FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_8.34100000004819.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_8.34100000004819.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC3515.555555555555555No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC198.444444444444445No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC94.0No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC62.666666666666667No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC41.7777777777777777No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC41.7777777777777777RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC31.3333333333333335No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC31.3333333333333335No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.8888888888888888No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT20.8888888888888888Illumina Paired End PCR Primer 2 (95% over 21bp)
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.8888888888888888No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20.8888888888888888TruSeq Adapter, Index 12 (95% over 21bp)
GTCTAGGGTAGTGTTGCTCAGTGTTGACATTTTGAGCCAAATAATTTTTTG10.4444444444444444No Hit
TTATACACATCTCCGAGCCCACGAGTCGATAACCTATCTCGTATGCCGTCT10.4444444444444444No Hit
GTATTTGTACAAGAATGCTTACTAGTAACTGATGTCAGACATTGTTGTAAC10.4444444444444444No Hit
GACCTGCAATTTCAAACTGGACAGAAAGCAAACTCAAACCAACAGAAACCT10.4444444444444444No Hit
GTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT10.4444444444444444No Hit
TCAATGGCTATATCCAGAAGATTAAGTCAGGAGAGGAAGACTTTGAATCTC10.4444444444444444No Hit
GTCCCAGCATAGTCGTCAGTTCCCTCTGGGCTGGAGTCTCCGTCTTAATGT10.4444444444444444No Hit
CAAATGCCTACTTTACAGTTTGCCTCTGAGATTTTAGATTAGTGATACATT10.4444444444444444No Hit
CCTCTTTTCCTTTTTTTTTTTTTTTTTTTTGGGGGAATCTACTTTTTTTTA10.4444444444444444No Hit
TGATAGAAGACATCAAGAAGGACTTTCATAAGTCACTTAACTGTCTCTTAT10.4444444444444444No Hit
CTGCTGCTGAGAAGGCTGTGACCAAGGAGGAATTCCAGGGTGAATGGACCG10.4444444444444444No Hit
GAAGTAATCTGCTTAACAATCTCAGAAGGACTATGTAAATCAATGAGTCGC10.4444444444444444No Hit
CTAATATACGGCATCACAGTTTTCTCCACAAATGAGCCAAAGCCAATGCCT10.4444444444444444No Hit
CCATGACAGTAGAAGGACCTCCTCCCAAAGATACTGGCATTGCCCGAGTGC10.4444444444444444No Hit
CTGCAATGGAGATGTCACTTCCACCATCCTTCAGATCGTCCTGGAGATATT10.4444444444444444No Hit
CTTATACACACCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC10.4444444444444444No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTT10.4444444444444444No Hit
CTTATACACATCTCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT10.4444444444444444TruSeq Adapter, Index 12 (95% over 22bp)
CAGACGTACATGGCGCGGGCTTTGCGGGCTAATCCGAACCTGGCAAGGGAC10.4444444444444444No Hit
GATGCATACAGTAACTTACTTCAAAACAGCCTTCTAAATGGGCCTTTCTAA10.4444444444444444No Hit
GTGCTGGGATTAAGGTGTACACCACTAAGCTTCTGCCTCCTCAAGTGTTGA10.4444444444444444No Hit
GTGATCCGCCTGAACTACTGGCTTGGCCGCTGGCGCGGCCCGCCCAGTGTG10.4444444444444444No Hit
GTACACACACACACTGGCACACGCACACACATACCTGTCTCTTATACACAT10.4444444444444444No Hit
