FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_76.34100000004cf9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_76.34100000004cf9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671622
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA250.002106811635.9988675
CGATCTA300.0051446730.00129310
TATGCCG651.5228967E-524.2300143
CATTTAC602.4603435E-422.5076711
TCGTATG802.9725106E-622.49929240
GGTATCA9550.022.3898281
GTAAGTA1601.8189894E-1221.1009431
GTTTAGG550.00392709720.4615211
TCTCCGA7950.020.09370845
CTCGTAT908.245106E-619.9993739
GTATCAA16300.019.7459931
CAATACA706.9808104E-419.2851094
CAGTCCG951.3148583E-518.9467729
CTAACCC1555.329639E-1018.8703753
TCTAACC1555.329639E-1018.8703752
GATCTAA600.00650612718.75080911
CGTATGC851.2037851E-418.5288341
TAGAAGT1253.828609E-717.9994354
CTGCGAC750.00110980417.99943528
TAGATCG750.00110980417.9994355
Produced by FastQC (version 0.11.2)