FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_75.34100000004cec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_75.34100000004cec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691449
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTT250.002106578335.9998311
GGTATCA4550.026.7089611
GTATCAA10850.026.3419931
TAGCCTA651.5226675E-524.230655
CGACCAT654.2360162E-420.76912925
TATCAAC14750.019.2202452
CATCTCC23950.019.07088945
TATGCCG600.006508114718.74990843
GCGCAAT600.006508114718.74990821
ATCTCCG9550.018.61247645
CAACGCA15500.018.2902345
GCTTAAC750.001108036318.0038191
ACCTTAC750.001108036318.0038191
TCAACGC15750.017.9999124
AACGCAG15900.017.8301036
ATCAACG16200.017.6388033
ACGCAGA16350.017.476987
CGCAGAG16650.017.1620798
CCTAGAT800.001709969516.874923
TACTCAC800.001709969516.8749231
Produced by FastQC (version 0.11.2)