FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_79.34100000004d21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_79.34100000004d21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC863.3398058252427183No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC501.9417475728155338No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC230.8932038834951457No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC220.854368932038835No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC200.7766990291262136TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC200.7766990291262136No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC130.5048543689320388RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC100.3883495145631068No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT60.23300970873786409No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT50.1941747572815534Illumina PCR Primer Index 8 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT40.15533980582524273No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC30.11650485436893204No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT30.11650485436893204TruSeq Adapter, Index 12 (95% over 22bp)
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAGTACTCTGCGTTGA30.11650485436893204No Hit
CTTCTGGAGTGTCTGAAGACAGCTACAGTGTACCTGTCTCTTATACACATC30.11650485436893204No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC30.11650485436893204RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC205.790163E-445.013
AGCCCAC250.001737761936.017
GCCCACG250.001737761936.018
CCACGAG250.001737761936.020
GAGCCCA250.001737761936.016
CCCACGA250.001737761936.019
CGAGCCC250.001737761936.015
CCGAGCC250.001737761936.014
TCTCCGA250.001737761936.011
CTCCGAG250.001737761936.012
ATCTCCG300.00425252629.99999810
ACATCTC300.00425252629.9999988
CACATCT350.00903924425.7142857
CATCTCC350.00903924425.7142859
Produced by FastQC (version 0.11.2)