GTCTACAGAGCTGTTCTTGTCATGTACATCTGTGACTTCTGCGTTGCCAAG10.4444444444444444No Hit
ATCCTACTCTCTACAAACACTTATTACAACCCAAGAACACCTGTCTCTTAT10.4444444444444444No Hit
TGCTACTGTGTCAGGGTTGTAGCCAATTTTCTTAATGTAGGTGCTGACCTG10.4444444444444444No Hit
GTTTTGCTCAGTTTGAGTTATCGTCTGGGAAAGAAGGAAGTGAGGCTAAAT10.4444444444444444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGCGA10.4444444444444444No Hit
AAAAAATACACAAACAGCCTGCAGAAATCATCAAAAGACATGGTATATTCT10.4444444444444444No Hit
TTCTGGTCCCTTCTAAGTCCAACTCTACAACAGATGAGCTTTTCAATGCTC10.4444444444444444No Hit
CTTAGGCTACTTGGCACGAAAGCAATATAGAAAGGTCCTCTGTGGTGTCTG10.4444444444444444No Hit
GCAGAGAATGTACTTGTTGCTGAGTGAGATCTAAGGTAAGACCCGATTATT10.4444444444444444No Hit
TTTCTTGGCTCTTTGTGCCAGTAATTACTACAACGGCTGTGTGTTTCATAG10.4444444444444444No Hit
GGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC10.4444444444444444No Hit
ATTCTGCACATCACTGGGCCCTTCCTCCACCCCATCCTCCTGGTTCACCCA10.4444444444444444No Hit
GCTTCCGCTGAGCTCTTCCGAGCTGCTCGCTCTCCACACGCGCCGCCGCCG10.4444444444444444No Hit
GTCTCTGATTCTGTAAACTGGGCTTCTCAGAGGTTTCTGTGTGGAACAGGA10.4444444444444444No Hit
TCTTTAATCAGTGAAATTGACCTTTCAGTGAAGCTGTCTCTTATACACATC10.4444444444444444No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTCTGTCTCT10.4444444444444444No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC10.4444444444444444No Hit
CACTTGTTCAGCTTGGTTCAGCTGTCTTGCTGCTCTGACACTTCATTTTGA10.4444444444444444No Hit
GTTGGCTACTAGGATTCAGTACAAAATTTGTGTGATTGGGCGGAATATTAG10.4444444444444444No Hit
CCTTTTGGAGAACTGTTTCTCAGAAACAACTGTGATTTTATTCTTCAGGCG10.4444444444444444No Hit
CTGTGTACATTTTCATGATGATGATTGGATGGATGGAGACAAAAGAGAGTG10.4444444444444444No Hit
TTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGGTAGAAAAT10.4444444444444444No Hit
GGATTGGACGTCAGGAAGCCCGGGCGGCCAGGGCAGCGAACCGCCTGCGCC10.4444444444444444No Hit
GACATAGACTCCTTTGAATCAGAACTTCCCGATGATGACTTCCTCTCTCTC10.4444444444444444No Hit
CTACTAGCCATAGCTTAAAACTCAAAGGACTTGGCGGTACTTTATATCCAT10.4444444444444444No Hit
ATCTTGATGAAGAGGCACTGGTCAGAGCAGCTTTGAGCCTCTATCCGGGCA10.4444444444444444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGAAAGCGGGGGT10.4444444444444444No Hit
GTCTCATTATTTTCGTCATTCTCTACCTTCATACGCTTCACCACACTACAA10.4444444444444444No Hit
TGCCAACTCTGGAAGAGCTGAAAGTGGAGGAGGTGAAAGTCAGCTCAGCTG10.4444444444444444No Hit
CTTATACACATCTCCGGCCCACGAGACAACATAATATCTCGTATGCCGTCT10.4444444444444444RNA PCR Primer, Index 44 (100% over 21bp)
ATATAAAGTAAGGTGTAGCGTATATTTGTGAATGTGCAATGCATTTAAGTG10.4444444444444444No Hit
GAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTACT10.4444444444444444No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGTGAGTATGTGTGCCT10.4444444444444444No Hit
CTTGTAGGGAGGAAAATATGTTCAATTACCAATTGTGGAACATTATGGGTG10.4444444444444444No Hit
TCTTAGAGGCTCAGATCTGTTGTATCAGTTGAGGGGGACCTTGGGCCTGCC10.4444444444444444No Hit
CTTATACACATCTCCGAGCCAACGAGACCCTGGATAATCTCGTATGCCGTC10.4444444444444444No Hit
CAGGAAATTCTTAGGAAAAGTCTGGGTTTGTCTCAGCGTTTTTTAAGTTTT10.4444444444444444No Hit
