FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_77.34100000004d07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_77.34100000004d07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC542.530459231490159TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC391.8275538894095593No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC351.640112464854733RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC331.5463917525773196No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC281.3120899718837862No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC271.2652296157450795No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC120.5623242736644799No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT80.37488284910965325TruSeq Adapter, Index 12 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT70.32802249297094654No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC60.28116213683223995No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT40.18744142455482662RNA PCR Primer, Index 38 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT30.14058106841611998Illumina PCR Primer Index 8 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC30.14058106841611998No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC205.544108E-445.017
GTATGCC205.544108E-445.042
ATCTCGT205.544108E-445.037
ATCTCCG205.544108E-445.010
GCCCACG205.544108E-445.018
TGCCGTC205.544108E-445.045
CCACGAG205.544108E-445.020
TATGCCG205.544108E-445.043
GAGCCCA205.544108E-445.016
CACGAGA205.544108E-445.021
CCCACGA205.544108E-445.019
TCTCGTA205.544108E-445.038
ACGAGAC205.544108E-445.022
ATGCCGT205.544108E-445.044
CGAGCCC205.544108E-445.015
CCGAGCC205.544108E-445.014
TCGTATG205.544108E-445.040
TCTCCGA205.544108E-445.011
TCCGAGC205.544108E-445.013
CTCGTAT205.544108E-445.039
Produced by FastQC (version 0.11.2)