##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 6h_74.34100000004cdf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5206 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.921052631578945 38.0 38.0 38.0 32.0 38.0 2 35.90760660776028 38.0 32.0 38.0 32.0 38.0 3 35.890703034959664 38.0 32.0 38.0 32.0 38.0 4 36.08029197080292 38.0 32.0 38.0 32.0 38.0 5 36.11851709565885 38.0 38.0 38.0 32.0 38.0 6 38.03169419900115 40.0 38.0 40.0 38.0 40.0 7 37.76699961582789 40.0 38.0 40.0 32.0 40.0 8 37.78985785631963 40.0 38.0 40.0 32.0 40.0 9 37.94602381867077 40.0 38.0 40.0 32.0 40.0 10 37.80138301959278 40.0 38.0 40.0 32.0 40.0 11 37.82039953899347 40.0 38.0 40.0 32.0 40.0 12 37.55186323472916 40.0 38.0 40.0 32.0 40.0 13 37.72243565117172 40.0 38.0 40.0 32.0 40.0 14 37.583173261621205 40.0 38.0 40.0 32.0 40.0 15 37.742220514790624 40.0 38.0 40.0 32.0 40.0 16 37.40165194006915 40.0 38.0 40.0 32.0 40.0 17 37.389358432577794 40.0 38.0 40.0 32.0 40.0 18 37.17364579331541 40.0 38.0 40.0 32.0 40.0 19 37.40971955436035 40.0 38.0 40.0 32.0 40.0 20 37.371110257395316 40.0 38.0 40.0 32.0 40.0 21 37.524587014982714 40.0 38.0 40.0 32.0 40.0 22 37.64636957356896 40.0 38.0 40.0 32.0 40.0 23 37.62754514022282 40.0 38.0 40.0 32.0 40.0 24 37.53860929696504 40.0 38.0 40.0 32.0 40.0 25 37.318478678447946 40.0 38.0 40.0 32.0 40.0 26 37.21494429504418 40.0 38.0 40.0 32.0 40.0 27 37.20956588551671 40.0 38.0 40.0 32.0 40.0 28 37.26680752977334 40.0 38.0 40.0 32.0 40.0 29 37.380906646177486 40.0 38.0 40.0 32.0 40.0 30 37.31751824817518 40.0 38.0 40.0 32.0 40.0 31 37.34056857472147 40.0 38.0 40.0 32.0 40.0 32 37.152132155205535 40.0 38.0 40.0 32.0 40.0 33 37.239723396081445 38.0 38.0 40.0 32.0 40.0 34 37.43507491356127 40.0 38.0 40.0 32.0 40.0 35 37.36573184786784 40.0 38.0 40.0 32.0 40.0 36 37.336726853630424 40.0 38.0 40.0 32.0 40.0 37 37.268344218209755 40.0 38.0 40.0 32.0 40.0 38 37.312908182865925 40.0 38.0 40.0 32.0 40.0 39 37.09719554360353 40.0 38.0 40.0 32.0 40.0 40 36.99308490203611 38.0 38.0 40.0 32.0 40.0 41 37.128505570495584 40.0 38.0 40.0 32.0 40.0 42 36.992508643872455 38.0 38.0 40.0 32.0 40.0 43 37.29177871686515 40.0 38.0 40.0 32.0 40.0 44 37.166538609296964 40.0 38.0 40.0 32.0 40.0 45 37.23184786784479 40.0 38.0 40.0 32.0 40.0 46 36.79504417979255 38.0 38.0 40.0 32.0 40.0 47 36.87725701114099 38.0 38.0 40.0 32.0 40.0 48 36.918939684978874 38.0 38.0 40.0 32.0 40.0 49 36.81809450633884 38.0 38.0 40.0 32.0 40.0 50 36.61352285824049 38.0 38.0 40.0 32.0 40.0 51 36.06377257011141 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 2.0691028589466143 2201 2 1.2263516865079396 2201 3 1.4671897546897625 2201 4 2.1519463609307365 2201 5 1.1253303120490656 2201 6 1.6768471545815444 2201 7 1.1683554292929301 2201 8 1.52317708333333 2201 9 1.6176609848484844 2201 10 1.942401695526705 2201 11 1.91372655122656 2201 12 2.026512333152958 2201 13 1.633536255411265 2201 14 2.0137631899350765 2201 15 1.791752908549796 2201 16 2.0979177940115505 2201 17 2.2084071744228027 2201 18 1.159978129509362 2201 19 1.8865665584415439 2201 20 2.268165471681087 2201 21 1.6632119182900453 2201 22 0.8482514880952294 2201 23 1.5881724612193366 2201 24 1.6085976957070685 2201 25 0.8688531520562748 2201 26 2.038990913600294 2201 27 2.080757350288593 2201 28 2.001216405122655 2201 29 0.35833502435065157 2201 30 2.102123917748912 2201 31 1.9957110164141483 2201 32 2.4631144029581407 2201 33 1.4979673971861445 2201 34 1.9788352272727252 2201 35 1.9610868731962512 2201 36 0.7807088744588739 2201 37 1.5678639069264122 2201 38 -1.5097041847041908 2201 39 1.3504052804834075 2201 40 2.1827076569264108 2201 41 1.5242536976911936 2201 42 2.2515698277417258 2201 43 2.302427737193362 2201 44 2.3616832386363527 2201 45 2.6944055510461737 2201 46 2.698740192099571 2201 47 1.5810724431818244 2201 48 2.660288149350656 2201 49 2.4404367334054697 2201 50 2.5397845643939405 2201 51 2.4406791125541147 1101 1 1.0691028589466143 1101 2 -0.749838789682542 1101 3 -1.509000721500719 1101 4 -4.014720305735931 1101 5 -7.350860164141412 1101 6 -4.704105226370835 1101 7 -4.14116838023088 1101 8 -4.072061011904765 1101 9 -1.6442437770562819 1101 10 -3.9385506854256747 1101 11 -2.9672258297258196 1101 12 0.5979409045815274 1101 13 0.2049648268398343 1101 14 -1.5338558576839745 1101 15 -0.7558661390692549 1101 16 -1.1639869678932158 1101 17 -1.3868309208152922 1101 18 -3.292402822871587 1101 19 -5.542004870129887 1101 20 -1.2794535759379642 1101 21 0.23464048971861473 1101 22 -0.008891369047624664 1101 23 0.15960103264790604 1101 24 -1.1533070661976978 1101 25 0.7259960091991289 1101 26 1.6104194850288636 1101 27 -1.6811474116161733 1101 28 0.23931164321788856 1101 29 0.5964302624458853 1101 30 -4.207399891774898 1101 31 0.5671395878427177 1101 32 -2.1321236922799542 1101 33 0.9265388257575751 1101 34 -3.9497362012987054 1101 35 -8.134151222041847 1101 36 -2.3383387445887465 1101 37 -4.289278950216442 1101 38 -6.700180375180377 1101 39 -2.8876899576118262 1101 40 -1.0791971049783555 1101 41 -3.7852701118326166 1101 42 -3.677001600829705 1101 43 -1.9118579770923532 1101 44 2.2188260957792068 1101 45 -3.5198801632395416 1101 46 0.27016876352814023 1101 47 0.009643871753254984 1101 48 -4.268283279220775 1101 49 0.13091292388165954 1101 50 0.4445464691558456 1101 51 -2.9164637445887394 2202 1 -2.530897141053387 2202 2 -1.316505456349205 2202 3 -3.8756673881673827 2202 4 -0.24805363906926203 2202 5 -1.6175268308080817 2202 6 -1.4374385597041694 2202 7 1.225498286435787 2202 8 1.2946056547619023 2202 9 1.3890895562770567 2202 10 1.4281159812409925 2202 11 0.9994408369408418 2202 12 1.3979409045815245 2202 13 1.4049648268398371 2202 14 1.8994774756493626 2202 15 1.2774671942640836 2202 16 0.26934636544012136 2202 17 0.779835745851372 2202 18 -2.3257361562049255 2202 19 1.2579951298701104 2202 20 -3.2461202426046256 2202 21 0.23464048971861473 2202 22 -0.008891369047624664 2202 23 1.3596010326479089 2202 24 -2.4199737328643636 2202 25 -3.474003990800874 2202 26 -0.789580514971135 2202 27 0.2521859217171638 2202 28 1.3726449765512214 2202 29 -2.870236404220776 2202 30 2.159266774891769 2202 31 -0.6328604121572852 2202 32 -3.165457025613293 2202 33 -0.4734611742424235 2202 34 -2.049736201298707 2202 35 1.3325154446248177 2202 36 1.2949945887445864 2202 37 2.3107210497835595 2202 38 2.2331529581529566 2202 39 2.3789767090548395 2202 40 0.7541362283549802 2202 41 1.5813965548340505 2202 42 1.6229983991702923 2202 43 1.7881420229076497 2202 44 -4.181173904220792 2202 45 -1.619880163239543 2202 46 -1.3298312364718612 2202 47 -3.190356128246748 2202 48 -2.9682832792207776 2202 49 -0.3024204094516776 2202 50 0.7112131358225113 2202 51 -1.0164637445887408 1102 1 -2.09756380772005 1102 2 1.0834945436507937 1102 3 -6.0090007215007155 1102 4 2.1519463609307365 1102 5 -3.1841934974747446 1102 6 -0.03743855970417087 1102 7 1.0254982864357842 1102 8 1.0946056547618994 1102 9 -4.810910443722946 1102 10 -6.938550685425678 1102 11 -3.8005591630591553 1102 12 -0.5687257620851369 1102 13 -0.1283685064935014 1102 14 0.2994774756493612 1102 15 -0.9225328057359192 1102 16 -6.163986967893212 1102 17 -5.053497587481964 1102 18 -7.7924028228715905 1102 19 -5.042004870129887 1102 20 -4.779453575937964 1102 21 -5.098692843614721 1102 22 -3.0088913690476247 1102 23 -5.17373230068543 1102 24 0.8466929338023022 1102 25 -6.940670657467539 1102 26 -1.2229138483044721 1102 27 -4.347814078282838 1102 28 -3.2606883567821114 1102 29 -3.570236404220779 1102 30 -3.8740665584415694 1102 31 -1.766193745490618 1102 32 -6.96545702561329 1102 33 -11.906794507575757 1102 34 -11.116402867965373 1102 35 1.1991821112914849 1102 36 -8.171672077922079 1102 37 -7.95594561688311 1102 38 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33 -1.448461174242425 2204 34 -1.5747362012987054 2204 35 1.5325154446248206 2204 36 1.7449945887445892 2204 37 1.960721049783558 2204 38 2.133152958152955 2204 39 1.028976709054838 2204 40 0.6291362283549802 2204 41 -3.1186034451659523 2204 42 -0.6770016008297048 2204 43 -2.661857977092353 2204 44 0.7188260957792068 2204 45 -2.7698801632395416 2204 46 -0.47983123647185977 2204 47 -1.115356128246745 2204 48 1.7317167207792252 2204 49 2.297579590548324 2204 50 1.36121313582251 2204 51 1.0835362554112606 2205 1 0.5691028589466143 2205 2 -5.666505456349206 2205 3 0.9493326118326166 2205 4 0.026946360930736546 2205 5 1.9824731691919197 2205 6 0.46256144029582913 2205 7 -1.5995017135642158 2205 8 -1.4053943452381006 2205 9 0.3140895562770538 2205 10 -1.3968840187590104 2205 11 -0.1755591630591553 2205 12 1.0979409045815274 2205 13 -2.4200351731601657 2205 14 -1.3255225243506388 2205 15 -1.5475328057359192 2205 16 0.9193463654401199 2205 17 -1.095164254148628 2205 18 1.1242638437950774 2205 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1.1979409045815288 1215 13 0.00496482683983146 1215 14 0.2994774756493612 1215 15 0.6774671942640822 1215 16 1.66934636544012 1215 17 -1.720164254148628 1215 18 1.274263843795076 1215 19 0.8579951298701118 1215 20 1.153879757395373 1215 21 0.6346404897186133 1215 22 -1.7088913690476275 1215 23 0.15960103264790604 1215 24 0.980026267135635 1215 25 1.3259960091991303 1215 26 -1.4895805149711379 1215 27 -1.0478140782828405 1215 28 0.2726449765512271 1215 29 0.42976359577922096 1215 30 0.5592667748917677 1215 31 0.1671395878427191 1215 32 0.7345429743867129 1215 33 0.9265388257575751 1215 34 1.7502637987012974 1215 35 1.5325154446248206 1215 36 1.4949945887445892 1215 37 1.1107210497835567 1215 38 1.4331529581529523 1215 39 1.5789767090548352 1215 40 1.1541362283549788 1215 41 1.3813965548340477 1215 42 2.022998399170298 1215 43 1.5881420229076468 1215 44 2.018826095779204 1215 45 2.1801198367604613 1215 46 0.8701687635281417 1215 47 1.4096438717532536 1215 48 1.8317167207792266 1215 49 1.2975795905483238 1215 50 0.21121313582251133 1215 51 -1.4164637445887394 1212 1 0.06910285894661428 1212 2 0.750161210317458 1212 3 -2.453445165945162 1212 4 -1.0702758612914849 1212 5 0.649139835858584 1212 6 -3.037438559704171 1212 7 -1.1967239357864372 1212 8 -7.3498387896825434 1212 9 0.6335340007214967 1212 10 -0.7718840187590104 1212 11 -1.8005591630591553 1212 12 -1.7353924287518083 1212 13 -3.1283685064935014 1212 14 -3.256078079906196 1212 15 -2.4780883612914764 1212 16 -7.441764745670991 1212 17 -7.664608698593071 1212 18 -1.0146250450938084 1212 19 -2.3197826479076653 1212 20 0.22054642406203584 1212 21 -0.9875817325036067 1212 22 -0.8977802579365104 1212 23 -3.0626211895743154 1212 24 -1.0421959550865836 1212 25 -4.05178176857865 1212 26 -0.056247181637800736 1212 27 -0.014480744949501911 1212 28 -1.4273550234487757 1212 29 -0.9591252931096648 1212 30 -4.040733225108234 1212 31 -1.9884159677128395 1212 32 0.14565408549782433 1212 33 -0.1845722853535392 1212 34 0.9947082431457375 1212 35 0.754737666847042 1212 36 0.7172168109668107 1212 37 -3.0670567279942205 1212 38 -2.366847041847045 1212 39 -2.221023290945162 1212 40 -0.6903082160894627 1212 41 -1.507492334054838 1212 42 -1.5103349341630405 1212 43 -4.96741353264791 1212 44 -6.003396126443015 1212 45 -5.130991274350652 1212 46 -2.6187201253607455 1212 47 -5.990356128246745 1212 48 -2.2127277236652176 1212 49 -3.591309298340562 1212 50 -3.1110090863997115 1212 51 -3.9720193001442965 1213 1 0.9781937680375208 1213 2 -0.643778183621933 1213 3 1.2334235209235231 1213 4 -0.029871820887443334 1213 5 0.3461095328282866 1213 6 -1.0374385597041709 1213 7 -1.7017744408369424 1213 8 -0.35993979978355384 1213 9 0.18908955627705382 1213 10 -3.2264294733044636 1213 11 -2.0732864357864287 1213 12 -0.4929681863275661 1213 13 -0.8859442640692592 1213 14 1.3903865665584547 1213 15 -0.3770782602813725 1213 16 -2.4215627254689736 1213 17 0.2343812003968253 1213 18 -2.216645247114016 1213 19 -2.0874594155844335 1213 20 0.7356979392135514 1213 21 0.7800950351731615 1213 22 -0.008891369047624664 1213 23 0.7050555781024528 1213 24 0.7254808125901846 1213 25 1.9987232819264023 1213 26 1.3376922123015902 1213 27 -0.5296322601010175 1213 28 0.9362813401875911 1213 29 1.2933999594155878 1213 30 0.7774485930735864 1213 31 1.2944123151154443 1213 32 -0.5109115710678367 1213 33 1.290175189393942 1213 34 1.4593547077922011 1213 35 1.623424535533914 1213 36 1.9495400432900425 1213 37 1.8016301406926516 1213 38 -1.5486652236652247 1213 39 -1.6755687454906152 1213 40 0.02686350108225355 1213 41 0.5632147366522275 1213 42 0.4593620355339283 1213 43 0.40632384108946695 1213 44 0.8551897321428399 1213 45 -1.4744256177849948 1213 46 -1.4571039637445864 1213 47 1.5550984172078017 1213 48 -0.6773741883116813 1213 49 1.4793977723665037 1213 50 -0.25242322781385695 1213 51 1.7653544372294405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 0.0 22 2.0 23 2.0 24 10.0 25 20.0 26 33.0 27 56.0 28 74.0 29 82.0 30 104.0 31 126.0 32 181.0 33 162.0 34 199.0 35 249.0 36 310.0 37 497.0 38 821.0 39 2276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.46041506533436 13.08608762490392 9.435049961568025 51.018447348193696 2 11.890126776796004 15.021129466000769 55.99308490203612 17.095658855167116 3 14.137533615059548 15.117172493276987 50.787552823665 19.957741067998462 4 11.083365347675759 43.44986553976181 25.412985017287742 20.053784095274683 5 11.083365347675759 25.412985017287742 53.66884364195159 9.834805993084903 6 21.5904725316942 55.41682673837879 11.947752593161736 11.04494813676527 7 15.981559738762966 23.376872839031886 17.90242028428736 42.73914713791779 8 15.501344602381867 57.87552823665002 13.580484056857472 13.04264310411064 9 14.92508643872455 14.944295044179793 13.67652708413369 56.45409143296196 10 9.930849020361123 51.61352285824049 21.110257395313102 17.345370726085285 11 20.783711102573953 16.711486746062235 45.52439492892816 16.98040722243565 12 12.44717633499808 18.901267767960046 24.683058009988475 43.968497887053395 13 17.960046100653095 13.830195927775643 50.15366884364195 18.05608912792931 14 13.945447560507107 14.79062620053784 19.227814060699195 52.036112178255856 15 16.711486746062235 16.327314636957357 20.437956204379564 46.523242412600844 16 45.456292026897216 19.116234390009605 16.311239193083573 19.116234390009605 17 17.26853630426431 48.92431809450634 17.55666538609297 16.25048021513638 18 46.17748751440646 21.80176719170188 18.28659239339224 13.734152900499424 19 16.365731847867846 20.687668075297733 17.55666538609297 45.38993469074145 20 17.55666538609297 16.865155589704187 17.01882443334614 48.5593545908567 21 14.329619669611986 18.421052631578945 16.845946984248943 50.40338071456012 22 13.503649635036496 48.155973876296585 18.67076450249712 19.669611986169805 23 13.638109873223204 20.072992700729927 19.13177103342297 47.157126392623894 24 44.56396465616596 19.957741067998462 18.92047637341529 16.557817902420286 25 18.30580099884748 46.36957356895889 18.70918171340761 16.615443718786015 26 47.00345754898194 20.591625048021513 19.266231271609684 13.138686131386862 27 17.633499807913946 50.59546676911256 16.44256626968882 15.328467153284672 28 16.92278140606992 19.65040338071456 15.539761813292355 47.88705339992316 29 14.271993853246254 21.321552055320783 46.21590472531694 18.19054936611602 30 42.73914713791779 20.995005762581638 19.631194775259317 16.634652324241262 31 17.287744909719553 45.90856703803304 20.245870149827123 16.557817902420286 32 16.3465232424126 20.610833653476757 49.02036112178256 14.022281982328083 33 14.368036880522475 19.381482904341144 50.941221667306955 15.309258547829426 34 16.19285439877065 43.21936227429888 22.512485593545907 18.075297733384556 35 13.772570111409912 18.72839031886285 22.013061851709566 45.48597771801767 36 14.003073376872837 48.38647714175951 19.78486361890127 17.825585862466383 37 13.503649635036496 48.866692278140604 18.901267767960046 18.72839031886285 38 15.136381098732233 19.765655013446022 48.05993084902036 17.038033038801384 39 14.752208989627352 18.651555897041874 21.30234344986554 45.29389166346523 40 15.751056473300038 13.964656165962353 51.84402612370342 18.440261237034193 41 12.178255858624663 14.54091432961967 23.30003841721091 49.98079139454475 42 43.0080676142912 15.36688436419516 23.97233960814445 17.65270841336919 43 15.136381098732233 14.406454091432963 51.018056089127924 19.439108720706876 44 15.0787552823665 45.10180560891279 24.932769880906648 14.886669227814062 45 11.52516327314637 17.691125624279678 54.24510180560891 16.53860929696504 46 40.991164041490585 18.689973107952362 22.800614675374568 17.51824817518248 47 12.927391471379176 15.90472531694199 24.529389166346522 46.63849404533231 48 13.23472915866308 15.751056473300038 19.17018824433346 51.84402612370342 49 40.41490587783327 17.65270841336919 21.01421436803688 20.91817134076066 50 11.794083749519785 18.49788705339992 49.250864387245485 20.457164809834804 51 12.274298885900883 16.38494045332309 20.995005762581638 50.34575489819439 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 0.0 3 0.0 4 0.0 5 2.0 6 4.0 7 3.0 8 2.0 9 1.0 10 0.0 11 1.5 12 3.0 13 3.5 14 4.0 15 3.5 16 3.0 17 3.5 18 4.0 19 5.5 20 7.0 21 4.0 22 1.0 23 3.5 24 6.0 25 4.5 26 6.5 27 10.0 28 9.5 29 9.0 30 14.0 31 19.0 32 35.0 33 51.0 34 61.0 35 71.0 36 92.5 37 114.0 38 176.5 39 239.0 40 272.0 41 305.0 42 340.0 43 375.0 44 432.5 45 490.0 46 529.0 47 568.0 48 1294.0 49 2020.0 50 1217.0 51 414.0 52 301.5 53 189.0 54 155.0 55 121.0 56 95.0 57 69.0 58 50.5 59 32.0 60 26.5 61 21.0 62 18.0 63 15.0 64 12.0 65 9.0 66 9.5 67 10.0 68 6.5 69 3.0 70 6.0 71 9.0 72 5.0 73 1.0 74 1.5 75 1.5 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0384172109104879 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.01920860545524395 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 5206.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.95044179792547 #Duplication Level Percentage of deduplicated Percentage of total 1 84.52593917710198 36.304264310411064 2 5.456171735241503 4.6868997310795235 3 2.817531305903399 3.630426431041106 4 1.7889087656529516 3.073376872839032 5 1.744186046511628 3.74567806377257 6 0.5366726296958855 1.3830195927775644 7 0.7155635062611807 2.1513638109873225 8 0.31305903398926654 1.0756819054936613 9 0.26833631484794274 1.0372646945831734 >10 1.6994633273703041 11.313868613138686 >50 0.0 0.0 >100 0.08944543828264759 4.57164809834806 >500 0.0 0.0 >1k 0.044722719141323794 27.02650787552824 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC 1407 27.02650787552824 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG 126 2.4202842873607375 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT 112 2.1513638109873225 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 48 0.9220130618517095 RNA PCR Primer, Index 27 (95% over 21bp) CGCTTGTACAGTGATAGATAAAAAGCCCCGATGTTCAGGGCTTTTTGCGAT 28 0.5378409527468306 No Hit GGGCAAATGTCCCCTTATCTCTCAATGATGGATGATGCAGCAGCTTTGACC 28 0.5378409527468306 No Hit GTGCTGGAGGAAGGGGTGCATCTCAAACAAAGCCAAGCAGTTCTCATTATC 27 0.5186323472915867 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 25 0.48021513638109875 No Hit CCGCTCAGCAATAGACAAAGGCTATTTTGACTTTGCCTTTTCTTGTGCAGC 23 0.44179792547061086 No Hit AAGGAAACTACCCGCATAGCTATGTAGACGTGGTAGTGTAACCACCATGAA 22 0.42258932001536686 No Hit GTTTGCTAATGTCATCTTCTGTTCTTCCGATAACATCGGCAATAGATCTTC 20 0.384172109104879 No Hit GACAAGAGGGTTACTACCCAGGAGAGGGAACTTCTATGTTTGAGACACAGC 20 0.384172109104879 No Hit CATGAATGCCTGTGGAGTGCAGGGGATCCACATCTGAGACTCCTAAGCTAA 19 0.36496350364963503 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 17 0.32654629273914715 No Hit CGTGGGCAATTTCTAACCATGTGCGGTTAATCGCTTGATTTTCGTACAAAA 17 0.32654629273914715 No Hit GGAGAGCATTGAAACAAAGGCAGGTATCCTCTGTGATAACTGGCCCTGTCT 16 0.3073376872839032 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 14 0.2689204763734153 TruSeq Adapter, Index 12 (95% over 21bp) GTGAGGCGGTGAGATTCAACTGTTGTCATGGCAATCAGATTAGCAGGTTCG 13 0.24971187091817135 No Hit CTCCAGCACCCTCAGCACACTTGCTAACTAAATCAGTATCTGGTCTCATAG 13 0.24971187091817135 No Hit GTGCAATAGAAACAGCCAATTTATCAGCCACAGGGTCGAGAAAAGCACCAA 13 0.24971187091817135 No Hit GATAAGGCATCATGTGTTTGCCCTGTAAATTCGACATTGGCATACATGCCA 13 0.24971187091817135 No Hit ATCCAATACTCAAGCCACAAATCAATGTTGCAATCAACGGTAGCACCAATC 12 0.23050326546292738 No Hit GTGTGGCGAGCTTTTGTCAGAAAATATGATCAATGGCAGACCGATTTGTCT 12 0.23050326546292738 No Hit CAATGCTACCGATTGCAAAAAAAATTCAAGCGATACTGATGCATGAAAAAG 12 0.23050326546292738 No Hit CCCAACTGTATTCACTCTTGCTCTTTGCTCAATCAACAACCGATAAGTGTG 11 0.21129466000768343 No Hit GTCTGGTATATACTGAGAATCGTTGTCCACAAAGTCACTGGTTGGGCACCT 11 0.21129466000768343 No Hit ATGGAATGGAGTTGAATGGAACGGAATGGAGTGGAAAGGAATGGAATGGAA 11 0.21129466000768343 No Hit GCTGAGCACCGACCATGAAGGTAATAGCTACAACTGCTATAACCATTATCG 11 0.21129466000768343 No Hit TGCATGAATAGGCTTGAACATTGACGATAAAAGCGTAGAAAGGGACAGTAC 11 0.21129466000768343 No Hit GGACATTTTACTCCTAATTGTTGGGGCAGAATTTTCCTATTCACAGAAGCA 11 0.21129466000768343 No Hit GGAAGAGAATATGCGGGAATACACAAAGATGCGTTCGGGAAGCCTTTTCTA 11 0.21129466000768343 No Hit CAAGTGGAATACTGTACTCAATGCCCCGGCTGAGCAGAAGTATGTGGTGTG 10 0.1920860545524395 No Hit AGACAGGATGAGTACGTTCGTAAGCATGCACGTGACCAGTGATGGCGAAGT 10 0.1920860545524395 No Hit GTGGTGATGTGTTGATGAAAAGAGTGCAACAGTGCCTGGATAGGCAGCTCG 10 0.1920860545524395 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 10 0.1920860545524395 No Hit GTGCTCAACATAGCGCAAATGCATTTTACTGGTAAGTAGGCTATGTATTGT 10 0.1920860545524395 No Hit AATTATTCTTGCAGTGGGAATATTGAGCAAGGTCTGGCCTTTTGAAGAGAA 10 0.1920860545524395 No Hit AGCACAGACAAGGTGGTGTCGTTATGCAGCTAAACCTGTCTCTTATACACA 10 0.1920860545524395 No Hit GTTAGCTAATGTTGCTACTTCCTTCCCTGACTATTGATGATCTGAATGAAT 10 0.1920860545524395 No Hit TACTGGAGCCGATTGTAATGAAATGGACCGATAGCCAGCGTATTGCTGAAG 10 0.1920860545524395 No Hit AAGTAAAAGACGGTGAAAACGCGCTGGCAATTCACACGAAATATTATCAAC 10 0.1920860545524395 No Hit TCAACCAACCAAACAAAAGTTTCTCCATTATCCGTTACCGCCCAGGGAAGA 9 0.17287744909719555 No Hit CATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTC 9 0.17287744909719555 No Hit GGTATACGTTGTTCCAATTGTAATGTAGATGCAAGGCCGCTGTCTTTCATC 9 0.17287744909719555 No Hit GTGCAAGCCTCAGGTGATCAGAAACAAGAAATGGAGAAGTCCAGATCCCAC 9 0.17287744909719555 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 9 0.17287744909719555 No Hit GACGTGCAAGTATTGTTTGTGACGGTAGATCCTGAACGCGATACGCAAGAG 9 0.17287744909719555 No Hit GTATAAGAGACAGGAGGTTCAGTCGACCACGTTAGATCTCGACATAAGAGA 8 0.1536688436419516 No Hit CCAGCACTACCTCTTCATTCCTGTCTATCTTAGATAAGGCATTTCTCAGCT 8 0.1536688436419516 No Hit ATGCTGCTAAATACGCCTCCTCTTTTTACGGACCTTTCCGCGAAGCCTTGA 8 0.1536688436419516 No Hit ATAGCGAAGACTGTTCGCTCATTTTAACAACAGGTGGCACCTGTCTCTTAT 8 0.1536688436419516 No Hit AGTCCGCATCGATCAAACCATTTTCTGCTCTTTCTACTGCCTCGTGTAAAC 8 0.1536688436419516 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 8 0.1536688436419516 No Hit GTCTTTTTCCGTTCATTAAACGCACTGGTTTTGGCGTTAATCTGATCCAAC 8 0.1536688436419516 No Hit CAAAGAGACTGAGCTGTCCCACATTTCTGAAGAAAAGTGAAAAGACAAAGC 7 0.13446023818670766 No Hit GATTGTGCGCGATGACACGCACTTTATATGTAGCCAAGCGGATTTCAAACG 7 0.13446023818670766 No Hit ATTGTCTGACTTGATAATGACTTTATCCCCAGACATGGCGGGCTGTCTCTT 7 0.13446023818670766 No Hit CTAAGGTAGCAAAACAAAAAAAGTCCGCCGTCTCCCTGTCTCTTATACACA 7 0.13446023818670766 No Hit CAATACAAAGCTGCTTCTCCGATGGTAATTGCCGAAAAACGAGCCCTGTCT 7 0.13446023818670766 No Hit GCTTTGACCAAGACATGTTCAGGTGCAGCAATGCTGTCTCTTATACACATC 7 0.13446023818670766 No Hit TCTCATGAATGGAAAGGAGAGAAATGGCAGTTTGAAGGCTGTCTCTTATAC 7 0.13446023818670766 No Hit ACAATGTTCTGCTCTCTTCTACTAATCTGTTTATAGACACCAGCTCTGTGT 7 0.13446023818670766 No Hit ATTTAGGACCTTGCTCCTTGAAGTGTGACCTCCAAGGCCAGCAACATATGC 7 0.13446023818670766 No Hit GGCTTCTTCTGCAAATCAAGCAGCAAAACTACTGGCCTTAAGAGAAGCTTT 7 0.13446023818670766 No Hit AGCAAGCTCAGGGTCTGTACTCTGGGCATTGCCATTTGTTATACCAAGCTT 7 0.13446023818670766 No Hit TGCCCAAAGAGGGGATAAATATGTTGGCATACCTAGAATGCATATGCAGCA 7 0.13446023818670766 No Hit GTCAGAGGACTGGTGTTTTTCTCCTTTTCAGTACAAGGCTGTGAGCACCCA 7 0.13446023818670766 No Hit CTTTGAAGATTTCGTTGCAAACGGGATAATCTTCACCTAAAAGCTAAACGG 7 0.13446023818670766 No Hit GGGTCATTATCTCAGGGTCGTGATCTCAGGTTCATGAGATCAAGCCCCACA 7 0.13446023818670766 No Hit TCCCAGAGCCTGATAAAAACGGTTAGCGCTTCGTTAATACAGATGTAGGTG 7 0.13446023818670766 No Hit AGATTGTGCTTTTGCTGGATATCCGCCCAATAAGGATCACCACCTGTCTCT 6 0.11525163273146369 No Hit GGAGGGGGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTAC 6 0.11525163273146369 No Hit ATCTAATGGGGATGCTTATAAAGGGAGGATCTGGAATGGTCTGTCTGGCAG 6 0.11525163273146369 No Hit ATCCAAGACAAAACCGTCTCGTTTGGCCTTTTTGGCATGGTCTTCGTGCCT 6 0.11525163273146369 No Hit GTAAATACCAGTGTTTTCCAGTCGTTGCAGCATGTTGATACCTGTCTCTTA 6 0.11525163273146369 No Hit GATCGATGTTGAACAAAGTATTGTTGTTGTAGAGTCGGATAAAGCGACTGT 6 0.11525163273146369 No Hit CCCAGGTATTAAGCCCAGTATCCAATAGTTATTTTTCCTGCTCCTCTTTCT 6 0.11525163273146369 No Hit GTGCTATCGTGTTGATGATACGTGACAGAATGCATCCTGTCTCTTATACAC 6 0.11525163273146369 No Hit ATGGCAATGCAGTATTCTTGGGTGACCGTGATTGCTCCATGCCTGTCTCTT 6 0.11525163273146369 No Hit GCTGACGCTTCGCTATTAGGAGTTTGAGCACTCCCTTTCGGTCGTTATGCA 6 0.11525163273146369 No Hit GACATGATGAACAAATCCATCGATTCCGTCAGGAGTATGCGCTGTCTCTTA 6 0.11525163273146369 No Hit GTAGTGAACTCATACGCAGCGACTTCGGAATGCGTTCCTGTGAGTTTGGAC 6 0.11525163273146369 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.01920860545524395 2 0.0 0.0 0.0768344218209758 3 0.0 0.0 0.1536688436419516 4 0.0 0.0 0.2305032654629274 5 0.0 0.0 0.5186323472915866 6 0.0 0.0 0.5954667691125625 7 0.0 0.0 0.6915097963887822 8 0.0 0.0 0.7299270072992701 9 0.0 0.0 0.8643872454859777 10 0.0 0.0 0.9988474836726854 11 0.0 0.0 1.2101421436803688 12 0.0 0.0 1.2677679600461007 13 0.0 0.0 1.2869765655013445 14 0.0 0.0 1.3446023818670765 15 0.0 0.0 1.498271225509028 16 0.0 0.0 1.517479830964272 17 0.0 0.0 1.6135228582404917 18 0.0 0.0 1.7479830964271994 19 0.0 0.0 1.7479830964271994 20 0.0 0.0 1.7479830964271994 21 0.0 0.0 1.7864003073376873 22 0.0 0.0 2.0553207837111027 23 0.0 0.0 2.2089896273530543 24 0.0 0.0 2.28582404917403 25 0.0 0.0 2.3434498655397618 26 0.0 0.0 2.362658470995006 27 0.0 0.0 2.573953131002689 28 0.0 0.0 2.785247791010373 29 0.0 0.0 3.2078371110257393 30 0.0 0.0 3.495966192854399 31 0.0 0.0 4.1298501728774495 32 0.0 0.0 4.821359969266231 33 0.0 0.0 5.5320783711102575 34 0.0 0.0 6.9535151747983095 35 0.0 0.0 8.144448713023435 36 0.0 0.0 9.200922013061852 37 0.0 0.0 10.756819054936612 38 0.0 0.0 12.0630042258932 39 0.0 0.0 13.446023818670765 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 160 0.0 43.59375 45 ATGCCGT 170 0.0 42.35294 44 AATCTCG 175 0.0 38.571426 36 GTATGCC 190 0.0 37.894733 42 ATCTCGT 190 0.0 37.894733 37 TATGCCG 190 0.0 37.894733 43 TCTCGTA 190 0.0 37.894733 38 CGTATGC 190 0.0 37.894733 41 CTCGTAT 190 0.0 37.894733 39 CAATCTC 180 0.0 37.5 35 TTACAAT 180 0.0 37.5 32 ATTACAA 180 0.0 37.5 31 TACAATC 180 0.0 37.5 33 GATTACA 180 0.0 37.5 30 ACAATCT 180 0.0 37.5 34 TCGTATG 195 0.0 36.923073 40 ACTGATT 190 0.0 35.526314 27 TGATTAC 190 0.0 35.526314 29 CTGATTA 190 0.0 35.526314 28 GACTGAT 190 0.0 35.526314 26 >>END_MODULE