FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_82.34100000004d64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_82.34100000004d64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333117
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC5790.17381280451012707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG855.456968E-1231.76311743
TATTCCC751.6916601E-930.0030045
GAGTGCA300.005139556730.0030027
ATATGTA602.408142E-730.0030021
TCTACGG300.005139556730.0030026
ACGCCTA602.411307E-729.99849921
TGCCGTC901.2732926E-1129.99849945
CTCGTAT1053.6379788E-1227.85574739
CGCCTAA654.8580296E-727.69092422
GGTATCA3200.026.018231
GTATCAA8000.025.877591
CGTCTTA801.04961146E-725.31123528
CGTATGC1251.8189894E-1225.19873841
ACTTACC551.365819E-424.54422832
TACTTAC1750.024.4273531
ATCCTAT751.6901449E-624.0024031
AAAACGC751.6901449E-624.00240318
GCCCACG1152.6375346E-1023.48061218
TAATCAA903.2740172E-722.49887526
CCCACGA1204.4929038E-1022.49887519
Produced by FastQC (version 0.11.2)