CATTTGTTCTTCCTCTTCATCTGCTTCTTCAATAACCTGAATCTCATCTGC10.4444444444444444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4444444444444444No Hit
GGCATGGTGAATAGATTTGGCCAGGCCCAGCTTAAAGACCCTGTCTCTTAT10.4444444444444444No Hit
GTACTAGTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCCTGTCTCTT10.4444444444444444No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGATCGTATGCCGTCTTC10.4444444444444444No Hit
GTTGGTGAAGATTCCACATATAAATTTTTTGAGGTTATCCTCATTGATCCT10.4444444444444444No Hit
GAACAGCTGTTAGTGAAATAAATGATCCTATAGAAGAGACAGTGTTTCATG10.4444444444444444No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTATATCTCGTATGCCGTC10.4444444444444444No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGCA10.4444444444444444No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC10.4444444444444444No Hit
AGCAAGCACACCCGTGATTGTGAAAATAGTGTAGCAAAAAAGAAAAGAAAC10.4444444444444444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAA10.4444444444444444No Hit
GTGTTTGTAGAGAGTAGGATCCATTTATTAATAGAACTGATAAAAGGATAA10.4444444444444444No Hit
GTCTGAACACGCAGCATACAAACTGGGAAAACAGTTTTGTAAGCAATACAT10.4444444444444444No Hit
GCCTTTAGGAACGAGGTGACTAGGTTAGGGAAATGCATTAGTGTTTGGGCT10.4444444444444444No Hit
GGCCAGAAGTGGCTGTTCCTCAGAATGAAGTCCCTCGGCCTTCTTAGCTAT10.4444444444444444No Hit
CTACAAGAAAAAAGGAGGGGAAAATGGCATTGTATCATCATCACTTCATCA10.4444444444444444No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTACTTCGCATGCCTTC10.4444444444444444No Hit
TAATGAAGGTTGAACTACTGCCTGCCCTGACTGACAACTACATGTACCTGA10.4444444444444444No Hit
GTAGTCTCTCACTAAGTTGTCGATGAAGTTCATAACATTTTCTCTGAGCAT10.4444444444444444No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCCTGTCTCT10.4444444444444444No Hit
GAATGTGACAAATCCTTTACGCAAAAAGGCAGTCTTAGTATTCATCAGATG10.4444444444444444No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA10.4444444444444444No Hit
TAACAAGATCTGACTCCCTCTTCTGGTGTGTCTGAAGACAGCTACAGTGTC10.4444444444444444No Hit
CTCAAGTAGTGTATAGGGACTGAGTATGATGGTATATGCTCTGTCTCTTAT10.4444444444444444No Hit
CTGTGAGGTCATCGCCCACCACCTGGATGCCCGCACTAGCCGTGAACTTCT10.4444444444444444No Hit
ACTTTGAGCAGCAATCCCAAACTACTCGACTCTTGGAGCGTGTATCCTGTC10.4444444444444444No Hit
CCTCTGTATAGTATCCCCGTGGCTCCCCCAGCGGCTGTCTCTTATCCACAA10.4444444444444444No Hit
GTTAGAAATTCTGTTTAGAACAGGAACACTTCAGCAGAGTAATGGCCATTT10.4444444444444444No Hit
ACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.4444444444444444No Hit
CTTATACACATCTCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT10.4444444444444444No Hit
AAATTAGCCAGGTGTGATGGTGCAACCTGTGGTCCCAGCTATTCCAGAGCC10.4444444444444444No Hit
CTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTT10.4444444444444444No Hit
TCCCTTCACCACTCAATGGTGACTCCAAGAGCAGCTCAAAAAGCCAGTGAA10.4444444444444444No Hit
TATCAACGCAGAGTACGGGATGACAAACATACGCAAAACACACCCCGTCTC10.4444444444444444No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCAAGTATTTTAGTGATT10.4444444444444444No Hit
CTACATTGAAAAAAAAATGTATGTGGTTTCAACTGTTTGGATTTACTCTGG10.4444444444444444No Hit
GTACTTGGCGTGCTGGCTGATTTCAATTTTCTTCACCATTTTCCGGAGGGA10.4444444444444444No Hit
