FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_87.34100000004db4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_87.34100000004db4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC452.8790786948176583No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC342.1753039027511196No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC231.471529110684581No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC140.8957133717210494No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC140.8957133717210494No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC90.5758157389635317No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC80.5118362124120281RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC60.3838771593090211No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT50.3198976327575176No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC30.19193857965451055No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT20.12795905310300704Illumina PCR Primer Index 11 (95% over 21bp)
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA20.12795905310300704No Hit
GAAGTAACTATGTTCAGGCCCTAGGAAGTAACTATGCTGTCTCTTATACAC20.12795905310300704No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACCTGTCTCTTATACAC20.12795905310300704No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACCTGTCTC20.12795905310300704No Hit
CAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTA20.12795905310300704No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT20.12795905310300704No Hit
CTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACCTGTCTCTTATAC20.12795905310300704No Hit
GTCGTAAACCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTTA20.12795905310300704No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCCTGTCTC20.12795905310300704No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTT20.12795905310300704No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT20.12795905310300704No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC205.0708407E-445.00000445
AGCCCAC250.001523630836.017
GTATGCC250.001523630836.042
ATCTCGT250.001523630836.037
ATCTCCG250.001523630836.010
GCCCACG250.001523630836.018
CCACGAG250.001523630836.020
TATGCCG250.001523630836.043
GAGCCCA250.001523630836.016
CACGAGA250.001523630836.021
CCCACGA250.001523630836.019
TCTCGTA250.001523630836.038
ACGAGAC250.001523630836.022
ATGCCGT250.001523630836.044
CGTATGC250.001523630836.041
CGAGCCC250.001523630836.015
CCGAGCC250.001523630836.014
TCGTATG250.001523630836.040
TCTCCGA250.001523630836.011
TCCGAGC250.001523630836.013
Produced by FastQC (version 0.11.2)