FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_83.34100000004d71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_83.34100000004d71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332845
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTCA207.0280203E-444.99744419
CGCTCAG250.002105548735.9979620
CGACCTC453.4848235E-529.99829714
GACCTCG453.4848235E-529.99829715
CGGGTGT300.005143494329.99829517
GGGTTTA507.138835E-527.0025257
ACCTCGA507.146143E-526.99846816
ACGACCT507.146143E-526.99846813
CAATTAG608.1437465E-626.2603471
CGTAACG450.001225129424.9985814
GTTTACG551.3644857E-424.5477499
CGTCTTA551.3644857E-424.54774928
AGACGTC551.3644857E-424.54774925
TTACGAC551.3644857E-424.54774911
TACGACC551.3658786E-424.5440612
GAACGTC500.002260026822.5021045
AACTAGC500.002260026822.5021046
CGGGGTA500.002262001422.49872417
TAGTATC602.463349E-422.49872233
TGCCGTC602.463349E-422.49872245
Produced by FastQC (version 0.11.2)