FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_85.34100000004d9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_85.34100000004d9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC333.00820419325433No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC242.187784867821331No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC211.9143117593436645RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC151.367365542388332No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC100.9115770282588878No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC60.5469462169553327TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC30.27347310847766637No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC20.18231540565177756No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC20.18231540565177756No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCT20.18231540565177756No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC251.822089E-545.00000417
GTATGCC251.822089E-545.00000442
ATCTCGT251.822089E-545.00000437
ATCTCCG251.822089E-545.00000410
GCCCACG251.822089E-545.00000418
TGCCGTC251.822089E-545.00000445
CCACGAG251.822089E-545.00000420
TATGCCG251.822089E-545.00000443
CATCTCC251.822089E-545.0000049
GAGCCCA251.822089E-545.00000416
CACGAGA251.822089E-545.00000421
CCCACGA251.822089E-545.00000419
TCTCGTA251.822089E-545.00000438
ACGAGAC251.822089E-545.00000422
TATCTCG251.822089E-545.00000436
ATGCCGT251.822089E-545.00000444
CGTATGC251.822089E-545.00000441
CGAGCCC251.822089E-545.00000415
CCGAGCC251.822089E-545.00000414
TCGTATG251.822089E-545.00000440