292787944025836No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTA320.2870470039468963No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTA290.2601363473268748No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGC210.1883745963401507No Hit
TTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTCT210.1883745963401507No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT170.15249372084678867No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC160.14352350197344815No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC130.11661284535342661RNA PCR Primer, Index 27 (95% over 21bp)
ACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGT120.1076426264800861No Hit
CTTCTGGTTGTACAACGAGTACTAGTTCTGGTTGTACTACTGTAGTGACTT120.1076426264800861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2800.041.78571745
ATGCCGT2800.041.78571744
TATGCCG2950.039.6610243
GTATGCC3000.039.042
CTCGTAT3050.038.36065739
TCTCGTA3100.037.7419438
CGTATGC3100.037.7419441
TCGTATG3100.037.7419440
ATCTCGT3300.035.45454437
GTATCTC3200.035.1562535
CTTAGTA3200.035.1562531
AGTATCT3200.035.1562534
TAGTATC3200.035.1562533
TTAGTAT3200.035.1562532
TATCTCG3350.034.2537336
GTCTTAG3400.033.75000429
TCTTAGT3400.033.75000430
CGTCTTA3400.033.08823828
AGACGTC3350.032.9104525
GACGTCT3350.032.9104526