FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_81.34100000004d4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_81.34100000004d4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231366
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5140.22215883059740843No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3870.16726744638365187No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3750.1620808588988875No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.14047007771236913No Hit
TAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGCTGTCTCTTATACAC2920.12620696212926705No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC2740.11842708090212045No Hit
TGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGCTGTCTCTTATAC2680.11583378715973826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.10848612155632202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.10243510282409687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGTA504.8412403E-836.007611
TATGCCG501.9726613E-631.4998543
TGCCGTC501.9726613E-631.4998545
GTATTGG1350.030.0063421
GACGTCT453.479043E-529.99985726
CGAGACG453.479043E-529.99985723
TCGATTT300.005138984429.99985517
CGGAGTT300.005138984429.99985536
ATGCCGT554.146221E-628.63622544
CCACGAG554.146221E-628.63622520
ATCTAGA406.143544E-428.1309451
GAGACGT507.134361E-526.99987224
ACGTCTT507.134361E-526.99987227
CTCGTAT608.1549515E-626.24987239
CACGAGA608.1549515E-626.24987221
TCTCGTA608.1549515E-626.24987238
ACGAGAC608.1549515E-626.24987222
ATAATGA801.0462463E-725.3123784
AGGAGCC450.001223628924.9998828
TAGACTC450.001223628924.999885
Produced by FastQC (version 0.11.2)