FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_78.34100000004d14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_78.34100000004d14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616944
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8050.033.833221
GTAATAA300.005135515230.0118391
GTGGTAT2000.025.885211
GTATCAA17950.025.4557251
TAGGTAC450.001225987324.9997315
ACGGGTA1152.6375346E-1023.47800816
CGTTACA500.002263572522.49975624
ACAATAC500.002263572522.4997563
TGGTATC2550.022.0603732
ACCGTGC855.0264443E-621.176248
CTCGTAT754.5985784E-520.99977339
CCGTGCA754.5985784E-520.9997739
TCGTATG754.5985784E-520.99977340
TATCAAC22950.019.9033582
TATGCCG807.556376E-519.68728643
ATGCCGT807.556376E-519.68728644
ATCAACG24050.018.991483
GGCCTAA600.00649235218.7573991
TCAACGC24450.018.680784
CCCTTAA851.1996586E-418.5367221
Produced by FastQC (version 0.11.2)