AGCGTAGTCTTTTTCATGGTATTCACATATCCCTTTCCTCAGAAGAGCAGT10.4444444444444444No Hit
GGCAAGGATCTTACATGTTTAACAGGGAGTTGGCTGGATAATTTCAAATAC10.4444444444444444No Hit
TGACTATAGTCTGCAGCACAACTTTTCTTTTAACAAAGCTAGAACAGTTTT10.4444444444444444No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC10.4444444444444444No Hit
ATACACACTTTAGGACGTGAAAGATGGCGAGGAAAATTGAAAAAGGTGGAA10.4444444444444444No Hit
CTTATCACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT10.4444444444444444Illumina Paired End PCR Primer 2 (95% over 21bp)
ATACAGTCCCAATCTCGTTCCACATTGCGTCAAGGTCCCGTACCTGTCTCT10.4444444444444444No Hit
CTCTGCATCTGTAGACTTAAAGTGATCGTCGTCCACAGTCTGTCTCTTATA10.4444444444444444No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGCT10.4444444444444444No Hit
GCCGTGGCAAAGGCTGATAAGCTGGCAGACTGTCTCTTATACACATCTCCG10.4444444444444444No Hit
ACTTCCACTTGGTCTTAATAAAAAGCTCTCCTACCACATGTTCCAAAAACA10.4444444444444444No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTT10.4444444444444444No Hit
AAGAAGAACAGATAGCCACTGTTAAAGTGAGATCACTAAGTCTTCATTACT10.4444444444444444No Hit
CTTATACACATCTCCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT10.4444444444444444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4444444444444444No Hit
GTACGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTG10.4444444444444444No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC10.4444444444444444No Hit
GTCCTGGGCTGGCAGCATGCACCAGTGCTTTCACTCGCATCTCCATCTTGA10.4444444444444444No Hit
CCTAAAGTGTGTATTTCTCATTTCCGTGATTTTCAGTTTTCTCGCCATATT10.4444444444444444No Hit
GTAGTGAGCTTTCTCTACAGAGGTTTGAAAATGATCTGGCACTAGAATCCC10.4444444444444444No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG10.4444444444444444No Hit
GCCTTCAGGAATGCAGGGCTCTCCACTTGTCCAGAGGACCACGACTGTCTC10.4444444444444444No Hit
CATTTAGCTGGCCCAGTGCTCAGGCCAGAGCATGCCTCTGGGCCTGCACCT10.4444444444444444No Hit
CGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGAC10.4444444444444444No Hit
GAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTG10.4444444444444444No Hit
GTTCAAGGAGTATTGAGTTGTTTTTCTGGATTAGGGTAGCAAGAGATCTGT10.4444444444444444No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGCTGT10.4444444444444444No Hit
GGGCGCAGGGATCGTTCGTGCCGCTAAAAGTCATGAGCTGGTCCGGGACCA10.4444444444444444No Hit
GTACGGGGTTGGAACAGTGCACACAAGACGAACAGATGGGACCTTTGTCAC10.4444444444444444No Hit
GTTACAAAGCTGCCTAGTGGCTTGAGAGGTTCATTCAGGCTGTCTCTTATA10.4444444444444444No Hit
CATCTTTATTACTGGACATGAGCATGTTTTAGCACACTGGTTTCATGTGTA10.4444444444444444No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTAACCTATCTCGTATGCCGTC10.4444444444444444No Hit
TGTTGCAGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTTTGG10.4444444444444444No Hit
CTTATACACATCTCCGAGCCCACGCGACGACGCTATATCTCGTAAGCCGTC10.4444444444444444No Hit
TGCTGGAAGAGGAAGAAGAACATGAATGGTTTATTCCCTGTCTCTTATACA10.4444444444444444No Hit
GTGCACTTACACTCACCACCTGATTACCAACCAGGCTGAGGGAACCTTTGG10.4444444444444444No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers