FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_74.34100000004cdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_74.34100000004cdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5206
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC140727.02650787552824No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCG1262.4202842873607375No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT1122.1513638109873225No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC480.9220130618517095RNA PCR Primer, Index 27 (95% over 21bp)
CGCTTGTACAGTGATAGATAAAAAGCCCCGATGTTCAGGGCTTTTTGCGAT280.5378409527468306No Hit
GGGCAAATGTCCCCTTATCTCTCAATGATGGATGATGCAGCAGCTTTGACC280.5378409527468306No Hit
GTGCTGGAGGAAGGGGTGCATCTCAAACAAAGCCAAGCAGTTCTCATTATC270.5186323472915867No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC250.48021513638109875No Hit
CCGCTCAGCAATAGACAAAGGCTATTTTGACTTTGCCTTTTCTTGTGCAGC230.44179792547061086No Hit
AAGGAAACTACCCGCATAGCTATGTAGACGTGGTAGTGTAACCACCATGAA220.42258932001536686No Hit
GTTTGCTAATGTCATCTTCTGTTCTTCCGATAACATCGGCAATAGATCTTC200.384172109104879No Hit
GACAAGAGGGTTACTACCCAGGAGAGGGAACTTCTATGTTTGAGACACAGC200.384172109104879No Hit
CATGAATGCCTGTGGAGTGCAGGGGATCCACATCTGAGACTCCTAAGCTAA190.36496350364963503No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC170.32654629273914715No Hit
CGTGGGCAATTTCTAACCATGTGCGGTTAATCGCTTGATTTTCGTACAAAA170.32654629273914715No Hit
GGAGAGCATTGAAACAAAGGCAGGTATCCTCTGTGATAACTGGCCCTGTCT160.3073376872839032No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC140.2689204763734153TruSeq Adapter, Index 12 (95% over 21bp)
GTGAGGCGGTGAGATTCAACTGTTGTCATGGCAATCAGATTAGCAGGTTCG130.24971187091817135No Hit
CTCCAGCACCCTCAGCACACTTGCTAACTAAATCAGTATCTGGTCTCATAG130.24971187091817135No Hit
GTGCAATAGAAACAGCCAATTTATCAGCCACAGGGTCGAGAAAAGCACCAA130.24971187091817135No Hit
GATAAGGCATCATGTGTTTGCCCTGTAAATTCGACATTGGCATACATGCCA130.24971187091817135No Hit
ATCCAATACTCAAGCCACAAATCAATGTTGCAATCAACGGTAGCACCAATC120.23050326546292738No Hit
GTGTGGCGAGCTTTTGTCAGAAAATATGATCAATGGCAGACCGATTTGTCT120.23050326546292738No Hit
CAATGCTACCGATTGCAAAAAAAATTCAAGCGATACTGATGCATGAAAAAG120.23050326546292738No Hit
CCCAACTGTATTCACTCTTGCTCTTTGCTCAATCAACAACCGATAAGTGTG110.21129466000768343No Hit
GTCTGGTATATACTGAGAATCGTTGTCCACAAAGTCACTGGTTGGGCACCT110.21129466000768343No Hit
ATGGAATGGAGTTGAATGGAACGGAATGGAGTGGAAAGGAATGGAATGGAA110.21129466000768343No Hit
GCTGAGCACCGACCATGAAGGTAATAGCTACAACTGCTATAACCATTATCG110.21129466000768343No Hit
TGCATGAATAGGCTTGAACATTGACGATAAAAGCGTAGAAAGGGACAGTAC110.21129466000768343No Hit
GGACATTTTACTCCTAATTGTTGGGGCAGAATTTTCCTATTCACAGAAGCA110.21129466000768343No Hit
GGAAGAGAATATGCGGGAATACACAAAGATGCGTTCGGGAAGCCTTTTCTA110.21129466000768343No Hit
CAAGTGGAATACTGTACTCAATGCCCCGGCTGAGCAGAAGTATGTGGTGTG100.1920860545524395No Hit
AGACAGGATGAGTACGTTCGTAAGCATGCACGTGACCAGTGATGGCGAAGT100.1920860545524395No Hit
GTGGTGATGTGTTGATGAAAAGAGTGCAACAGTGCCTGGATAGGCAGCTCG100.1920860545524395No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC100.1920860545524395No Hit
GTGCTCAACATAGCGCAAATGCATTTTACTGGTAAGTAGGCTATGTATTGT100.1920860545524395No Hit
AATTATTCTTGCAGTGGGAATATTGAGCAAGGTCTGGCCTTTTGAAGAGAA100.1920860545524395No Hit
AGCACAGACAAGGTGGTGTCGTTATGCAGCTAAACCTGTCTCTTATACACA100.1920860545524395No Hit
GTTAGCTAATGTTGCTACTTCCTTCCCTGACTATTGATGATCTGAATGAAT100.1920860545524395No Hit
TACTGGAGCCGATTGTAATGAAATGGACCGATAGCCAGCGTATTGCTGAAG100.1920860545524395No Hit
AAGTAAAAGACGGTGAAAACGCGCTGGCAATTCACACGAAATATTATCAAC100.1920860545524395No Hit
TCAACCAACCAAACAAAAGTTTCTCCATTATCCGTTACCGCCCAGGGAAGA90.17287744909719555No Hit
CATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTC90.17287744909719555No Hit
GGTATACGTTGTTCCAATTGTAATGTAGATGCAAGGCCGCTGTCTTTCATC90.17287744909719555No Hit
GTGCAAGCCTCAGGTGATCAGAAACAAGAAATGGAGAAGTCCAGATCCCAC90.17287744909719555No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC90.17287744909719555No Hit
GACGTGCAAGTATTGTTTGTGACGGTAGATCCTGAACGCGATACGCAAGAG90.17287744909719555No Hit
GTATAAGAGACAGGAGGTTCAGTCGACCACGTTAGATCTCGACATAAGAGA80.1536688436419516No Hit
CCAGCACTACCTCTTCATTCCTGTCTATCTTAGATAAGGCATTTCTCAGCT80.1536688436419516No Hit
ATGCTGCTAAATACGCCTCCTCTTTTTACGGACCTTTCCGCGAAGCCTTGA80.1536688436419516No Hit
ATAGCGAAGACTGTTCGCTCATTTTAACAACAGGTGGCACCTGTCTCTTAT80.1536688436419516No Hit
AGTCCGCATCGATCAAACCATTTTCTGCTCTTTCTACTGCCTCGTGTAAAC80.1536688436419516No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC80.1536688436419516No Hit
GTCTTTTTCCGTTCATTAAACGCACTGGTTTTGGCGTTAATCTGATCCAAC80.1536688436419516No Hit
CAAAGAGACTGAGCTGTCCCACATTTCTGAAGAAAAGTGAAAAGACAAAGC70.13446023818670766No Hit
GATTGTGCGCGATGACACGCACTTTATATGTAGCCAAGCGGATTTCAAACG70.13446023818670766No Hit
ATTGTCTGACTTGATAATGACTTTATCCCCAGACATGGCGGGCTGTCTCTT70.13446023818670766No Hit
CTAAGGTAGCAAAACAAAAAAAGTCCGCCGTCTCCCTGTCTCTTATACACA70.13446023818670766No Hit
CAATACAAAGCTGCTTCTCCGATGGTAATTGCCGAAAAACGAGCCCTGTCT70.13446023818670766No Hit
GCTTTGACCAAGACATGTTCAGGTGCAGCAATGCTGTCTCTTATACACATC70.13446023818670766No Hit
TCTCATGAATGGAAAGGAGAGAAATGGCAGTTTGAAGGCTGTCTCTTATAC70.13446023818670766No Hit
ACAATGTTCTGCTCTCTTCTACTAATCTGTTTATAGACACCAGCTCTGTGT70.13446023818670766No Hit
ATTTAGGACCTTGCTCCTTGAAGTGTGACCTCCAAGGCCAGCAACATATGC70.13446023818670766No Hit
GGCTTCTTCTGCAAATCAAGCAGCAAAACTACTGGCCTTAAGAGAAGCTTT70.13446023818670766No Hit
AGCAAGCTCAGGGTCTGTACTCTGGGCATTGCCATTTGTTATACCAAGCTT70.13446023818670766No Hit
TGCCCAAAGAGGGGATAAATATGTTGGCATACCTAGAATGCATATGCAGCA70.13446023818670766No Hit
GTCAGAGGACTGGTGTTTTTCTCCTTTTCAGTACAAGGCTGTGAGCACCCA70.13446023818670766No Hit
CTTTGAAGATTTCGTTGCAAACGGGATAATCTTCACCTAAAAGCTAAACGG70.13446023818670766No Hit
GGGTCATTATCTCAGGGTCGTGATCTCAGGTTCATGAGATCAAGCCCCACA70.13446023818670766No Hit
TCCCAGAGCCTGATAAAAACGGTTAGCGCTTCGTTAATACAGATGTAGGTG70.13446023818670766No Hit
AGATTGTGCTTTTGCTGGATATCCGCCCAATAAGGATCACCACCTGTCTCT60.11525163273146369No Hit
GGAGGGGGAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTAC60.11525163273146369No Hit
ATCTAATGGGGATGCTTATAAAGGGAGGATCTGGAATGGTCTGTCTGGCAG60.11525163273146369No Hit
ATCCAAGACAAAACCGTCTCGTTTGGCCTTTTTGGCATGGTCTTCGTGCCT60.11525163273146369No Hit
GTAAATACCAGTGTTTTCCAGTCGTTGCAGCATGTTGATACCTGTCTCTTA60.11525163273146369No Hit
GATCGATGTTGAACAAAGTATTGTTGTTGTAGAGTCGGATAAAGCGACTGT60.11525163273146369No Hit
CCCAGGTATTAAGCCCAGTATCCAATAGTTATTTTTCCTGCTCCTCTTTCT60.11525163273146369No Hit
GTGCTATCGTGTTGATGATACGTGACAGAATGCATCCTGTCTCTTATACAC60.11525163273146369No Hit
ATGGCAATGCAGTATTCTTGGGTGACCGTGATTGCTCCATGCCTGTCTCTT60.11525163273146369No Hit
GCTGACGCTTCGCTATTAGGAGTTTGAGCACTCCCTTTCGGTCGTTATGCA60.11525163273146369No Hit
GACATGATGAACAAATCCATCGATTCCGTCAGGAGTATGCGCTGTCTCTTA60.11525163273146369No Hit
GTAGTGAACTCATACGCAGCGACTTCGGAATGCGTTCCTGTGAGTTTGGAC60.11525163273146369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1600.043.5937545
ATGCCGT1700.042.3529444
AATCTCG1750.038.57142636
GTATGCC1900.037.89473342
ATCTCGT1900.037.89473337
TATGCCG1900.037.89473343
TCTCGTA1900.037.89473338
CGTATGC1900.037.89473341
CTCGTAT1900.037.89473339
CAATCTC1800.037.535
TTACAAT1800.037.532
ATTACAA1800.037.531
TACAATC1800.037.533
GATTACA1800.037.530
ACAATCT1800.037.534
TCGTATG1950.036.92307340
ACTGATT1900.035.52631427
TGATTAC1900.035.52631429
CTGATTA1900.035.52631428
GACTGAT1900.035.52631426
Produced by FastQC (version 0.11.2)